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Thromb Haemost 1966; 15(03/04): 327-337
DOI: 10.1055/s-0038-1649434
Originalarbeiten — Original Articles — Travaux Originaux
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Human Antihemophilic Factor (AHF) Purification A Comparison of Two Procedures[*]

J. P Hurt
1   Departments of Pathology and Biochemistry, University of North Carolina, Chapel Hill, North Carolina
,
R. H Wagner   **)
1   Departments of Pathology and Biochemistry, University of North Carolina, Chapel Hill, North Carolina
,
K. M Brinkhous
1   Departments of Pathology and Biochemistry, University of North Carolina, Chapel Hill, North Carolina
› Author Affiliations
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Publication History

Publication Date:
24 July 2018 (online)

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Summary

A method is described for the precipitation from plasma of a human AHF fraction (Fraction AA) with beta alanine. The Fraction AA is further purified by reprecipitation with beta alanine or by adsorption with Florigel or bentonite. Reprecipitation with beta alanine is a convenient step as no additional equipment or reagents are required for the second precipitation. All reagents can be rendered sterile and free of pyrogens. When the precipitates are quantitatively dissolved, the yields are greater than 70% and purification ranges from 300-600 times. The adsorption procedure also has advantages. Adsorption adds only 45 minutes to a 6 hour fractionation. AHF recovery is as high as 70% with a purification of 3,000 to 5,000 times.

*) This investigation was supported by ITSPHS grants HE-01648 and HE-06350 from the National Institutes of Health.


**) This investigator was supported by Public Health Service Research Career Program Award K3-HE-1147.


 

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