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Thromb Haemost 1971; 26(02): 264-274
DOI: 10.1055/s-0038-1653674
Originalarbeiten – Original Articles – Travaux Originaux
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The Effect of a Tourniquet on the Kinin-Kininogen System in Blood and Muscle

M Nakahara
1   Department of Orthopaedics, Sapporo Medical College, Sapporo, Japan
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Publication History

Publication Date:
24 July 2018 (online)

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Summary

Dogs had tourniquet applied to their hind limbs for 4 h. Plasma kinin, plasma kininase, plasma kininogen, erythrocyte kininase, sarcoplasm kininase, sarcoplasm kinin forming enzymes, kininase from mitochondrial and lysosomal fractions, and kinin forming enzyme in mitochondrial and lysosomal fractions were examined for relationships between muscle and plasma kinins, before and after application of the tourniquet. The characteristics of kininase and kinin forming enzymes in extracts of mitochondrial and lysosomal fractions were stable to acid-heating. However, sarcoplasm kininase was unstable. A kinin forming enzyme which is stable to acid-heating was found in the plasma obtained after release of the tourniquet. A substance which acts as an accelerator of plasma kininase was present also in extracts from mitochondrial and lysosomal fractions. An increase in plasma kinin after release of the tourniquet was followed by a decrease in plasma kininogen content and by an increase in the activities of plasma kininase and erythrocyte kininase. From the results obtained, it appears that kinin forming enzymes may be released from ischemic muscle into the circulating blood after the release of the tourniquet, along with accelerators of plasma kininase. A mechanism to explain the increase in plasma kinin content is discussed.

 

  • References

  • 1 Abe K, Watanabe N, Kumagai N, Mouri T, Seki T, Yoshinaga K. Estimation of kinin in peripheral blood in man. Tohoku J. exp. Med 89: 103 1966;
    CrossrefPubMedGoogle Scholar
  • 2 Armstrong D, Mills G. L. The reversible cold-indnced activation of the human plasma kinin- forming system between 37° C and 0° C. J. Physiol. (Lond) 179: 89 1965;
    PubMedGoogle Scholar
  • 3 Briseid K, Dryrud O. K, Rinvik F. S. The release of kinins in human plasma substrates. Acta pharmacol (Kbh) 24: 39 1966;
    PubMedGoogle Scholar
  • 4 Briseid K. Personal communication.
    PubMedGoogle Scholar
  • 5 Buluk K, Malofiejew M, Czokalo M. Activation of fibrinolysis and of plasma-kinin system during venostasis. Thrombos. Diathes. haemorrhag. (Stuttg) 14: 500 1965;
    PubMedGoogle Scholar
  • 6 Diniz G. R, Garvalho I. F. A micromethod for determination of bradykininogen under several conditions. Ann. N. Y. Acad. Sci 104: 77 1963;
    PubMedGoogle Scholar
  • 7 De Duve C, Pressman B. C, Gianetto R, Waittiaux R, Appelmans F, Appelmans P. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat liver tissue. Biochem. J 60: 604 1955;
    PubMedGoogle Scholar
  • 8 Edery H, Lewis G. P. Kinin-forming activity and histamine in lymph after tissue injury. J. Physiol. (Lond) 169: 568 1963;
    CrossrefPubMedGoogle Scholar
  • 9 Edery H. Potentiation of the action of bradykinin on smooth muscle by chymotrypsin, chymotrypsinogen and trypsin. Brit. J. Pharmacol. Chemother 22: 371 1964;
    CrossrefPubMedGoogle Scholar
  • 10 Eisen V. Observations on intrinsic kinin-forming factors in human plasma. The effect of acid, acetone, chloroform, heat, and euglobulin separation on kinin formation. J. Physiol. (Lond) 166: 496 1963;
    PubMedGoogle Scholar
  • 11 Greenbaum L. M, Kim K. S. The kinin-forming and kininase activities of rabbit polymorphonuclear leucocytes. Brit. J. Pharmacol 29: 238 1967;
    PubMedGoogle Scholar
  • 12 Hendley E. D, Shiller A. A. Change in capillary permeability during hypoxemic perfusion of rat hindlegs. Amer. J. Physiol 179: 216 1954;
    PubMedGoogle Scholar
  • 13 Jensen K. B, Vennerod A. M. Purification of the main kinin of human urine. Acta Pharmacol. (Kbh) 19: 265 1962;
    PubMedGoogle Scholar
  • 14 KalcJcar H. M. Differential spectrophotometry of purine compounds by means of specific enzymes. III. Studies of the enzymes of purine metabolism. J. biol. Chem 167: 461 1947;
    PubMedGoogle Scholar
  • 15 Kwaan H. C, McFadzean A J. S. On plasma fibrinolytic activity induced by ischemia. Clin. Sci 15: 245 1956;
    PubMedGoogle Scholar
  • 16 Melmon K. L, Cline M. J. Kinins. Amer. J. Med 43: 153 1967;
    CrossrefPubMedGoogle Scholar
  • 17 Melmon K. L, Cline M. J. Interaction of plasma kinins and granulocytes. Nature (Lond) 213: 90 1967;
    CrossrefPubMedGoogle Scholar
  • 18 Melmon K. L, Cline M. J. The interaction of leucocytes and the kinin system. Biochem. Pharmacol Suppl. 271 1968;
    PubMedGoogle Scholar
  • 19 Millican R. C. S35-plasma and erythrocyte distribution in tourniquet-shocked mice. Amer. J. Physiol 179: 513 1954;
    PubMedGoogle Scholar
  • 20 Nahahara M, Sakahashi H. Effect of application of a tourniquet on bleeding factors in dogs. J. Bone J. Surg 49-A: 1345 1967;
    PubMedGoogle Scholar
  • 21 RinviJc S. F, Dyrud O. K, Briseid K. Determination of plasma kininogen, plasma kininase and erythrocyte kininase. Acta Pharmacol. (Kbh) 24: 169 1966;
    PubMedGoogle Scholar
  • 22 Stagni N, de Bernard B. Lysosomal enzyme activity in rat and beef skeletal muscle. Bio- chim. biophys. Acta (Amst) 170: 129 1968;
    PubMedGoogle Scholar
  • 23 Suzuki T, Mizushima Y, Sato T, Iwanaga S. Purification of bovine bradykininogen. J. Biochem 57: 14 1965;
    CrossrefPubMedGoogle Scholar
  • 24 Tokita T. Bradykinin, kinin forming and kininase activities of plasma and tourniquet shock in dogs. Sapporo Med. J 33: 214 1968;
    PubMedGoogle Scholar
  • 25 Vogt W. Kinin formation by plasmin, and indirect process mediated by activation of kallikrein. J. Physiol. (Lond) 170: 153 1964;
    CrossrefPubMedGoogle Scholar