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Thromb Haemost 1982; 47(02): 166-172
DOI: 10.1055/s-0038-1657155
Original Article
Schattauer GmbH Stuttgart

A Comparison of Plasminogen Activators Derived from Rat Plasma, Primary Rat Hepatocytes and Isolated Perfused Rat Liver

Yoav Sharoni
The Department of Pharmacology, Wellcome Research Laboratories, Research Triangle Park, North Carolina, U.S.A.
,
Maria C Topal
The Department of Pharmacology, Wellcome Research Laboratories, Research Triangle Park, North Carolina, U.S.A.
,
Patricia R Tuttle
The Department of Pharmacology, Wellcome Research Laboratories, Research Triangle Park, North Carolina, U.S.A.
,
Henry Berger Jr
The Department of Pharmacology, Wellcome Research Laboratories, Research Triangle Park, North Carolina, U.S.A.
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Publikationsverlauf

Received 06. Oktober 1981

Accepted 03. März 1982

Publikationsdatum:
13. Juli 2018 (online)

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Summary

Of the two cell types it was possible to culture from the dissociated rat liver, hepatocytes and Kupffer cells, only the former were fibrinolytically active. Rat hepatocytes during the first 24 hr in culture secreted two plasminogen activators with molecular weights identical to those found in rat plasma, an 80,000-dalton form (PA-80) and a 45,000-dalton form (PA-45). Partially purified preparations of plasminogen activators from both sources were subjected to isoelectric focusing (IEF) to compare characteristics further. There were three distinct peaks of PA-45 in each preparation with isoelectric points of 7.1, 7.2 and 7.4; all electrophoretic forms had the same low affinity to fibrin. PA-80 from both sources displayed similar IEF profiles with forms ranging from pH values of 7 to 8, all with the same high affinity to fibrin. The major form of PA-80 in the plasma preparation had an isoelectric point of 7.9 whereas that in the hepatocyte preparation had an isoelectric point of 7.6. The isolated perfused rat liver was also shown to produce both PA-80 and PA-45 emphasizing the physiological relevance of the findings with hepatocytes. It is concluded that in the rat hepatocytes contribute to the plasma profile with regard to the plasminogen activator content.

Key words

Plasminogen activators - Plasma - Hepatocytes - Isolated liver perfusion - Affinity to fibrin
 

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