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Thromb Haemost 1981; 45(03): 214-218
DOI: 10.1055/s-0038-1650173
Original Article
Schattauer GmbH Stuttgart

Studies in Man and Experimental Animals of a Low Molecular Weight Heparin Fraction

D P Thomas
The National Institute for Biological Standards and Control, Holly Hill, London
,
R E Merton
*   The Glaxo Operations U. K. Limited, Westfield Road, Runcorn, Cheshire, U. K.
,
W E Lewis
The National Institute for Biological Standards and Control, Holly Hill, London
,
T W Barrowcliffe
The National Institute for Biological Standards and Control, Holly Hill, London
› Institutsangaben
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Publikationsverlauf

Received 12. Dezember 1980

Accepted 16. März 1981

Publikationsdatum:
06. Juli 2018 (online)

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Summary

In vitro and in vivo studies were carried out on a commercially prepared low molecular weight heparin fraction. By APTT assay the fraction had a specific activity of half that of unfractionated mucosal heparin, yet retained full potency by anti-Xa assay (both clotting and chromogenic substrate). When administered intravenously to human volunteers, the anti-Xa/APTT ratio remained the same as it was in vitro. However, after subcutaneous injection, the ratio increased and anti-Xa activity could not be fully neutralized ex vivo by PF4. The fraction was as effective as unfractionated heparin in preventing experimental serum-induced thrombosis, suggesting that a heparin fraction with high specific activity by anti-Factor Xa assay compared to APTT activity may be an effective drug for the prophylaxis of venous thrombosis.

Keywords

Heparin - Heparin fraction - Experimental venous thrombosis
 

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