Download PDF
Thromb Haemost 1982; 48(01): 013-017
DOI: 10.1055/s-0038-1657205
Original Article
Schattauer GmbH Stuttgart

The Recovery of Platelet Malondialdehyde Production After a Single Intake of Aspirin - The Effect of Dosage

Hiroshi Takayama
The First Division, Department of Internal Medicine, Faculty of Medicine, Kyoto University, Kyoto, Japan
,
Minoru Okuma
The First Division, Department of Internal Medicine, Faculty of Medicine, Kyoto University, Kyoto, Japan
,
Haruto Uchino
The First Division, Department of Internal Medicine, Faculty of Medicine, Kyoto University, Kyoto, Japan
› Author Affiliations
Further Information

Publication History

Received 08 January 1982

Accepted 04 May 1982

Publication Date:
13 July 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

We studied the recovery pattern of platelet malondialdehyde (MDA) formation after a single intake of various doses of aspirin (ASA). In normal subjects, there were striking differences in the recovery pattern between large doses (1,000 or 500 mg) and small doses (100 or 50 mg) of oral ASA. The 2 day lag phase was present in the large dose group which showed the complete inhibition of the circulating platelets 2 hr after oral ASA but not in the small dose group which showed the incomplete inhibition. The difference of recovery time between these two groups was about 2 days. These data suggest that ASA’s effect on megakaryocytes might affect the recovery pattern of MDA formation by the circulating platelets. In 2 patients with chronic idiopathic thrombocytopenic purpura, the 2 day lag phase was not observed in the recovery even after a large ASA dose.

Keywords

Platelet life span - Aspirin - Malondialdehyde - Thiobarbituric acid reaction - Megakaryocyte
 

