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Thromb Haemost 1993; 70(03): 500-505
DOI: 10.1055/s-0038-1649613
Original Article
Platelets
Schattauer GmbH Stuttgart

Cloning of the cDNA Encoding Human Platelet CD36: Comparison to PCR Amplified Fragments of Monocyte, Endothelial and HEL Cells

B Wyler
The INSERM unité 331, Faculté de Médecine Alexis Carrel, Institut Pasteur de Lyon, Lyon, France
,
L Daviet
The INSERM unité 331, Faculté de Médecine Alexis Carrel, Institut Pasteur de Lyon, Lyon, France
,
H Bortkiewicz
The INSERM unité 331, Faculté de Médecine Alexis Carrel, Institut Pasteur de Lyon, Lyon, France
,
J-C Bordet
The INSERM unité 331, Faculté de Médecine Alexis Carrel, Institut Pasteur de Lyon, Lyon, France
,
J L McGregor
1   Stanford Medical School, Division of HematoloSy (S161), Stanford, CA, USA
› Author Affiliations
Further Information

Publication History

Received 16 April 1992

Accepted after revision 14 April 1993

Publication Date:
05 July 2018 (online)

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Summary

Glycoprotein CD36, also known as GPIIIb or GPIV, is a major platelet glycoprotein that bears the newly identified Naka alloantigen. The aim of this study was to clone platelet CD36 and investigate other forms of CD36-cDNA present in monocytes, endothelial and HEL cells. RNA from above mentioned cells were reverse transcribed (RT), using specific primers for CD36, and amplified by the polymerase chain reaction (PCR) technique. Sequencing the different amplified platelet derived cDNA fragments, spanning the whole coding and flanking regions, showed the near identity between platelet and CD36-placenta cDNA. Platelet CD36-cDNA cross-hybridized, in Southern blots, with RT-PCR amplified cDNA originating from monocytes, endothelial and HEL cells. However, monocytes showed a RT-PCR amplified cDNA fragment (561 bp) that was present in platelets and placenta but not on endothelial on HEL-cells. Northern blot analysis of platelet RNA hybridized with placenta CD36 indicated the presence of a major (1.95 kb) and a minor (0.95 kb) transcript. The 1.95 kb transcript was the only one observed on Northern blots of monocytes, endothelial and HEL cells. These results indicate that the structure of CD36 expressed in platelets is similar, with the exception of the 3’ flanking region, to that of placenta. Differences in apparent molecular weight between CD36 and CD36-like glycoproteins may be due to post-translational modifications.

 

