Responses of Platelets to Strains of Streptococcus sanguis: Findings in Healthy Subjects, Bernard-Soulier, Glanzmann’s, and Collagen-Unresponsive Patients

A H Soberay; M C Herzberg; J D Rudney; H K Nieuwenhuis; J J Sixma; U Seligsohn

doi:10.1055/s-0038-1651098

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1987; 57(02): 222-225
DOI: 10.1055/s-0038-1651098

Original Article

Schattauer GmbH Stuttgart

Responses of Platelets to Strains of Streptococcus sanguis: Findings in Healthy Subjects, Bernard-Soulier, Glanzmann’s, and Collagen-Unresponsive Patients

A H Soberay

¹The School of Dentistry, University of Minnesota, Minneapolis, MN, USA

,

M C Herzberg

¹The School of Dentistry, University of Minnesota, Minneapolis, MN, USA

,

J D Rudney

¹The School of Dentistry, University of Minnesota, Minneapolis, MN, USA

,

H K Nieuwenhuis

²The Department of Hematology, University Hospital Utrecht, The Netherlands

,

J J Sixma

²The Department of Hematology, University Hospital Utrecht, The Netherlands

,

U Seligsohn

³The Institute of Hematology, Ichilov Hospital, Tel Aviv, Israel

› Author Affiliations

Abstract

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Summary

The ability of endocarditis and dental strains of Streptococcus sanguis to induce platelet aggregation in plasma (PRP) from normal subjects were examined and compared to responses of PRP with known platelet membrane glycoprotein (GP) and response defects.

S. sanguis strains differed in their ability to induce normal PRPs to aggregate. Strains that induced PRP aggregation in more than 60% of donors were significantly faster agonists (mean lag times to onset of aggregation less than 6 min) than those strains inducing response in PRPs of fewer than 60% of donors.

Platelets from patients with Bernard-Soulier syndrome aggregated in response to strains of S. sanguis. In contrast, platelets from patients with Glanzmann’s thrombasthenia and from a patient with a specific defect in response to collagen were unresponsive to S. sanguis. These observations show that GPIb and V are not essential, but GPIIb-IIIa and GPIa are important in the platelet response mechanism to S. sanguis. Indeed, the data suggests that the platelet interaction mechanisms of S. sanguis and collagen may be similar.

Keywords

Streptococcus sanguis - Platelet aggregation - Platelet membrane glycoprotein defects

