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Thromb Haemost 1994; 72(04): 611-616
DOI: 10.1055/s-0038-1648923
Original Article
Schattauer GmbH Stuttgart

The Platelet Adhesion Capacity to Subendothelial Matrix and Collagen in a Flow Model during Storage of Platelet Concentrates for 7 Days

M N Boomgaard
1   The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology of the University of Amsterdam, The Netherlands
,
C W N Gouwerok
1   The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology of the University of Amsterdam, The Netherlands
,
C H E Homburg
1   The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology of the University of Amsterdam, The Netherlands
,
Ph G de Groot
2   The Department of Haematology, University Hospital Utrecht, Utrecht, The Netherlands
,
M J W IJsseldijk
2   The Department of Haematology, University Hospital Utrecht, Utrecht, The Netherlands
,
D de Korte
1   The Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology of the University of Amsterdam, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 15 March 1994

Accepted after resubmission 20 June 1994

Publication Date:
06 July 2018 (online)

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Summary

The influence of storage of platelet concentrates (PC) on the adhesion capacity of platelets was studied. Twenty-four PC, 12 prepared by the buffy coat (BC) method and 12 by the platelet-rich plasma (PRP) method, were stored for 7 days at room temperature. On days 1,3 and 7 of storage, the platelet adhesion capacity to subendothelial matrix (SEM) and collagen was studied in a rectangular perfusion system under flow conditions in conjunction with the platelet aggregation capacity after stimulation and the adenine nucleotide content. The platelet adhesion capacity to collagen was constant until day 3 of storage and decreased to about 80% of the starting value on day 7 of storage. The adhesion capacity to SEM, however, had already decreased on day 3 to about 75% of the value of day 1 and was even more decreased on day 7 to about 45% of the starting value. On day 1, platelets prepared by the BC method displayed a higher adhesion capacity to collagen and a higher aggregation capacity after stimulation by collagen alone or in combination with ADP, compared to platelets prepared by the PRP method. No other significant differences in adhesion or aggregation capacity were observed between the PC prepared by the two different methods. Both platelet adhesion and aggregation response decreased during storage, as did the total adenine nucleotide content. This study shows that platelet function, as measured by the aggregation and adhesion capacity, of platelets prepared by the PRP method is more severely impaired during the first 3 days of storage as compared to the function of platelets prepared by the BC method.

 

