The Increased Sensitivity of Platelets to Prostacyclin in Marathon Runners

Summary

Platelet-rich plasma was obtained 24 hr after the race ended from athletes who ran in the London marathon. The platelets were only marginally less sensitive to adrenaline than were those of non-runners using conventional aggregation tests. However, the runners’ platelets were much more sensitive to inhibition by prostacyclin, a prostaglandin synthesized by endothelial cells. It appeared that this effect was due to a greater activity in the platelets of the membrane-bound adenylate cyclase enzyme which generates intracellular cyclic AMP. Cyclic AMP production is known to be stimulated by prostacyclin and to cause the inhibition of platelet aggregation. The results indicate another possible protective effect of exercise against cardiovascular disease which is independent of the known changes in lipoprotein concentrations previously observed in athletes.

Keywords

Marathon runners - Platelet aggregation - Prostacyclin - Lipoproteins

• References

• 1 Wood PD, Haskell W, Klein H, Lewis S, Stem MP, Farquhar JW. The distribution of plasma lipoproteins in middle-aged male runners. Metabolism 1976; 25: 1249-1257
• 2 Hassall DG, Forrest LA. et al Influence of plasma lipoproteins on platelet aggregation in a normal male population. Arteriosclerosis 1983; 3: 332-338
• 3 Hassall DG, Owen JS, Bruckdorfer KR. The aggregation of isolated human platelets in the presence of lipoproteins and prostacyclin. Biochem J 1983; 216: 43-49
• 4 Dimitriadou C, Dessypris A, Louizou C, Mandalaki T. Marathon Run II: Effects on platlet aggregation. Thromb Haemostas 1977; 37: 451-455
• 5 Mandalaki T, Dessypris A, Louizou C, Panayotopoulou C, Dimitriadou C. Marathon Run III: Effects on coagulation, fibrinolysis, platelet aggregation and serum cortisol levels, a 3 year study. Thromb Haemostas 1980; 43: 49-52
• 6 Bierre Knudsen J, Brodthagen U, Gormsen J, Jordal R, Norregard-Hansen K, Paulev PE. Platelet function and fibrinolytic activity following distance running. Scand J Haematol 1982; 29: 425-430
• 7 Burghuber O, Sinzinger H, Silberbauer K, Wolf C, Haber P. Decreased prostacyclin sensitivity of human platelets after jogging and squash. Prostaglandins Med 1981; 6: 127-130
• 8 Shattil SJ, Anaya-Galindo R, Colman RW, Cooper RA. Platelet sensitivity induced by cholesterol incorporation. J Clin Invest 1975; 55: 636-643
• 9 Hatch FT, Lees RS. Practical methods for plasma lipoprotein analysis. Adv Lipid Res 1968; 6: 1-68
• 10 Born GV R. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature (Lond) 1962; 194: 143-155
• 11 Harper JF, Brooker G. Femtomole sensitive radioimmunoassay for cyclic AMP and cyclic GMP after 2’0 acetylation by acetic anhydride in aqueous solution. J Cyclic Nucleotide Res 1975; 1: 207-218
• 12 Richmond W. Preparation and properties of cholesterol oxidase from Nocardia sp. and its application to the assay of total cholesterol in serum. Clin Chem 1973; 19: 135-156
• 13 Bartlett GR. Phosphorus assay in column chromatography. J Biol Chem 1959; 234: 466-468
• 14 Christie WW. Lipid analysis. Pergamon Press; Oxford: 1973
• 15 Rodbell M. The role of hormope receptors and GTP-regulatory proteins in membrane transduction. Nature (London) 1980; 284: 17-22
• 16 Hildebrandt JD, Seklura RD, Codman J, Iyengar R, Manclark CR, Bimbaumer L. Stimulation and inhibition of adenylyl cyclase mediated by distinct regulatory proteins. Nature (London) 1983; 302: 706-709
• 17 Wong SK-F, Martin BR. The role of a guanine nucleotide-binding protein in the activation of rat fiver plasma-membrane adenylate cyclase by forskolin. Biochem J 1983; 216: 753-759