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Thromb Haemost 1966; 15(03/04): 403-412
DOI: 10.1055/s-0038-1649441
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

A Modified Two-Stage Assay Method for Tissue-Thromboplastin (TPLN)

N. R Kale   *
1   Chemistry Department II, Karolinska Institutet, Stockholm, Sweden
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Publication Date:
27 June 2018 (online)

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Summary

A two-stage method employing purified clotting factors for the assay of tissue-TPLN is described in detail. The rate of prothrombin activation is determined in the presence of optimum amounts of factors V, VII, X and Ca++. The activation of prothrombin is arrested by addition of EDTA or STI, and the thrombin formed is assayed by the clotting method. The method is rapid, sensitive, reproducible and can differentiate between the tissue-TPLN activity of intact lipo-protein and the procoagulant activity of the phospholipids.

* Present Address: Department of Chemistry, University of Poona, Poona-7, India


 

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