  • References

  • 1 Shukla SD, Morrison WJ, Klatchko DM. Response to platelet activating factor in human platelets stored and aged in plasma. Decrease in aggregation, phosphoinositide turnover, and receptor affinity. Transfusion 1989; 29: 528-33
    PubMedGoogle Scholar
  • 2 McGill M, Brindley DC. Effects of storage on platelet reactivity to arterial subendothelium during blood flow. J Lab Clin Med 1979; 94: 370-80
    PubMedGoogle Scholar
  • 3 DiMinno G, Silver MJ, Murphy S. Stored human platelets retain full aggregation potential in response to pairs of aggregating agents. Blood 1982; 59: 563-8
    PubMedGoogle Scholar
  • 4 Moroff G, Chang CH. Aggregation response of human platelets stored at 22° C as platelet-rich plasma. Transfusion 1979; 19: 704-8
    CrossrefPubMedGoogle Scholar
  • 5 Handin RI, Valeri CR. Haemostatic effectiveness of platelets stored at 22° C. N Engl J Med 1971; 285: 538-41
    CrossrefPubMedGoogle Scholar
  • 6 Becker GA, Tuccelli M, Kunicki TJ, Chalos MK, Aster RH. Studies of platelet concentrates stored at 22 and 4° C. Transfusion 1973; 13: 61-8
    CrossrefPubMedGoogle Scholar
  • 7 Slichter SJ, Harker LA. Preparation of platelet concentrates. II. Storage variables influencing platelet viability and function. Br J Haematol 1976; 34: 403-19
    PubMedGoogle Scholar
  • 8 Baumgartner HR, Muggli R. Adhesion and aggregation: morphologic demonstration, quantitation in vivo and in vitro. In: Platelets in biology and pathology Gordon JL. ed North Holland: Amsterdam; 1976. pp 23-60
  • 9 Escolar G, Mazzara R, White JG, Castillo R, Ordinas A. Contribution of perfusion techniques to the evaluation of the hemostatic effectiveness of platelet concentrates. Blood Cells 1992; 18: 403-15
    PubMedGoogle Scholar
  • 10 Packham MA, Mustard JF. Platelet adhesion. In: Progress in hemostasis and thrombosis Speat TH. ed Grune and Stratton; Orlando: 1984. pp 211-88
  • 11 Sakariassen KS, Aarts PAMM, De Groot PG, Houdijk WPM, Sixma JJ. A perfusion chamber developed to investigate platelet interaction in flowing blood with human vessel wall cells, their extracellular matrix, and purified components. J Lab Clin Med 1983; 102: 522-35
    PubMedGoogle Scholar
  • 12 Washitani Y, Irita Y, Yamamoto K, Maeda Y, Yoshinary M. Prevention of acquired defects in platelet function during blood processing. Transfusion 1988; 28: 571-5
    CrossrefPubMedGoogle Scholar
  • 13 Fijnheer R, Modderman PW, Veldman H, Ouwehand WH, Nieuwenhuis HK, Roos D, de Korte D. Detection of platelet activation with monoclonal antibodies and flowcytometry: changes during platelet storage. Transfusion 1990; 30: 20-5
    CrossrefPubMedGoogle Scholar
  • 14 Pietersz RNI, de Korte D, Reesink HW, Dekker WJA, van den Ende A, Loos JA. Storage of whole blood for up to 24 h at ambient temperature prior to component preparation. Vox Sang 1989; 56: 145-50
    CrossrefPubMedGoogle Scholar
  • 15 Pietersz RNI, Loos JA, Reesink HW. Platelet concentrates stored in plasma for 72 hours at 22° C prepared from buffycoats of citrate-phosphate-dex-trose blood collected in a quadruple-bag saline-adenine-glucose-mannitol system. Vox Sang 1985; 49: 81-5
    PubMedGoogle Scholar
  • 16 Slichter SJ, Harker LA. Preparation and storage of platelet concentrates. I. Factors influencing the harvest of viable platelets from whole blood. Br J Haematol 1976; 34: 395-402
    PubMedGoogle Scholar
  • 17 Loos JA. Automation in blood component preparation In: Transfusion Medicine. Recent Technological Advances Murawski K, Peetoom F. ed Alan R. Liss, Inc; New York: 1985. pp 333-11
  • 18 Boomgaard MN, Gouwerok CWN, de Korte D, Loos JA. Platelets stored for 6 days in a polyolefin container in a synthetic medium with minimal plasma carry over. Vox Sang 1994; 66: 18-24
    CrossrefPubMedGoogle Scholar
  • 19 Fratantoni JC, Poindexter BJ, Bonner RF. Quantitative assessment of platelet morphology by light scattering: a potential method for the evaluation of platelets for transfusion. J Lab Clin Med 1984; 102: 620-31
    PubMedGoogle Scholar
  • 20 Kunicki TJ, Tuccelli M, Becker GA, Aster RH. A study of the variables affecting the quality of platelets stored at room temperature. Transfusion 1975; 15: 414-21
    PubMedGoogle Scholar
  • 21 Muggli R, Baumgartner HR, Tschopp TB, Keller H. Automated microden-simetry and protein assays as a measure for platelet adhesion in collagen-coated slides under controlled flow conditions. J Lab Clin Med 1980; 95: 195-207