  • References

  • 1 Tomiyama Y, Take H, Ikeda H, Mitani T, Furubayashi T, Mizutani H, Yamamoto N, Tandon NN, Sekiguchi S, Jamieson GA, Kurata Y, Yonezawa T, Tarui S. Identification of the platelet-specific alloantigen, Naka, on platelet membrane glycoprotein IV. Blood 1990; 75: 684-687
    PubMedGoogle Scholar
  • 1a Sugimoto Y, Hamada H, Tsukahara S, Noguchi K, Yamaguchi K, Sato M, Tsuruo T. Molecular cloning and characterization of the complementary DNA for the Mr 85,000 protein overexpressed in Adriamycin-resistant human tumor cells. Cancer Res 1993; 53: 2538-2543
    PubMedGoogle Scholar
  • 2 Poldolsak B. Effect of thrombin, chymotrypsin and aggregate gammaglobulins on the human platelet membrane. Thromb Haemostas 1977; 37: 396-406
    PubMedGoogle Scholar
  • 0 Clemetson KJ, McGregor JL. Characterization of platelet glycoproteins. In Platelets in Biology and Pathology III. Maclntyre EE, Gordon IL. (eds) New York: Academic press; 1987: 160-220
  • 4 Asch AS, Barnwell J, Silverstein RI, Nachman RI. Isolation of the thrombospondin membrane receptor. J Clin Invest 1987; 79: 1054-1061
    CrossrefPubMedGoogle Scholar
  • 5 McGregor JL, Catimel B, Parmentier S, Clezardin P, Dechavanne M, Leung LLK. Rapid purification and partial characterization of human platelet glycoprotein IIIb. Interaction with thrombospondin and its role in platelet aggregation. J Biol Chem 1989; 264: 501-506
    PubMedGoogle Scholar
  • 6 Legrand C, Pidard D, Beiso P, Edelman L. Interaction of a monoclonal antibody to glycoprotein IV (CD36) with human platelets and its effect on platelet function. Platelets 1991; 2: 99-105
    CrossrefPubMedGoogle Scholar
  • 7 Tandon NN, Kralisz U, Jamieson GA. Identification and characterization of glycoprotein IV (CD36) as a primary receptor for platelet-collagen adhesion. J Biol Chem 1989; 264: 7576-7583
    PubMedGoogle Scholar
  • 8 Ockenhouse CF, Chulary JD. Plasmodium falciparum sequestration: OKM5 antigen (CD36) mediates cytoadherence of parasitized erythrocytes to a myelomonocytic cell line. J Infect Dis 1988; 157: 584-588
    CrossrefPubMedGoogle Scholar
  • 9 Lian EC-Y, Siddiqui FA, Jamieson GA, Tandon NN. Platelet agglutinating protein p37 cause platelet agglutinating through its binding to membrane glycoprotein IV. Thromb Haemostas 1991; 65: 102-106
    PubMedGoogle Scholar
  • 10 Ockenhouse CF, Magowan C, Chulay JD. Activation of monocytes and platelets by monoclonal antibodies or malaria-infected erythrocytes binding to the CD36 surface receptor in vitro. J Clin Invest 1989; 84: 468-475
    CrossrefPubMedGoogle Scholar
  • 11 Trezzini C, Jungi TW, Spycher MO, Maly FE, Rao P. Evidence from studies using antibody-induced chemiluminescence as a tool to probe signal transduction. Human monocyte CD36 and CD16 are signaling molecules. Immunology 1990; 71: 29-37
    PubMedGoogle Scholar
  • 12 Huang MM, Bolen JB, Barnwell JW, Shattil SJ, Brugge JS. Membrane glycoprotein IV (CD36) is physically associated with the Fyn, Lyn, and Yes protein-tyrosine kinases in human platelets. Proc Natl Acad Sci USA 1991; 88: 7844-7848
    CrossrefPubMedGoogle Scholar
  • 13 Aiken ML, Ginsberg MH, Byers-Ward V, Plow EF. Effects of OKM5, a monoclonal antibody to glycoprotein IV, on platelet aggregation and thrombospondin surface expression. Blood 1990; 76: 2501-2509
    PubMedGoogle Scholar
  • 14 Schüepp BJ, Pfister H, Clemeston KJ, Silverstein RL, Jungi TW. CD36-mediated signal transduction in human monocytes by anti-CD36 antibodies but not by anti-thrombospondin antibodies recognizing cell membrane-bound thrombospondin. Biochem Biophys Res Commun 1990; 175: 263-270
    PubMedGoogle Scholar
  • 15 Imamura N, Inada T, Mtasiwa DM, Kuramoto A. Demonstration of TSP receptor both on the cell-surface and in the cytoplasm of the megakaryoblastic leukemia cell line (MEG-01). Am J Hematol 1989; 32: 78-79
    CrossrefPubMedGoogle Scholar
  • 16 Edelman P, Vinci G, Villeval JL, Vainchenker W, Henri A, Miglierina P, Rouger P, Reviron J, Breton-Gorius J, Sureau C, Edelman L. A monoclonal antibody against an erythrocyte ontogenic antigen identifies fetal and adult erythroid progenitors. Blood 1986; 67: 56-63
    PubMedGoogle Scholar
  • 17 Kieffer N, Bettaieb A, Legrand C, Coulombei J, Vainchenker W, Edelman L, Breton-Gorius J. Developmentally regulated expression of a 78 kDa erythroblast membrane glycoprotein immunologically related to the platelet thrombospondin receptor. Biochem J 1989; 262: 835-842
