Coagulation Factors and Components of the Fibrinolytic System in Lymph and Blood in Dogs

L Leandoer; S.-E Bergentz; Inga Marie Nilsson

doi:10.1055/s-0038-1651189

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1968; 19(01/02): 129-135
DOI: 10.1055/s-0038-1651189

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

Coagulation Factors and Components of the Fibrinolytic System in Lymph and Blood in Dogs

L Leandoer

¹Department of Surgery I, University of Göteborg, Sahlgrenska Sjukhuset, Göteborg

²Coagulation Laboratory, University of Lund, Allmänna Sjukhuset, Malmö, Sweden

,

S.-E Bergentz

¹Department of Surgery I, University of Göteborg, Sahlgrenska Sjukhuset, Göteborg

²Coagulation Laboratory, University of Lund, Allmänna Sjukhuset, Malmö, Sweden

,

Inga Marie Nilsson

¹Department of Surgery I, University of Göteborg, Sahlgrenska Sjukhuset, Göteborg

²Coagulation Laboratory, University of Lund, Allmänna Sjukhuset, Malmö, Sweden

› Author Affiliations

Abstract

Summary

Thoracic duct lymph of the dog was compared with blood regarding the concentration of various coagulation factors, coagulation properties, and components of the fibrinolytic system.

The recalcification time for lymph was almost twice as long as for blood. The quotients between the concentration of the coagulation factors in the lymph and blood varied considerably. For fibrinogen the lymph/blood concentration ratio was 1/3, for factor V 1/4, for factor VIII 1/4, for factor IX 2/3 and for P & P 1/2.

The fibrinolytic activity in the lymph was lower than in the blood and no split products were found in the lymph. The lymph/blood concentration ratio was 1/2 for plasminogen and 2/3 for the urokinase inhibitor activity.

Full Text

References

References
1 Andersson L, Nilsson I.M, Olow B. Fibrinolytic activity in man during surgery. Thrombos. Diathes. haemorrh. (Stuttg.) 1962; 7: 391
2 Astrup T, Müllertz S. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem 1952; 40: 346
3 Bergström K, Werner B. Proteins in human thoracic duct lymph. Acta chir. scand. 1966; 131: 413
4 Blombäck B, Blombäck M. Purification of human and bovine fibrinogen. Arkiv Kemi 1956; 10: 415
5 Blomstrand R, Nilsson I.M, Dahlbäck O. Coagulation studies on human thoracic duct lymph. Scand. J. clin. Lab. Invest. 1963; 15: 248
6 Brinkhous K.M, Walker S.A. Prothrombin and fibrinogen in lymph. Amer. J. Physiol. 1941; 132: 666
7 Fantl P, Nelson J.F. Coagulation in lymph. J. Physiol. 1953; 122: 33
8 Gram H.C. Studier over fibrinmengden i menniskets blod og plasma. Steen Hasselbacks førlag, Kobenhavn 1921
9 Greig H.B.W. Inhibition of fibrinolysis by alimentary lipaemia. Lancet 1956; II: 16
10 Grotte G. Passage of dextran molecules across the blood-lymph barrier. Acta chir. scand., Suppl. 211 1956
11 Hansen H.G, Aepinus K. Gerinnungsfaktoren in der Lymphe. Thrombos. Diathes. haemorrh. (Stuttg.) 1960; 4: 435
12 Hansen H.G, Kämpfer M. Gerinnungsfaktoren in der Lymphe. Thrombos. Diathes. haemorrh. (Stuttg.) 1962; 7: 106
13 Hedner U, Nilsson I.M. Determination of plasminogen in human plasma by a casein method. Thrombos. Diathes. haemorrh. (Stuttg.) 1965; 14: 545
14 Howell W.H. The coagulation of lymph. Amer. J. Physiol. 1914; 35: 483
15 Jacobsson, B.: To be published.
16 von Kaulla K.N, Pratt E.B. Influence of intravenously administered heparin on clotting of lymph in the dog. Amer. J. Physiol. 1956; 187: 89
17 Langdell R.D, Bowersox L.W, Weaver R.A, Gibson W.S. Coagulation properties of canine thoracic-duct lymph. Amer. J. Physiol. 1960; 199: 626
18 Niléhn J.-E, Robertson B. On the degradation products of fibrinogen or fibrin after infusion of streptokinase in patients with venous thrombosis. Scand. J. Haemat. 1965; 2: 267
19 Nilsson I.M, Blombäck M, von Francken I. On an inherited autosomal haemorrhagic diathesis with antihaemophilic globulin (AHG) deficiency and prolonged bleeding time. Acta med. scand. 1957; 159: 35
20 Nilsson I.M, Blombäck M, Ramgren O. Haemophilia in Sweden. Acta med. scand. 1961; 170: 665
21 Nilsson I.M, Olow B. Fibrinolysis induced by streptokinase in man. Acta chir. scand. 1962; 123: 247
22 Olow B, Nilsson I.M. Determination of fibrinogen and fibrinogenolytic activity. Thrombos. Diathes. haemorrh. (Stuttg.) 1962; 8: 297
23 Owren P.A, Aas K. The control of dicumarol therapy and the quantitative determination of prothrombin and proconvertin. Scand. J. clin. Lab. Invest. 1951; 3: 201
24 Paraskevas M, Nilsson I.M, Martinsson G. A method for determining serum inhibitors of plasminogen activation. Scand. J. clin. Lab. Invest. 1962; 14: 138
25 Stutman L.J, Dumont A.E, Shinowara G.Y. Coagulation factors in human lymph and plasma. Amer. J. med. Sci. 1965; 250: 292
26 Wolf P. A modification for routine laboratory use of Stefaninis method of estimating factor V in human oxalated plasma. J. clin. Path. 1953; 6: 34