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Thromb Haemost 1982; 48(02): 169-172
DOI: 10.1055/s-0038-1657249
Original Article
Schattauer GmbH Stuttgart

Surface Labeling of Human Platelets by Reductive Methylation

Martin O Spycher
The Theodor Kocher Institute, University of Berne, Berne, Switzerland
,
Kenneth J Clemetson
The Theodor Kocher Institute, University of Berne, Berne, Switzerland
,
Ernst F Lüscher
The Theodor Kocher Institute, University of Berne, Berne, Switzerland
› Institutsangaben
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Publikationsverlauf

Received 06. Mai 1982

Accepted 02. August 1982

Publikationsdatum:
13. Juli 2018 (online)

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Summary

A simple, reproducible method for the specific labeling of the surface proteins of human platelets with tritium is described. The labeled platelets were analyzed by two-dimensional gel electrophoresis (isoelectric focusing/sodium dodecyl sulfate-poly-acrylamide gel electrophoresis) followed by fluorography and the results compared with those obtained by conventional methods. Reductive methylation gave an intense labeling of membrane glycoproteins. There was no cross-linking of the labeled membrane proteins by formaldehyde nor could clear evidence be found that inner proteins were labeled by permeation of the reagents through the plasma membrane. As well as previously described platelet membrane glycoproteins, several others could be detected that were not invariably seen using labeling techniques.

Keywords

Human platelets - Surface-labeling - Reductive methylation - Two-dimensional gel electrophoresis
 

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