    CrossrefPubMedGoogle Scholar
  • 18 Greenwalt DE, Watt KWK, So OY, Jiwani N. PAS IV, an integral membrane protein of mammary epithelial cells, is related to platelet and endothelial cell CD36 (GP IV). Biochemistry 1990; 29: 7054-7059
    CrossrefPubMedGoogle Scholar
  • 19 Catimel B, Mc Gregor JL, Hasler T, Greenwalt DE, Howard RJ, Leung LK. Epithelial membrane glcyoprotein PAS-IV is related to platelet glycoprotein IIIb. Binding to thrombospondin but not to malaria infected erythrocytes. Blood 1991; 77: 2649-2655
    PubMedGoogle Scholar
  • 20 Vega MA, Segui-Real B, Garcia JA, Calés C, Rodriguez F, Vanderkerckhove J, Sandoval IV. Cloning, sequencing, and expression of a cDNA encoding rat LIMP II, a novel 74-kDa lysosomal membrane protein related to the surface adhesion protein CD36. J Biol Chem 1991; 266: 16818-16824
    PubMedGoogle Scholar
  • 21 Oquendo P, Hundt E, Lawler J, Seed B. CD36 directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Cell 1989; 58: 95-101
    CrossrefPubMedGoogle Scholar
  • 22 Newman PJ, Gorki J, White II, Gidwitz S, Cretney CJ, Aster RH. Enzymatic amplification of platelet-specific messenger RNA using the polymerase chain reaction. J Clin Invest 1988; 82: 739-743
    CrossrefPubMedGoogle Scholar
  • 23 Wicki AN, Walz A, Gerber-Huber S, Wenger RH, Vornhagen MR, Clemetson KJ. Isolation and characterisation of human blood platelet mRNA and construction of a cDNA library in lambda gt 11. Thromb Haemostas 1989; 61: 448-453
    PubMedGoogle Scholar
  • 24 Djaffar I, Vilette D, Bray PF, Rosa J-P. Quantitative isolation of RNA from human platelets. Thromb Res 1991; 62: 127-136
    CrossrefPubMedGoogle Scholar
  • 25 Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical vein. J Clin Invest 1973; 52: 2745-2756
    CrossrefPubMedGoogle Scholar
  • 26 Kawasaki ES, Wang AM. Detection of gene expression. In PCR Technology. Erich HA. (ed) New York: Stockton Press; 1989: 89-97
  • 27 Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning. A Laboratory Manual. 2.. Cold Spring Harbor: Cold Spring Harbor Laboratory Press; 1989
  • 28 Kraft R, Tardiff J, Krauter KS, Leinwand LA. Using mini-prep plasmid DNA for sequencing double strandet templates with sequenase. Biotechniques 1988; 6: 544-547
    PubMedGoogle Scholar
  • 29 Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 1985; 230: 1350-1354
    CrossrefPubMedGoogle Scholar
  • 30 Sanger F, Nicken S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 1977; 74: 5463-5467
    CrossrefPubMedGoogle Scholar
  • 31 Eisman R, Surrey S, Ramachandran B, Schwarz E, Ponz M. Structural and functional comparison of the genes for human platelet factor 4 and PF4alt . Blood 1990; 76: 336-344
    PubMedGoogle Scholar
  • 32 Bray PF, Leung CS-I, Shuman MA. Human platelets and megakaryocytes contain alternately spliced glycoprotein IIb mRNAs. J Biol Chem 1990; 265: 9587-9590
    PubMedGoogle Scholar
  • 33 Johnston GI, Bliss GA, Newman PJ, McEver RP. Structure of human gene encoding granula membrane protein-140, a member of the selectin family of adhesion receptors for leukocytes. J Biol Chem 1990; 265: 21381-21385
    PubMedGoogle Scholar
  • 34 Hession C, Tizard R, Vassallo C, Schiffer SB, Goff D, Moy P, Chi-Rosso G, Luhowskyj S, Lobb R, Osborn L. Cloning of alternate form of vascular cell adhesion molecule-1 (VCAM1). J Biol Chem 1991; 266: 6682-6685
    PubMedGoogle Scholar
  • 35 Lipski RH, Sobieski DA, Tandon NN, Herman J, Ikeda H, Jamieson GA. Detection of GPIV (CD36) mRNA in Naka-platelets. Thromb Haemostas 1991; 65: 456-457
    PubMedGoogle Scholar
  • 36 Tusynski G, Kowalska MA. Thrombospondin-induced adhesion of human platelets. J Clin Invest 1991; 87: 1387-1394
    CrossrefPubMedGoogle Scholar
  • 37 Kehrel B, Kronenberg A, Schwippert B, Niesing-Bresch D, Niehues U, Tschöpe D, Van de Loo J, Clemetson KJ. Thrombospondin binds normally to glycoprotein IIIb deficient platelets. Biochem Biophys Res Commun 1991; 179: 985-991
    CrossrefPubMedGoogle Scholar
  • 38 Tandon NN, Ockenhouse CF, Greco NJ, Jamieson GA. Adhesive functions of platelets lacking glycoprotein IV (CD36). Blood 1991; 78: 2809-2813
    PubMedGoogle Scholar
  • 39 Knowles II, Tolidjian B, Marboe C, D’Agati V, Grimes M, Chess LJ. Monoclonal anti-human monocyte antibodies OKM1 and OKM5 possess distinctive tissue distributions including differential reactivity with vascular endothelium. Immunol 1984; 132: 2170-2173
    PubMedGoogle Scholar