  • References

  • 1 Stuart MJ, Murphy S, Oski FA. A simple non radioisotope technic for the determination of platelet life-span. N Engl J Med 1975; 292: 1310-1313
    CrossrefPubMedGoogle Scholar
  • 2 Catalano PM, Smith JB, Murphy S. Platelet recovery from aspirin inhibition in vivo; Differing patterns under various assay conditions. Blood 1981; 57: 99-105
    PubMedGoogle Scholar
  • 3 Mustard JF. Platelet survival. Thromb Haemostas 1978; 40: 154-162
    PubMedGoogle Scholar
  • 4 Kocsis JJ, Hernandovich J, Silver MJ, Smith JB, Ingerman C. Duration of inhibition of platelet prostaglandin formation and aggregation by ingested aspirin or indomethacin. Prostaglandins 1973; 3: 141-144
    CrossrefPubMedGoogle Scholar
  • 5 Burch JW, Stanford N, Majerus PW. Inhibition of platelet prostaglandin synthetase by oral aspirin. J Clin Invest 1978; 61: 314-319
    CrossrefPubMedGoogle Scholar
  • 6 Jafari E, Saleem A, Shaikh BS, Demers LM. Effect of aspirin on prostaglandin synthesis by human platelets. Prostaglandins 1976; 12: 829-835
    CrossrefPubMedGoogle Scholar
  • 7 Szczeklik A, Gryglewski RJ, Grodzinska L, Musial J, Serwonska M, Marcinkiewicz E. Platelet aggregability, thromboxane A2 and malonaldehyde formation following administration of aspirin to man. Thromb Res 1979; 15: 405-413
    CrossrefPubMedGoogle Scholar
  • 8 Ali M, McDonald JWD, Thiessen JJ, Coates PE. Plasma acetylsalicylate and salicylate and platelet cyclooxygenase activity following plain and enteric-coated aspirin. Stroke 1980; 11: 9-13
    CrossrefPubMedGoogle Scholar
  • 9 Demers LM, Budin RE, Shaikh BS. The effects of aspirin on megakaryocyte prostaglandin production. Proc Soc Exp Biol Med 1980; 163: 24-29
    CrossrefPubMedGoogle Scholar
  • 10 Dejena E, Barbieri B, Cerletti C, Livio M, de Gaetano G. Impaired thromboxane production by newly formed platelets after aspirin administration to thrombocytopenic rats. Br J Haematol 1980; 46: 465-469
    CrossrefPubMedGoogle Scholar
  • 11 Worthington RE, Nakeff A. The aspirin effect in rat megakaryocytes and its implication for megakaryocyte differentiation. Blood 1981; 58 (Suppl. 01) 119a
    PubMedGoogle Scholar
  • 12 Yoshimoto T, Yamamoto S, Okuma M, Hayaishi O. Solubilization and resolution of thromboxane synthesizing system from microsomes of bovine blood platelets. J Biol Chem 1977; 252: 5871-5874
    PubMedGoogle Scholar
  • 13 Okuma M, Steiner M, Baldini M. Studies on lipid peroxides in platelets. I. Method of assay and effect of storage. J Lab Clin Med 1970; 75: 283-296
    PubMedGoogle Scholar
  • 14 Takayama H, Okuma M, Uchino H. A simple method for estimation of lipoxygenase and cyclo-oxygenase pathways in human platelets - the use of thiobarbituric acid reaction. Thromb Haemostas 1980; 44: 111-114
    PubMedGoogle Scholar
  • 15 Okuma M, Uchino H. Altered arachidonate metabolism by platelets in patients with myeloproliferative disorders. Blood 1979; 54: 1258-1271
    PubMedGoogle Scholar
  • 16 Silver MJ, Smith JB, Ingerman C, Kocsis JJ. Arachidonic acid-induced platelet aggregation and prostaglandin formation. Prostaglandins 1973; 4: 863-875
    CrossrefPubMedGoogle Scholar
  • 17 Placer ZA, Cushman LL, Johnson BC. Estimation of product of lipid peroxidation (Malonyl Dialdehyde) in biochemical systems. Anal Biochem 1966; 16: 359-364
    CrossrefPubMedGoogle Scholar
  • 18 Peng GW, Gadalla MAF, Smith V, Peng A, Chiou WL. Simple and rapid high-pressure liquid chromatographic simultaneous determination of aspirin, salicylic acid, and salicyluric acid in plasma. J Pharm Sci 1978; 67: 710-712
    CrossrefPubMedGoogle Scholar
  • 19 de Haas HA, Clark SE, Zahavi J, Kakkar VV, White AM. A modified non-radioisotope method for measurement of platelet production time. Br J Haematol 1979; 43: 137-141
    CrossrefPubMedGoogle Scholar
  • 20 Pryor WA, Stanley JP. A suggested mechanism for the production of malondialdehyde during the autoxidation of polyunsaturated fatty acids. Non-enzymatic production of prostaglandin endoperoxides during autoxidation. J Org Chem 1975; 40: 3615
    CrossrefPubMedGoogle Scholar
  • 21 White HL, Glassman AT. Biochemical properties of the prostaglandin/thromboxane synthetase of human blood platelets and comparison with the synthetase of bovine seminal vesicles. Prostaglandins 1976; 12: 811-828
    CrossrefPubMedGoogle Scholar
  • 22 Branehög I, Kutti J, Ridell B, Swalin B, Weinfeld A. The relation of thrombokinetics to bone marrow megakaryocytes in idiopathic thrombocytopenic purpura (ITP). Blood 1975; 45: 551-562
    PubMedGoogle Scholar
  • 23 Karpatkin S. Autoimmune thrombocytopenic purpura. Blood 1980; 56: 329-343
    PubMedGoogle Scholar
  • 24 Hamberg M, Svensson J, Blombäck M, Mettinger KL. Shortened megakaryocyte-platelet regeneration time in patients with ischemic cerebrovascular disease. Thromb Res 1981; 21: 675-679
    CrossrefPubMedGoogle Scholar