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Thromb Haemost 1996; 76(06): 0970-0976
DOI: 10.1055/s-0038-1650694
Original Article
Schattauer GmbH Stuttgart

Acetylsalicylic Acid and Sodium Salicylate Inhibit LPS-lnduced NF-kB/c-Rel Nuclear Translocation, and Synthesis of Tissue Factor (TF) and Tumor Necrosis Factor alfa (TNF-a) in Human Monocytes

L T N Osnes
The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway
,
K B Foss
The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway
,
G B Joø
The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway
,
C Okkenhaug
The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway
,
Å-B Westvik
The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway
,
R Øvstebø
The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway
,
P Kierulf
The R & D Group, Dept. of Clinical Chemistry, Ullevaal University Hospital, Oslo, Norway
› Author Affiliations
Further Information

Publication History

Received 29 February 1996

Accepted after resubmission 08 August 1996

Publication Date:
21 August 2018 (online)

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Summary

We have investigated the effects of acetylsalicylic acid and sodium salicylate on the LPS-induced synthesis of the pro-coagulant protein tissue factor (TF) and the pro-inflammatory protein tumor necrosis fac-tor-a (TNF-a), as well as the prostaglandin PGE2 in human monocytes. Both drugs dose-dependently inhibited LPS-induced TF and TNF-a synthesis at the mRNA and the protein level, and reduced PGE2 production. As evidenced by electro mobility shift assay (EMSA) and the use of a NF-kB prototypic probe, these drugs probably exert their inhibitory effects by interference with the nuclear translocation of NF-KB/c-Rel proteins. These data may expand the understanding of the anti-thrombotic and anti-inflammatory effects of these drugs when activation of monocytes occurs.

 

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