    PubMedGoogle Scholar
  • 22 Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973; 52: 2745-56
    CrossrefPubMedGoogle Scholar
  • 23 Fijnheer R, Veldman HA, van den Eertwegh AJM, Gouwerok BWN, Homburg CHE, Boomgaard MN, de Korte D, Roos D. In vitro evaluation of buffy-coat-derived platelet concentrates stored in synthetic medium. Vox Sang 1991; 60: 105-12
    PubMedGoogle Scholar
  • 24 de Korte D, Haverkort WA, Roos D, van Gennip AH. Anion-exchange high performance liquid chromatography method for the quantitation of nucleotides in human blood cells. Clin Chim Acta 1985; 148: 185-96
    CrossrefPubMedGoogle Scholar
  • 25 van Gennip AH. Screening for inborn errors of purine and pyrimidine metabolism by bidimensional TLC and HPLC. In: Handbook of chromatography, nucleic acids and related compounds Krustulovic AM. ed CRC Press; Boca Raton: 1987. pp 221-46
  • 26 Sakariassen KS, Bolhuis PA, Blomback M, Thorell L, Blomback B, Sixma JJ. Association between bleeding time and platelet adhesion to the artery subendothelium. Thromb Haemost 1984; 52: 144-7
    Article in Thieme ConnectPubMedGoogle Scholar
  • 27 Balduini CL, Berolino G, Noris P, Piovella F, Sinigaglia F, Bellotti V, Sa-maden A, Torti M, Mazzini G. Defect of platelet aggregation and adhesion induced by autoantibodies against platelet glycoprotein IIIa. Thromb Haemost 1992; 68: 208-13
    Article in Thieme ConnectPubMedGoogle Scholar
  • 28 Weiss HJ, Baumgartner HR, Tschopp TB, Turitto VT, Cohen D. Correction by factor VIII of the impaired platelet adhesion to subendothelium in von Willebrand disease. Blood 1978; 51: 267-79
    PubMedGoogle Scholar
  • 29 de Korte D, Gouwerok CWN, Fijnheer R, Pietersz RNI, Roos D. Depletion of dense granule nucleotides during storage of human platelets. Thromb Haemost 1990; 63: 275-8
    Article in Thieme ConnectPubMedGoogle Scholar
  • 30 Weiss HJ, Tschopp TB, Baumgartner HR. Impaired interaction (adhesion-aggregation) of platelets with subendothelium in the storage pool disease and after aspirin ingestion. A comparison with von Willebrand’s disease. N Engl J Med 1975; 293: 619-23
    PubMedGoogle Scholar
  • 31 Escolar G, Hagert-Whiting K, Bravo ML, White JG. Interaction of longterm stored platelets with vascular subendothelium. J Lab Clin Med 1987; 109: 147-54
    PubMedGoogle Scholar
  • 32 Hirsh J, Glynn MF, Mustard JF. The effect of platelet age on platelet adherence to collagen. J Clin Invest 1968; 47: 466-73
    CrossrefPubMedGoogle Scholar
  • 33 Dhar A, Ganguly P. Altered expression of platelet surface glycoproteins during storage. Br J Haematol 1988; 70: 71-5
    CrossrefPubMedGoogle Scholar
  • 34 Di Minno G, Capitanio AM, Thiagarajan P, Martinez J, Murphy S. Exposure of fibrinogen receptors on fresh and stored platelets by ADP and epinephrine as single agents and as a pair. Blood 1983; 61: 1054-9
    PubMedGoogle Scholar
  • 35 Sakariassen KS, Nievelstein PFEM, Coller BS, Sixma JJ. The role of platelet membrane glycoproteins Ib and IIb-IIIa in platelet adherence to human artery subendothelium. Br J Haematol 1986; 63: 681-91
    CrossrefPubMedGoogle Scholar
  • 36 Kristensen J, Eriksson L, Olsson K, Killander A, Hogman CF. Functional capacity of transfused platelets estimated by the Thrombostat 4000/2. Eur J Haematol 1993; 51: 152-5
    CrossrefPubMedGoogle Scholar
  • 37 Boomgaard MN, Stulp JG, de Korte D, Pietersz RNI, Reesink HW, Huij-gens PC, Loos JA. Effect of pre-incubation at 37° C of platelet concentrates on the post-transfusion platelet adhesion capacity to collagen and fibrinogen. Haemostasis 1994; 23: 327-34
    PubMedGoogle Scholar
  • 38 Gottschall J, Johnston V, Rzad L, Anderson A, Aster R. Importance of white blood cells in platelet storage. Vox Sang 1984; 47: 101-7
    CrossrefPubMedGoogle Scholar
  • 39 Pietersz RNI, de Korte D, Reesink HW, van den Ende A, Dekker WJA, Roos D. Preparation of leukocyte-poor platelet concentrates from buffy coats. III. Effect of leukocyte contamination on storage conditions. Vox Sang 1988; 55: 14-20
    PubMedGoogle Scholar
  • 40 Shanwell A, Larsson S, Aschan J, Ringden O. A randomized trial comparing the use of fresh and stored platelets in the treatment of bone marrow transplant recipients. Eur J Haematol 1992; 49: 77-81
    PubMedGoogle Scholar
  • 41 Leach MF, AuBuchon JP. Effect of storage time on clinical efficacy of single-donor platelet units. Transfusion 1993; 33: 661-4
    CrossrefPubMedGoogle Scholar