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References

References
1 Houlihan RB. Studies on the adhesion of human blood platelets and bacteria. Blood 1947; Special Issue No 1: 142-154
2 Clawson CC, White JG. Platelet interaction with bacteria. I. Reaction phases and effects of inhibitors. Am J Pathol 1971; 65: 367-380
3 Herzberg MC, Brintzenhofe K, Clawson CC. Aggregation of human platelets and adhesion of Streptococcus sanguis . Infect Immun 1983; 39: 1457-1469
4 Hawiger J, Steckley S, Hammond D, Cheng C, Timmons S, Glick AA, DesPrez RM. Staphylococci-induced human platelet injury mediated by protein A and immunoglobulin G Fc receptor. J Clin Invest 1979; 64: 931-937
5 Zimmerman TS, Spiegelberg HL. Pneumococcus-induced serotonin release from human platelets. J Clin Invest 1975; 56: 828-834
6 Bick RL. Disseminated intravascular coagulation and related syndromes: etiology, pathophysiology, diagnosis, and managment. Am J Hematol 1978; 5: 265-282
7 Clawson CC. The role of platelets in the pathogenesis of endocarditis. Am Heart Assoc Monogr 1979; 52: 24-27
8 Herzberg MC, MacFarlane GD, Delzer PR. Streptococcus sanguis interactions with human platelets. In: Molecular Basis of Oral Microbial Adhesion. Mergenhagen SE, Rosan B. (eds.). p 53-60 Am Soc Microbiol; Washington, D.C.: 1985
9 Sakariassen KS, Nievelstein P RE M, Coller BS, Sixma JJ. The role of platelet membrane glycoproteins Ib and IIb-IIIa in platelet adherence to human artery subendothelium. Br J Haematol 1986; 63: 681-691
10 Bevers EM, Comfurius P, Levy-Toledano S, Enouf J, Nieuwenhuis HH, Caen JP, Qwaal R FA. Platelet prothrombin converting activity in hereditary disorders of platelet function. Br J Haematol 1986; 63: 335-345
11 Seligsohn U, Rososhansky S. A Glanzmann’s thrombasthenia cluster among Iraqi Jews in Israel. Thromb Haemost 1984; 52: 230-231
12 Nieuwenhuis HK, Akkerman J WN, Houdijk W PM, Sixma JJ. Human blood platelets showing no response to collagen fail to express surface glycoprotein Ia. Nature 1985; 318: 470-472
13 Clawson CC, White JG, Herzberg MC. Platelet interaction with bacterial. VI. Contrasting the role of fibrinogen and fibronectin. Am J Hematol 1980; 9: 43-53
14 Jenkins C SP, Meyer D, Dreyfus MD, Larrieu M. von Willebrand Factor and ristocetin. I. Mechanisms of ristocetin-induced platelet aggregation. Br J Haematol 1974; 28: 561-578
15 Winer BJ. Statistical principles in experimental design. 2nd edition. p 261-309 McGraw-Hill; New York: 1971
16 Dixon WJ. BMDP Statistical Software: 1983 Printing with Additions . p 359-387 University of California Press; Berkeley: 1983
17 Sokal RR, Rohlf FJ. Biometry. 2nd edition. p 242-262 W. H. Freeman; San Francisco: 1981
18 Shrout PE, Fleiss JL. Intraclass correlations: Uses in assessing rater reliability. Psychol Bull 1979; 86: 420-428
19 Erickson PR, Herzberg MC. Purification of a platelet interactive antigen from Streptococcus sanguis . Submitted for publication
20 Nieuwenhuis HK, Akkerman J WN, Sakariassen KS, Houdijk W PM, Nievelstein P FE M, Sixma JJ. Human blood platelets with a defect of response to collagen fail to express surface glycoprotein Ia. Thromb Haemost 1985; 54: 124 (Abstr. 0733)
21 Nurden AT, Caen JP. The different glycoprotein abnormalities in Thrombasthenic and Bernard-Soulier platelets. Seminars in Hematol 1979; 16: 234-250
22 Clemetson KJ, McGregor JL, James E, Dechavanne M, Lüscher EF. Characterization of the platelet membrane glycoprotein abnormalities in Bernard-Soulier syndrome and comparison with normal by surface labeling techniques and high resolution two-dimensional gel electrophoresis. J Clin Invest 1982; 70: 304-311
23 Nurden AT, Caen JP. Specific roles for platelet surface glycoproteins in platelet function. Nature 1975; 255: 720-722
24 George JN, Nurden AT, Phillips DR. Molecular defects in interactions of platelets with the vessel wall. N Eng J Med 1984; 311: 1084-1098
25 Erickson PR, Herzberg MC. A collagen-like immunodeterminant on the surface of Streptococcus sanguisinduces platelet aggregation. J Immunol. 1987 (Accepted)
26 Erickson PR, Herzberg MC. Plasma cofactors of Streptococcus sanguis induced platelet-aggregation. Submitted for publication
27 Hawiger J, Kloczewiak M, Timmons S. Platelet-receptor mechanisms for adhesive macromolecules. In Interaction of Platelets with Vessel Wall. Oates JA, Hawiger J, Ross R. (eds.). pp. 1-19 Am Physiol Soc; Bethesda: 1985
28 Coller BS. Activation affects access to the platelet receptor for adhesive glycoproteins. J Cell Biol 1986; 103: 451-456