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Thromb Haemost 1997; 77(04): 718-724
DOI: 10.1055/s-0038-1656040
Fibrinolysis
Schattauer GmbH Stuttgart

Monoclonal Antibodies against the Human Mannose Receptor that Inhibit the Binding of Tissue-type Plasminogen Activator

Marrie Barrett-Bergshoeff
The Gaubius Laboratory, TNO Prevention and Health, Leiden, The Netherlands
,
Femke Noorman
The Gaubius Laboratory, TNO Prevention and Health, Leiden, The Netherlands
,
Rogier Bos
The Gaubius Laboratory, TNO Prevention and Health, Leiden, The Netherlands
,
Dingeman C Rijken
The Gaubius Laboratory, TNO Prevention and Health, Leiden, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 05 July 1996

Accepted after resubmission 03 December 1996

Publication Date:
11 July 2018 (online)

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Summary

To study the role of the mannose receptor in cellular uptake and degradation of tissue-type plasminogen activator (t-PA), a set of five monoclonal antibodies (Moab) was generated against the mannose receptor isolated from human placental tissue.

All Moab specifically recognised the 175 kDa mannose receptor in a crude placenta extract, as shown in Western blot analysis. By use of im- munohistochemistry, we showed that in human placenta only the Hof- bauer cells (fetal macrophages) express the mannose receptor. Epitope competition experiments indicated that the Moab bound to at least two different epitopes on the receptor molecule. Moab 14-3, 14-5, and 15-2, which are directed against one of these epitopes, strongly inhibited the interaction between the purified mannose receptor and t-PA. These Moab also inhibited mannose receptor-mediated degradation of t-PA by cultured human macrophages. The low density lipoprotein receptor-related protein (LRP) mediated t-PA degradation was not affected by the Moab.

It is concluded that the Moab are useful for studying the expression of the human mannose receptor in Western blot and in immunohisto-chemistry, and for studying the interactions between the human mannose receptor and the mannose-containing ligand t-PA.

 

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