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Thromb Haemost 1984; 52(03): 226-229
DOI: 10.1055/s-0038-1661183
Original Article
Schattauer GmbH Stuttgart

Indium-111-Labelled Human Platelets: A Method for Use in Severe Thrombocytopenia

A du P Heyns
The M.R.C. Blood Platelet Research Unit, University of the Orange Free State, Bloemfontein, South Africa
,
P N Badenhorst
The M.R.C. Blood Platelet Research Unit, University of the Orange Free State, Bloemfontein, South Africa
,
P Wessels
The M.R.C. Blood Platelet Research Unit, University of the Orange Free State, Bloemfontein, South Africa
,
H Pieters
The M.R.C. Blood Platelet Research Unit, University of the Orange Free State, Bloemfontein, South Africa
,
H F Kotzè
The M.R.C. Blood Platelet Research Unit, University of the Orange Free State, Bloemfontein, South Africa
,
M G Lötter
The M.R.C. Blood Platelet Research Unit, University of the Orange Free State, Bloemfontein, South Africa
› Author Affiliations
Further Information

Publication History

Received 07 February 1984

Accepted 28 July 1984

Publication Date:
19 July 2018 (online)

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Summary

We describe and evaluate a simple method for labelling autologous human platelets with Indium-111-oxine in patients with severe thrombocytopenia. Twenty patients with immune thrombocytopenia and platelet counts ranging from 5 to 119 × 109/1 were investigated. Platelets were isolated from blood by differential centrifugation, residual platelets were repeatedly washed from the red cell layer and buffy coat and labelled with In 111 in saline. A mean of 55% ± 21 of platelets were harvesteo from the blood, labelled with 49% ± 24 efficiency and 15.8 × 108 labelled platelets reinjected to the patients. Contamination of the platelets with red cells and plasma was low. The labelled platelets were viable as assessed by in vitro aggregation, recovery in the circulation and mean survival time. This method permits quantitative platelet imaging with autologous labelled platelets in patients with severe thrombocytopenia.

Key words

Indium-111-labelled platelets - Platelet labelling in thrombocytopenia - Platelet label method
 

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