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Thromb Haemost 1984; 51(02): 212-216
DOI: 10.1055/s-0038-1661061
Original Article
Schattauer GmbH Stuttgart

Identification of Subpopulations of Human Urinary Plasminogen Activators

David A Hart
The Departments of Microbiology and Pediatrics, University of Texas Health Center at Dallas, Dallas, Texas, U.S.A.
,
Robert Kramer
The Departments of Microbiology and Pediatrics, University of Texas Health Center at Dallas, Dallas, Texas, U.S.A.
,
Witold Cieplak
The Departments of Microbiology and Pediatrics, University of Texas Health Center at Dallas, Dallas, Texas, U.S.A.
› Author Affiliations
Further Information

Publication History

Received 08 August 1983

Accepted 30 January 1984

Publication Date:
19 July 2018 (online)

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Summary

Enzymes functioning as plasminogen activators in commercial urokinase preparations and individual human urine concentrates were subjected to affinity chromatography on columns of lentil lectin-sepharose, ricin-sepharose, wheat germ agglutinin-sephar-ose, lotus lectin-sepharose and concanavalin A-sepharose.

Chromatography of the enzymes from both sources yielded similar results for all lectins except lentil lectin. Urokinase from several commercial sources was approximately 50% adherent to lentil lectin-sepharose while only 5-10% of the urinary plasminogen activators from individuals was adherent to this lectin. SDS-PAGE followed by zymography indicated that the observed differences between commercial and individual samples could be due to the presence in urine concentrates of subpopulations of plasminogen activators which were absent from the commercial samples.

Keywords

Plasminogen activators - Lectin affinity chromatography - Fibrinplate method
 

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