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Thromb Haemost 1982; 48(01): 101-103
DOI: 10.1055/s-0038-1657227
Original Article
Schattauer GmbH Stuttgart

Thrombin Inhibition and Thrombin Generation by Cultured Aortic Endothelial and Smooth Muscle Cells from Minipig

B Kirchhof
1   The Department of Internal Medicine, University of Münster, Federal Republic of Germany
,
J Grünwald
2   The institute for Arteriosclerosis Research at the University of Münster, Münster, Federal Republic of Germany
› Author Affiliations
Further Information

Publication History

Received 10 February 1982

Accepted 21 June 1982

Publication Date:
13 July 2018 (online)

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Summary

Endothelial and smooth muscle cells cultured from minipig aorta were examined for their inhibitory activity on thrombin and for their thrombin generating capacity.

Endothelial cells showed both a thrombin inhibition and an activation of prothrombin in the presence of Ca++, which was enhanced in the presence of phospholipids. Smooth muscle cells showed an activation of prothrombin but at a lower rate. Both coagulation and amidolytic micro-assays were suitable for studying the thrombin-vessel wall interaction.

Keywords

Thrombin generation - Thrombin inhibition - Endothelial cells - Smooth muscle cells
 

  • References

  • 1 Buonassisi V. Sulfated mucopolysaccharide synthesis and secretion in endothelial cell cultures. Exp Cell Res 1973; 76: 363-368
    CrossrefPubMedGoogle Scholar
  • 2 Chan V, Chan TK. Antithrombin III in fresh and cultured human endothelial cells. A natural anticoagulant from the vascular endothelium. Thromb Res 1979; 15: 209-213
    CrossrefPubMedGoogle Scholar
  • 3 Glimelius B, Busch C, Höök M. Binding of heparin on the surface of cultured human endothelial cells. Thromb Res 1978; 12: 773-782
    CrossrefPubMedGoogle Scholar
  • 4 Awbrey BJ, Hoak JC, Owen WG. Binding of human thrombin to cultured human endothelial cells. J Biol Chem 1979; 254: 4092-4095
    PubMedGoogle Scholar
  • 5 Björck C, Larsson R, Olsson P, Rothman U. Uptake and inactivation of thrombin by fresh, glutardialdehyde or heparin treated human umbilical cord vein endothelium. Thromb Res 1981; 21: 603-610
    CrossrefPubMedGoogle Scholar
  • 6 Simmer RL, Baker JB, Cunningham DD. Direct linkage of thrombin to its cell surface receptors in different cell types. J Supramol Struc 1979; 12: 245-257
    CrossrefPubMedGoogle Scholar
  • 7 Maynard JR, Dreyer BE, Stemerman MB, Pitlich FA. Tissue factor coagulant activity of cultured human endothelial and smooth muscle cells and fibroblasts. Blood 1977; 50: 387-396
    PubMedGoogle Scholar
  • 8 Østerud B. The role of endothelial cells and subendothelial components in the initiation of blood coagulation. Haemostasis 1979; 8: 324-331
    PubMedGoogle Scholar
  • 9 Mason RG, Mohammed SF, Saba HI, Chuang HYK, Lee EL, Balis JU. Functions of endothelium. Pathobiol Annu 1979; 2: 1-48
    PubMedGoogle Scholar
  • 10 Galdal KS, St Evensen A. Effects of divalent cations and various vasoactive and haemostatically active agents on the integrity of monolayers of cultured human endothelial cell. Thromb Res 1981; 21: 273-284
    CrossrefPubMedGoogle Scholar
  • 11 Mosher DF, Vaheri A. Thrombin stimulates the production and release of major surface associated glycoprotein (fibronectin in cultures of human fibroblasts). Exp Cell Res 1978; 112: 323-334
    CrossrefPubMedGoogle Scholar
  • 12 Jaffe EA, Nachmann RC, Becker CG, Minick CK. Culture of human endothelial cells derived from umbilical veins. J Clin Invest 1973; 52: 2745-2756
    CrossrefPubMedGoogle Scholar
  • 13 Ross R. The smooth muscle cell. J. Cell Biol 1971; 50: 172-186
    CrossrefPubMedGoogle Scholar
  • 14 Kirchhof BRJ, Vermeer C, Hemker HC. The determination of prothrombin using synthetic chromogenic substrates; choice of a suitable activator. Thromb Res 1978; 13: 219-232
    CrossrefPubMedGoogle Scholar
  • 15 Bloom AL, Giddings JC, Sheam SAM. Immunohistological studies of coagulation factors in normal blood vessels and platelets. Thrombos Diathes Haemorrh 1975; 34: 599 (Abstr.)
    PubMedGoogle Scholar