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Thromb Haemost 1981; 46(03): 593-596
DOI: 10.1055/s-0038-1653425
Original Article
Schattauer GmbH Stuttgart

Radiolabeling of Fibrinogen Using the Iodogen Technique

Linda C Knight
The Thrombosis Research Center and Department of Biochemistry, Temple University Health Sciences Center, Philadelphia, Pennsylvania, U.S.A
,
Andrei Z Budzynski
The Thrombosis Research Center and Department of Biochemistry, Temple University Health Sciences Center, Philadelphia, Pennsylvania, U.S.A
,
Stephanie A Olexa
The Thrombosis Research Center and Department of Biochemistry, Temple University Health Sciences Center, Philadelphia, Pennsylvania, U.S.A
› Author Affiliations
Further Information

Publication History

Received 16 March 1981

Accepted 24 June 1981

Publication Date:
05 July 2018 (online)

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Summary

The properties of human fibrinogen labeled with 125-Iodine using Iodogen (1, 3, 4, 6-tetrachloro-3α, 6α-diphenylglycoluril) as an oxidizing agent were compared with those of an iodine monochloride labeled counterpart. It was found that thrombin clottability, binding to staphylococci, the relative specific radioactivity of the Aα, Bβ, and γ chains and in vivo clearance from plasma in rabbits were the same in these two labeled fibrinogen preparations. Labeling efficiency was higher when iodogen was used. It is concluded that human fibrinogen labeled with radioiodine using the Iodogen technique is suitable for studies in vitro and in vivo.

