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Thromb Haemost 1981; 46(03): 655-657
DOI: 10.1055/s-0038-1653441
Original Article
Schattauer GmbH Stuttgart

Enhancement of Thrombin-Induced Degradation of Phosphatidylcholine in Reserpinized Rabbit Platelets

Yasuko Watanabe
The Department of Physiological Chemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
,
Masako Soda
The Department of Physiological Chemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
,
Bonro Kobayashi
The Department of Physiological Chemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo, Japan
› Author Affiliations
Further Information

Publication History

Received 19 June 1981

Accepted 01 September 1981

Publication Date:
05 July 2018 (online)

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Summary

When 1-14C-arachidonic acid-labeled, washed platelets from the reserpinized rabbits were exposed to thrombin, the decrease in radioactivity of phospholipids was significantly stimulated as compared with the control platelets. The increase in radioactivity of fatty acids and their oxygenated metabolites was also stimulated. TLC analysis revealed that the stimulation of the decrease in radioactivity of phospholipids was almost exclusively attributed to that of phosphatidylcholine (PC), and that thrombin induced a slight but significant increase in radioactivity of phosphatidyl ethanolamine (PE) in the reserpinized platelets. The results suggest that thrombin-induced degradation of PC is enhanced in the reserpinized platelets, and the overproduced fatty acids would be metabolized to the larger amount of oxygenated products which result in the activation of the platelets. Thrombin-induced mobilization of PC to PE in the reserpinized platelet phospholipids was also suggested.

Keywords

Platelet phospholipids - Reserpine - Thrombin
 

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