β₂-Glycoprotein I and Antiphospholipid Antibodies Assays: Are We Running Appropriate Sample-Blanks?

• References

• 1 Gharavi AE, Harris EN, Asherson RA, Hughes GRV. Anticardiolipin antibodies: isotype distribution and phospholipid specificity. Ann Rheum Dis 1987; 48: 1-6
• 2 Loizou S, McCrea JD, Rudge AC, Reynolds R, Boyle CC, Harris EN. Measurement of anti-cardiolipin antibodies by an enzyme-linked immunosorbent assay (ELISA): standardization and quantitation of the results. Clin Exp Immunol 1985; 62: 738-745
• 3 Harris EN, Gharavi AE, Patel SP, Hughes GRV. Evaluation of the anticardiolipin antibody test: report of an international workshop held 4 April 1986. Clin Exp Immunol 1987; 68: 215-222
• 4 Cowchock S, Fort J, Munoz S, Norberg R, Maddrey W. False positive ELISA tests for anticardiolipin antibodies in sera from patients with repeated abortion, rheumatologic disorders and primary biliary cirrhosis: correlation with elevated polyclonal IgM and implications for patients with repeated abortion. Clin Exp Immunol 1988; 73: 289-294
• 5 Galli M, Comfurius P, Maassen C, Hemker HC, de Baets MH, van Breda-Vriesman PJC, Barbui T, Zwaal RFA, Bevers EM. Anticardiolipin antibodies (ACA) directed not to cardiolipin but to a plasma protein cofactor. Lancet 1990; 335: 1544-1547
• 6 Reber G, Tremblet C, Bemard C, Mermillod B, de Moerloose P. Anticardiolipin antibodies and thrombosis: buffer's influence on the detection and quantitation of anticardiolipin antibody measured by ELISA. Thromb Res 1990; 57: 215-226
• 7 Pierangeli SS, Harris EN, Davis SA, DeLorenzo G. β₂-glycoprotein 1 (β₂-GPI) enhances cardiolipin binding activity but is not the antigen for antiphospholipid antibodies. Br J Haematol 1992; 82: 565-570
• 8 Arvieux J, Roussel B, Jacob MC, Colomb MG. Measurement of anti-phospholipid antibodies by ELISA using β₂-glycoprotein I as an antigen. J Immunol Meth 1991; 143: 223-229