Keywords

Fibrinogen - Radioiodination techniques - Iodogen - Radiolabeled proteins
 

  • References

  • 1 Budzynski AZ, Marder VJ, Parker ME, Shames P, Brizuela BS, Olexa SA. Antigenic markers on Fragment DD, a unique plasmic derivative of human crosslinked fibrin. Blood 1979; 54: 794-804
    PubMedSearch in Google Scholar
  • 2 Budzynski AZ, Joseph RT, Olexa SA, Niewiarowski S. Binding functions of the fibrinogen molecule. Fed Proc 1979; 38: 996
    PubMedSearch in Google Scholar
  • 3 Kakkar VV, Nicolaides AN, Renney JT. et al. 125I-labeled fibrinogen test adapted for routine screening for deep-vein thrombosis. Lancet 1970; 1: 540-542
    PubMedSearch in Google Scholar
  • 4 Coleman RE, Krohn KA, Metzger JM. et al. An in vivo evaluation of I-fibrinogen labeled by four different methods. J Lab Clin Med 1974; 83: 977-982
    PubMedSearch in Google Scholar
  • 5 McFarlane AS. In vivo behavior of I131-fibrinogen. J Clin Invest 1963; 42: 346-361
    CrossrefPubMedSearch in Google Scholar
  • 6 Hunter WM, Greenwood FC. Preparation of iodine-131 labeled human growth hormone of high specific activity. Nature (London) 1962; 194: 495-496
    CrossrefPubMedSearch in Google Scholar
  • 7 Rosa U, Scasselati GA, Pennisi F. et al. Labeling of human fibrinogen with I131 by electrolytic iodination. Biochim Biophys Acta 1964; 86: 519-526
    CrossrefPubMedSearch in Google Scholar
  • 8 Marchalonis JJ. An enzymatic method for the trace iodination of immunoglobulins and other proteins. Biochem J 1969; 113: 299-305
    CrossrefPubMedSearch in Google Scholar
  • 9 Fraker PM, Speck Jr JC. Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1, 3, 4, 6-tetrachloro-3α, 6α- diphenylglycoluril. Biochem Biophys Res Commun 1978; 80: 849-857
    CrossrefPubMedSearch in Google Scholar
  • 10 Markwell MA, Fox CF. Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3α, 6α-diphenylglycoluril. Biochemistry 1978; 17: 4807-4817
    CrossrefPubMedSearch in Google Scholar
  • 11 Kazal LA, Amsel S, Miller OP, Tocantins LM. The preparation and some properties of fibrinogen precipitated from human plasma by glycine. Proc Soc Exp Biol Med 1963; 113: 989-994
    CrossrefPubMedSearch in Google Scholar
  • 12 Ratnoff OD, Menzie C. A new method for the determination of fibrinogen in small samples of plasma. J Lab Clin Med 1951; 37: 316-320
    PubMedSearch in Google Scholar
  • 13 Scheidegger JJ. Une microméthode de I’immuno-éléctrophorése. Int Arch Allergy Appl Immunol 1955; 7: 103-110
    CrossrefPubMedSearch in Google Scholar
  • 14 Helmkamp RW, Contreras MA, Izzo MJ. I131-labeling of proteins by the iodine monochloride method. Int J Appl Radiat Isot 1967; 18: 747-764
    CrossrefPubMedSearch in Google Scholar
  • 15 Weber K, Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem 1969; 244: 4406-4412
    PubMedSearch in Google Scholar
  • 16 Niewiarowski S, Budzynski AZ, Lipinski B. Significance of the intact polypeptide chains of human fibrinogen in ADP-induced platelet aggregation. Blood 1977; 49: 635-644
    PubMedSearch in Google Scholar
  • 17 Hawiger J, Hammond DK, Timmons S, Budzynski AZ. Interaction of human fibrinogen with staphylococci: Presence of a binding region on normal and abnormal fibrinogen variants and fibrinogen derivatives. Blood 1978; 51: 799-812
    PubMedSearch in Google Scholar
  • 18 Welch MJ, Krohn KA. Critical review of radiolabeled fibrinogen, its preparation and use. in Radiopharmaceuticals 1975; chapter 51, Society of Nuclear Medicine , New York 493-502
    PubMed
  • 19 Ardaillou N, Larrieu MJ. In vitro studies of radioiodinated fibrinogen. Comparison of the IC1 and enzymatic methods Thromb Res 1974; 5: 327-341
    CrossrefPubMedSearch in Google Scholar
  • 20 Harwig SS L, Harwig JF, Coleman RE. et al. Effect of iodination level on the properties of radioiodinated fibrinogen. Thromb Res 1975; 6: 375-386
    CrossrefPubMedSearch in Google Scholar
  • 21 Krohn K, Sherman L, Welch M. Studies on radioiodinated fibrinogen. I. Physico-chemical properties of the IC1, chloramine-T, and elec-trolytic reaction products. Biochim Biophys Acta 1972; 285: 404-413
    CrossrefPubMedSearch in Google Scholar
  • 22 Atencio AC, Reeve EB. Studies on the metabolism and distribution of fibrinogen in young and older rabbits. II. Results. J Lab Clin Med 1965; 66: 20-33
    PubMedSearch in Google Scholar
  • 23 Nadelhaft I, Lamy F. Fibrinogen turnover in rats as a function of age. J Lab Clin Med 1973; 82: 267-275
    PubMedSearch in Google Scholar
  • 24 Metzger JM, Seeker-Walker RH, Krohn KA. et al. Unsatisfactory biologic behavior of *I-fibrinogen labeled by the chloramine-T method. J Lab Clin Med 1973; 82: 267-275
    PubMedSearch in Google Scholar
  • 25 Bocci V. Efficient labeling of serum proteins with I131 using chloramine-T. Int J Appl Radiat Isot 1964; 15: 449-465
    CrossrefPubMedSearch in Google Scholar
  • 26 Krohn KA, Knight LC, Harwig JF. et al. Differences in the sites of iodination of proteins following four methods of radioiodination. Biochim Biophys Acta 1977; 490: 497-505
    CrossrefPubMedSearch in Google Scholar
  • 27 Ly B, Kierulf P. Radioiodinated human fibrinogen. In vitro effects of increasing iodination Thromb Res 1975; 6: 387-398
    CrossrefPubMedSearch in Google Scholar
  • 28 Regoeczi E. Iodine-labeled fibrinogen: A review. Br J Haematol 1971; 20: 649-663
    CrossrefPubMedSearch in Google Scholar
  • 29 Sherman LA, Harwig S, Hayne OA. Macromolecular complexes formed as the result of chloramine-T radioiodination of proteins. Int J Appl Radiat Isot 1974; 25: 81-85
    CrossrefPubMedSearch in Google Scholar
  • 30 Olexa SA, Budzynski AZ. Evidence for four different polymerization sites involved in human fibrin formation. Proc Natl Acad Sci USA 1980; 77: 1374-1378
    CrossrefPubMedSearch in Google Scholar
  • 31 Phillips HM, York JL. Bovine fibrinogen. I. Effects of amidination on fibrin-monomer aggregation. Biochemistry 1973; 12: 3637-3642
    CrossrefPubMedSearch in Google Scholar
  • 32 Knight LC, Harwig SS L, Welch MJ. In vitro stability and in vivo clearance of fibrinogen or serum albumin labeled with 77Br, 131I or 125I by direct or indirect synthetic methods. J Nucl Med 1977; 18: 282-288
    PubMedSearch in Google Scholar
  • 33 York JL, Blomback B. The sites of the lactoperoxidase-catalyzed iodination of human fibrinogen. J Biol Chem 1979; 254: 8786-8795
    PubMedSearch in Google Scholar
  • 34 Krohn KA, Welch MJ. Studies of radioiodinated fibrinogen. II Lactoperoxidase iodination of fibrinogen and model compounds. Int J Appl Radiat Isot 1974; 25: 315-323
    PubMedSearch in Google Scholar
  • 35 Stevens AM, Noujaim AA, Shysh A, Ediss C. The in vitro behavior of electrolytically radioiodinated fibrinogen. Int J Nucl Med Biol 1978; 5: 83-87
    CrossrefPubMedSearch in Google Scholar
  • 36 Gilbert LR, Wachsman JT. Optimal conditions for the iodination of fibrinogen using immobilized lactoperoxidase. Thromb Res 1975; 6: 387-398
    CrossrefPubMedSearch in Google Scholar