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Thromb Haemost 1996; 75(05): 782-790
DOI: 10.1055/s-0038-1650367
Original Article
Schattauer GmbH Stuttgart

Fibrinogen Promotes Adhesion of Monocytic to Human Mesothelioma Cells

Sreerama Shetty
1   The Departments of Medicine, The University of Texas Health Center at Tyler, Tyler, TX, USA
,
Anuradha Kumar
The Departments of Medicine, The University of Texas Health Center at Tyler, Tyler, TX, USA
,
Siegfried Pueblitz
1   The Departments of Medicine, The University of Texas Health Center at Tyler, Tyler, TX, USA
,
Saleh Emri
2   The Departments of Chest Diseases, Hacettepe University School of Medicine, Ankara, Turkey
,
Yucel Gungen
3   Pathology, Hacettepe University School of Medicine, Ankara, Turkey
,
Alice R Johnson
4   Biochemistry and Pathology, The University of Texas Health Center at Tyler, Tyler, TX, USA
,
Steven Idell
1   The Departments of Medicine, The University of Texas Health Center at Tyler, Tyler, TX, USA
› Author Affiliations
Further Information

Publication History

Received 06 September 1995

Accepted after resubmission 29 January 1996

Publication Date:
10 July 2018 (online)

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Summary

Adhesion between monocytic and mesothelioma or pleural meso-thelial cells influences stromal remodeling in pleural neoplasia. We found that cultured monocytic cells (U937) adhere to either human pleural mesothelioma (MS-1) or mesothelial (MeT5A) cells in vitro. 125I-fibrinogen bound specifically and saturably to either cell line, and specific fibrinogen binding increased upon stimulation of these cells with proinflammatory agents such as phorbol myristate (PMA), lipo-polysaccharide (LPS) or tumor necrosis factor (TNF-±). We purified the fibrinogen receptor protein from a membrane fraction of MS-1 cells and identified it by immunoprecipitation as intercellular adhesion molecule (ICAM-1). Anti-ICAM-1 antibody or antisense oligonucleotides inhibited fibrinogen-mediated cell adhesion and binding of 125I-fibrinogen to mesothelioma or mesothelial cells. Cultured monocytic cells adhere to either mesothelioma or mesothelial cells, and the interaction is promoted by fibrinogen binding ICAM-1 at the cell surface. ICAM-1 is expressed by mesothelioma cells and CD 1 lb by macrophages in the fibrinous mesothelioma tumor stroma. The data suggest a common mechanism by which monocytic cells could adhere to either malignant mesothelioma cells or the mesothelial surface in pleural neoplasia.

 

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