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Thromb Haemost 1995; 73(04): 706-712
DOI: 10.1055/s-0038-1653845
Original Articles
Vessel Wall
Schattauer GmbH Stuttgart

Augmentation of Vascular Endothelial Barrier Function by Heparin and Low Molecular Weight Heparin

P G Bannon
1   The Applied Research, Heart Research Institute, Sydney, Australia
,
Mi-Jurng Kim
1   The Applied Research, Heart Research Institute, Sydney, Australia
2   Cell Biology Groups, Heart Research Institute, Sydney, Australia
,
R T Dean
2   Cell Biology Groups, Heart Research Institute, Sydney, Australia
,
J Dawes
1   The Applied Research, Heart Research Institute, Sydney, Australia
3   The CRC for Blopharmaceutlcal Research, Darlinghurst, NSW, Australia
› Author Affiliations
Further Information

Publication History

Received 10 May 1994

Accepted after resubmission 13 January 1995

Publication Date:
09 July 2018 (online)

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Summary

Glycosaminoglycans (GAGs) are an important component of endothelial barrier function. Early passage human umbilical vein endothelial cells were grown to confluence on transparent micropore filters and barrier function assessed as transendothelial electrical resistance (TEER) and permeability to albumin and sucrose. Unfractionated heparin and the LMW heparin Clexane decreased endothelial permeability to both sucrose and albumin and increased TEER. Chondroitin 6-sulphate also augmented barrier function, but other GAGs had no effect. Interleukin-1 increased permeability to albumin and sucrose and decreased TEER. Although heparin attenuated the effect of IL-1 on TEER and sucrose permeability, it could not restore the barrier to albumin transfer. Denuded endothelial matrix presented a negligible barrier, which was not enhanced by heparin. When sulphation of endogenous GAGs was inhibited by chlorate, barrier function was compromised and was not restored by exogenous heparin. Thus heparin enhances the barrier function of resting endothelium, but cannot completely overcome the increased permeability resulting from exposure to IL-1 or substitute for endogenous GAGs.

 

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