Download PDF
Thromb Haemost 1987; 57(03): 259-262
DOI: 10.1055/s-0038-1651112
Original Article
Schattauer GmbH Stuttgart

Testosterone Effects on Biosynthesis of Coagulation Proteins

Michael R Owens
The Department of Medicine, University of Rochester Medical Center and The Department of Medicine, St. Mary’s Hospital, Rochester, New York, USA
,
Catherine D Cimino
The Department of Medicine, University of Rochester Medical Center and The Department of Medicine, St. Mary’s Hospital, Rochester, New York, USA
,
Joan Donnelly
The Department of Medicine, University of Rochester Medical Center and The Department of Medicine, St. Mary’s Hospital, Rochester, New York, USA
› Author Affiliations
Further Information

Publication History

Received 23 June 1986

Accepted after revision 04 February 1987

Publication Date:
06 July 2018 (online)

  PDF Download  Permissions and Reprints

Summary

Studies were performed to assess the effects of testosterone on synthesis of selected coagulation proteins using the isolated rat liver perfused in vitro for 10 hours, as well as effects of testosterone on plasma levels of these same proteins. Pretreatment of castrated male rat liver donors for 14 days with pellets containing placebo or testosterone, 0.5 mg, 5.0 mg or 15.0 mg had no significant effects on cumulative biosynthesis of Factor II, Factor VII, anti thrombin III, plasminogen or fibrinogen. Plasma concentrations of these proteins in liver donor animals were also unchanged by such hormonal manipulations. In contrast, biosynthesis of fibronectin was increased significantly by increasing doses of testosterone, and plasma concentrations of fibronectin in liver donor rats showed a similar effect.

