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DOI: 10.1055/s-0038-1653879
Differential Inhibition with Antifibrinolytic Agents of Staphylokinase and Streptokinase Induced Clot Lysis[*]
Publication History
Received 08 December 1994
Accepted after resubmission 31 January 1995
Publication Date:
09 July 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/199505/lookinside/thumbnails/10-1055-s-0038-1653879-1.jpg)
Summary
The inhibitory effects of antifibrinolytic amino acids on clot lysis induced with recombinant staphylokinase (SakSTAR) or with streptokinase (SK) were evaluated in a human plasma milieu in vitro and in a hamster pulmonary embolism model in vivo. Addition of tranexamic acid to a system composed of 60 μ1 125I-fibrin-labeled plasma clots submerged in 0.5 ml human plasma, caused dose-dependent inhibition of lysis; complete lysis in 120 min required 30 nM SakSTAR or 100 nM SK and was reduced to 50% with 0.015 mM or with 0.07 mM tranexamic acid, respectively. Aprotinin also produced dose-dependent inhibition; lysis with SakSTAR or with SK was reduced to 50% of the control value with 8 KIU/ml or with 10 KIU/ml aprotinin, respectively. Thus, in human plasma in vitro the antifibrinolytic potency of tranexamic acid was 5-fold higher towards SakSTAR than towards SK, whereas that of aprotinin was comparable towards both agents.
In hamsters with pulmonary embolism given 0.063 mg/kg SakSTAR or 0.20 mg/kg SK over 30 min, the antifibrinolytic potency of tranexamic acid, administered as a single bolus injection or as a bolus injection followed by continuous infusion, was 8- to 10-fold higher towards SakSTAR than towards SK (50% reduction of clot lysis with SakSTAR at 12.5 mg/kg, as compared to 100-150 mg/kg with SK). In contrast, aprotinin was equipotent towards SakSTAR and SK (50% reduction of clot lysis with 2,000 to 2,700 KlU/kg).
The higher antifibrinolytic potency of tranexamic acid (which prevents binding of plasminogen to fibrin) towards SakSTAR than towards SK is most likely related to the requirement of fibrin-bound plasminogen for efficient fibrinolysis with SakSTAR. Tranexamic acid thus may be a useful and relatively specific antidote to clot lysis with SakSTAR and, at higher concentrations, to clot lysis with SK.
This study was supported by a grant from the “Inter-Universitaire Attrac- tiepolen” of the Belgian Government
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