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Thromb Haemost 1993; 70(04): 702-706
DOI: 10.1055/s-0038-1649653
Original Article
Von Willebrand Factor And Endothelial Cells
Schattauer GmbH Stuttgart

Induction of Endothelial Tissue Factor by Endotoxin and Its Precursors

Charles F Moldow
1   The Department of Medicine, Minneapolis VA Medical Center, Minnesota, USA,
,
Ronald R Bach
1   The Department of Medicine, Minneapolis VA Medical Center, Minnesota, USA,
,
Katherine Staskus
2   Department of Microbiology, University of Minnesota, Minneapolis, Minnesota, USA
,
Paul D Rick
3   Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA
› Author Affiliations
Further Information

Publication History

Received 24 January 1993

Accepted after revision 18 May 1993

Publication Date:
05 July 2018 (online)

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Summary

The structural determinants of lipopolysaccharide required for the induction of tissue factor in human umbilical vein endothelial cells were studied. Intact lipid A was essential for the induction of tissue factor whereas the incomplete lipid A precursors lipid IVA and lipid X, as well as monophosphoryl lipid A and acyloxyacyl hydrolase-treated lipopolysaccharide, were unable to induce tissue factor and tissue factor specific mRNA. However, the lipid A precursor, lipid IVA, was able to inhibit LPS-mediated induction of tissue factor; structural determinants distal to lipid A were found to be required for maximal induction of tissue factor activity and tissue factor mRNA. The presence of serum in the assay was found to amplify but was not obligate for tissue factor induction by LPS.

 

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