Keywords

Testosterone - Liver perfusion - Coagulation proteins - Fibronectin
 

  • References

  • 1 Laurell CB, Rannevik G. A comparison of plasma protein changes induced by danazol, pregnancy and estrogens. J Clin Endo Metab 1979; 49: 719-725
    CrossrefPubMedGoogle Scholar
  • 2 Nagasawa H, Kim BK, Steiner M, Baldini MG. Inhibition of thrombin-neutralizing activity of antithrombin III by steroid hormones. Thromb Haemostas 1982; 47: 157-161
    PubMedGoogle Scholar
  • 3 Varenherst E, Wallentin L, Risberg B. The effects of orchiectomy, estrogens and cyproterone-acetate on the antithrombin III concentration in carcinoma of the prostate. Urol Res 1981; 9: 25-28
    PubMedGoogle Scholar
  • 4 Small M, MacLean JA, McArdle BM, Bertina RM, Lowe G DO, Forbes CD. Haemostatic effects of stanozolol in elderly medical patients. Thrombos Res 1984; 35: 353-358
    CrossrefPubMedGoogle Scholar
  • 5 Hedlin AM. The effect of oral contraceptive estrogen on blood coagulation and fibrinolysis. Thromb Diath Haemorrh 1975; 33: 370-377
    PubMedGoogle Scholar
  • 6 Matschiner JT, Bell RG. Effect of sex and sex hormones on plasma prothrombin and vitamin K deficiency. Proc Soc Exp Biol Med 1973; 144: 316-320
    CrossrefPubMedGoogle Scholar
  • 7 Jolly DW, Kadis BM, Nelson TE. Estrogen and prothrombin synthesis. The prothrombinogenic effect of estrogen. Biochem Biophys Res Comm 1977; 74: 41-49
    CrossrefPubMedGoogle Scholar
  • 8 Schrogie JJ, Solomon HM, Zieve PD. Effect of oral contraceptives on vitamin K-dependent clotting activity. Clin Pharm Ther 1967; 8: 670-675
    PubMedGoogle Scholar
  • 9 Metta VC, Johnson BC. Effect of feeding vitamin K-dependent diets to female rats. Nutrition 1960; 72: 455-458
    PubMedGoogle Scholar
  • 10 Nishino Y. Hormonal control of prothrombin synthesis in rat liver microsomes with special reference to the role of estradiol, testosterone and prolactin. Arch Toxicol (Suppl. 02) 1979; 397-402
    PubMedGoogle Scholar
  • 11 Jolly DW, Craig C, Nelson TE. Estrogen and prothrombin synthesis: Effect of estrogen on absorption of vitamin K. Am J Physiol 1977; 232: 112-117
    PubMedGoogle Scholar
  • 12 Matschiner JT, Willingham AK. Influence of sex hormones on vitamin K deficiency and epoxidation of vitamin K in the rat. J Nutr 1974; 104: 660-665
    CrossrefPubMedGoogle Scholar
  • 13 Stadel BV. Oral contraceptives and cardiovascular disease. New Eng J Med 1981; 305: 612-618
    CrossrefPubMedGoogle Scholar
  • 14 Wessler S, Gitel SN, Wan LS, Pasternak BS. Estrogen containing oral contraceptive agents. J Am Med Assn 1976; 236: 2179-2182
    CrossrefPubMedGoogle Scholar
  • 15 Zuck TF, Bergin JJ, Raymond JM, Dwyer WR. Implications of depressed antithrombin III activity associated with oral contraceptives. Surg Gyn & Obst 1971; 133: 609-612
    PubMedGoogle Scholar
  • 16 Owens MR, Cimino CD. Estrogen effects hepatic synthesis of plasma coagulation proteins. Blood 1985; 66: 402-406
    PubMedGoogle Scholar
  • 17 Owens MR, Cimino CD. The interaction of salicylate and vitamin K in synthesis of Factor II (prothrombin). Thrombos Res 1983; 31: 135-143
    CrossrefPubMedGoogle Scholar
  • 18 Satyaswaroop PG, Zaino RJ, Moertel R. Estrogen-like effects of tamoxifen on human endometrial carcinoma transplanted into nude mice. Cancer Res 1984; 44: 4006-4010
    PubMedGoogle Scholar
  • 19 Gabriel BW, Sahai G, Anderson WA. Effect of high dose estrogen on growth and steroid receptor activity in DMBA-induced mammary tumors and uteri of rats. J Toxicol & Environ Health 1983; 11: 437-454
    PubMedGoogle Scholar
  • 20 Owens MR, Miller LL, Cimino CD. Synthesis of Factor II antigen by the isolated perfused rat liver. Biochim Biophys Acta 1981; 676: 365
    CrossrefPubMedGoogle Scholar
  • 21 Owens MR, Cimino CD. The inhibitory effects of sodium salicylate on synthesis of Factor VII by the perfused rat liver. Thrombos Res 1980; 18: 839-845
    CrossrefPubMedGoogle Scholar
  • 22 Owens MR, Miller LL. Net biosynthesis of antithrombin III by the isolated rat liver perfused for 12-24 hours. Biochim Biophys Acta 1980; 827: 30-39
    PubMedGoogle Scholar
  • 23 Laurell CB. Electroimmune assay. Scand J Clin Lab Invest 1972; 29 (Suppl. 124) 21-37
    CrossrefPubMedGoogle Scholar
  • 24 Deutsch DG, Mertz ET. Plasminogen: purification from human plasma by affinity chromatography. Science 1970; 170: 1095-1099
    CrossrefPubMedGoogle Scholar
  • 25 Owens MR, Cimino CD. Biosynthesis of plasminogen by the isolated perfused rat liver. J Lab Clin Med 1985; 105: 368-373
    PubMedGoogle Scholar
  • 26 Mancini G, Carbonara AD, Heremans JF. Immunochemical quantitation of antigens by single radial immunodiffusion. Int J Immunochem 1965; 2: 235-254
    CrossrefPubMedGoogle Scholar
  • 27 Fahey JL, McKelvey EM. Quantitative determination of serum immunoglobulin in antibody agar plates. J Immunol 1965; 94: 84-90
    PubMedGoogle Scholar
  • 28 Owens MR, Cimino CD. Synthesis of fibronectin by the isolated perfused rat liver. Blood 1982; 59: 1305-1309
    PubMedGoogle Scholar
  • 29 Snedechor GW, Cochran WG. Statistical Methods 1980. Iowa State University Press; pp 54-58
  • 30 Olson JP, Miller LL, Troup SB. Synthesis of clotting factors by the isolated, perfused rat liver. J Clin Invest 1966; 45: 690-701
    CrossrefPubMedGoogle Scholar
  • 31 Owen CA, Bowie E JW. Generation of coagulation factors V, XI and XII by The isolated rat liver. Haemostasis 1977; 5: 205-212
    PubMedGoogle Scholar
  • 32 Dodds WJ. Storage release and synthesis of coagulation factors in isolated perfused organs. Am J Physiol 1969; 217: 870-883
    PubMedGoogle Scholar
  • 33 Marceau N, Goyette R, Valet JP, Deschenes J. The effect of dexamethasone on formation of fibronectin extracellular matrix by rat hepatocytes in vitro. Exp Cell Res 1980; 125: 497-502
    CrossrefPubMedGoogle Scholar