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Thromb Haemost 1981; 46(02): 479-484
DOI: 10.1055/s-0038-1653392
DOI: 10.1055/s-0038-1653392
Original Article
Characterization of a Form of Bovine Factor VIII Which Does Not Aggregate Human Platelets
Weitere Informationen
Publikationsverlauf
Received 10. November 1980
Accepted 04. Mai 1981
Publikationsdatum:
05. Juli 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/198102/lookinside/thumbnails/10-1055-s-0038-1653392-1.jpg)
Summary
Fractionation of partially purified bovine factor VIII on tricalcium citrate columns produces a material which contains high levels of factor VIII procoagulant activity and factor VIII- related antigen, but does not aggregate human platelets. The procoagulant activity can be blocked by human inhibitors of factor VIII: C and by rabbit antibody to bovine factor VIII. Its activity can be increased by thrombin modification. The apparent molecular weight of this form of factor VIII is significantly lower than that of the forms of factor VIII which contain platelet aggregating activity, and it exhibits a higher mobility on agarose electrophoresis.
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