Subscribe to RSS
DOI: 10.1055/s-0038-1661267
Dynamics of Platelet Tubulin in Response to Changes in Free Sulfhydryl Groups
Publication History
Received 17 September 1984
Accepted 29 November 1984
Publication Date:
18 July 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/198502/lookinside/thumbnails/10-1055-s-0038-1661267-1.jpg)
Summary
Pools of polymerized and total tubulin were measured in human platelets as a function of free sulfhydryl groups both in acid-soluble and acid-precipitable cell fractions. Changes in free thiols were produced either by storage of platelets at room temperature or by addition of the potent oxidizing agent diazene dicarboxylic acid (diamide) and were correlated with shifts in the dynamic equilibrium between assembled and disassembled microtubules and platelet aggregation. Diamide at concentrations of 0.5 to 5 mM depleted acid soluble SH groups and reduced protein thiols while causing a progressive decrease in polymerized tubulin. Similar changes, although not as severe, were initiated by storage of platelets at room temperature. Platelet aggregation especially that induced by collagen showed a positive correlation with the pool of polymerized tubulin. Our results indicate that the state of oxidation of sulfhydryl groups especially in the acid- precipitable fraction plays an important role in determining the position of equilibrium between polymerized and depolymerized tubulin.
-
References
- 1 Olmsted JB, Borisy GG. Microtubules. Annu Rev Biochem 1973; 42: 507-540
- 2 Steiner M, Ikeda Y. Quantitative assessment of polymerized and depolymerized platelet microtubule changes caused by aggregating agents. J Clin Invest 1979; 63: 443-448
- 3 Oliver JM, Albertini DF, Berlin RD. Effects of glutathione-oxidizing agents on microtubule assembly and microtubule-dependent surface properties of human neutrophils. J Cell Biol 1976; 71: 921-932
- 4 Kuriyama R, Sakai H. Role of tubulin-SH groups in polymerization of microtubules. J Biochem (Tokyo) 1974; 76: 651-654
- 5 Mellon MG, Rebhuhn LI. Sulfhydryls and the in vitro polymerization of tubulin. J Cell Biol 1976; 70: 226-238
- 6 Ikeda Y, Steiner M. Sulfhydryls of platelet tubulin: Their role in polymerization and colchicine binding. Biochemistry 1978; 17: 3454-3459
- 7 Ando Y, Steiner M, Baldini M. Effect of chilling on membrane related functions of platelets. Transfusion 1974; 14: 453-461
- 8 Wilson L. Properties of colchicine binding protein from chick embryo brain. Interaction with vinca alkaloids and podophyllotoxin Biochemistry 1970; 9: 4999-5007
- 9 Castle AG, Crawford N. The [3H]-colchicine-binding properties of platelet tubulin. Int J Biochem 1978; 9: 439-446
- 10 Ando Y, Steiner M, Baldini M. Effect of storage on sulfhydryl and disulfide groups of human platelets. Blood 1974; 43: 121-129
- 11 Murphy S, Gardner FH. Platelet storage at 22° C; metabolic, morphologic, and functional studies. J Clin Invest 1971; 50: 370-377
- 12 Becker GA, Tucelli M, Kunicki T, Chalos MK, Aster RH. Studies of platelet concentrates stored at 22° C and 4° C. Transfusion 1973; 13: 61-68
- 13 Valeri CR, Feingold H. Hemostatic effectiveness of liquid-preserved platelets stored at 4° C or 22° C and freeze-preserved platelets stored with 5 percent DMSO at -15° C or stored with 6 percent DMSO at -80° C. In: Platelets: Production, function, transfusion and storage. Baldini MG, Ebbe S. (Eds) Grune & Stratton Inc; New York: 1974. pp 377-391
- 14 White JG. Platelet microtubules and microfilaments: Effects of cytochalasin B on structure and function. In: Platelet Aggregation. Caen J. (Ed) Masson & Co; Paris: 1971. pp 15-52
- 15 White JG, Rao GH R. Effects of a microtubule stabilizing agent on the response of platelets to vincristine. Blood 1982; 60: 474-483
- 16 Menche D, Israel A, Karpatkin S. Platelets and microtubules: Effect of colchicine and D2O on platelet aggregation and release induced by calcium ionophore A23187. J Clin Invest 1980; 66: 284-291
- 17 Robinson CW, Mason RG, Wagner RH. Effect of sulfhydryl inhibitors on platelet agglutininability. Proc Soc Exp Biol Med 1963; 113: 857-860
- 18 Aledort LM, Troup SB, Weed RI. Inhibition of sulfhydryl-dependent platelet functions by penetrating and non-penetrating analogues of parachloromercuribenzene. Blood 1968; 31: 471-479
- 19 Al-Mondhiry H, Spaet TH. Inhibition of platelet adhesion to collagen by sulfhydryl inhibitors. Proc Soc Exp Biol Med 1970; 135: 878-882
- 20 Ponstingl J, Krauhs E, Little M, Kempf T. Complete amino acid sequence of a-tubulin from porcine brain. Proc Natl Acad Sci USA 1981; 78: 2757-2761
- 21 Krauhs E, Little M, Kempf T, Hofer-Warbinek R, Ade W, Ponstingl H. Complete amino acid sequence of β-tubulin from porcine brain. Proc Natl Acad Sci USA 1981; 78: 4156-4160