Thromb Haemost 1994; 72(01): 151-154
DOI: 10.1055/s-0038-1648827
Original Article
Schattauer GmbH Stuttgart

Circulating Endothelial Cell/Leukocyte Adhesion Molecules in Atherosclerosis

Andrew D Blann
The Department of Surgery, University Hospital of South Manchester, Didsbury, Manchester, UK
,
Charles N McCollum
The Department of Surgery, University Hospital of South Manchester, Didsbury, Manchester, UK
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received 09. November 1993

Accepted after resubmission 02. März 1994

Publikationsdatum:
12. Juli 2018 (online)

Summary

Soluble adhesion molecules E-selectin, intercellular adhesion molecule (sICAM) and vascular cell adhesion molecule (sVCAM) were measured alongside von Willebrand factor (vWf) in 40 patients with peripheral vascular disease (PVD), 43 with ischaemic heart disease (IHD) and in equal numbers of age and sex matched asymptomatic controls. Increased vWf was found in patients with IHD (p = 0.0008) and in patients with PVD (p = 0.0001) relative to their respective controls but levels did not differ between the two patient groups. Raised sICAM was found in both PVD (p = 0.0003) and IHD (p = 0.0059) compared to their respective controls and was higher in PVD than in IHD (p = 0.0088). In the subjects taken as a whole, there was no correlation between vWf and sICAM. Levels of soluble E-sel-ectin and sVCAM did not differ in patients or controls. These data suggest that soluble ICAM may be useful as an index of endothelial cell activation in clinical manifestations of atherosclerosis.

 
  • References

  • 1 Borsum T. Biochemical properties of vascular endothelial cells. Virchows Archiv B Cell Pathol 1991; 60: 279-286
  • 2 Cotran RS, Pober JS. Effects of cytokines on vascular endothelium: Their roles in vascular and immune injury. Kidney Int 1989; 35: 969-975
  • 3 Pober JS. Cytokine activation of vascular endothelium: physiology and pathology. Am J Pathol 1988; 113: 426-433
  • 4 Bevilacqua MP. Endothelial-leukocyte adhesion molecules. Annu Rev Immunol 1993; 11: 767-804
  • 5 Springer TA. Adhesion receptors of the immune system. Nature 1990; 346: 425-434
  • 6 Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 1993; 362: 801-809
  • 7 Aqel NM, Ball RY, Waldmann H, Mitchinson MJ. Monocytic origin of foam cells in human atherosclerotic plaques. Atherosclerosis 1984; 53: 265-271
  • 8 Newman W, Beall LD, Carson CW. et al Soluble E-selectin is found in supernatants of activated endothelial cells and is elevated in the serum of patients with septic shock. J Immunol 1993; 150: 644-654
  • 9 Gearing AJH, Hemingway I, Pigott R, Hughes J, Rees AJ, Cashman SJ. Soluble forms of vascular adhesion molecules, E-selectin, ICAM-1, VCAM-1: Pathological significance. Annals NY Acad Sci 1992; 667: 324-331
  • 10 Haming R, Mainolfi E, Bystryn JC, Henn M, Merluzzi VJ, Rothlein R. Serum levels of circulating intercellular adhesion molecule 1 in human malignant melanoma. Cancer Res 1991; 51: 5003-5005
  • 11 Shijubo N, Imai K, Aoki S. et al Circulating intercellular adhesion molecule-1 (ICAM-1) antigen in sera of patients with idiopathic pulmonary fibrosis. Clin Exp Immunol 1992; 89: 58-62
  • 12 Gearing AJH, Newman W. Circulating adhesion molecules in disease. Immunol Today 1993; 14: 506-512
  • 13 Blann AD, McCollum CN. von Willebrand factor, endothelial cell damage and atherosclerosis. Eur J Vase Surg 1994; 8: 10-15
  • 14 Wagner DD. Cell biology of von Willebrand factor. Ann Rev Cell Biology 1990; 6: 217-246
  • 15 Blann AD. Normal levels of von Willebrand factor antigen in human body fluids. Biologicals 1990; 18: 351-353
  • 16 Adams DH, Mainolfi E, Elias E, Neuberger JK, Rothlein R. Detection of circulating ICAM-1 after liver transplantation: evidence for local release within the liver during allograft rejection. Transplantation 1992; 55: 83-87
  • 17 Ballantyne CM, Mainolfi EA, Young JB. et al Prognostic value of increased levels of circulating ICAM-1 after heart transplant. Clin Res 1991; 39: 285
  • 18 Norton J, Sloane JP, Al-Saffar N, Haskard DO. Expression of adhesion molecules in human intestinal graft versus host disease. Clin Exp Immunol 1992; 87: 231-236
  • 19 Rice EG, Bevilacqua MP. An inducible endothelial cell surface glycoprotein mediates melanoma adhesion. Science 1989; 246: 1303-1306
  • 20 Pigott R, Dillon LP, Hemingway IH, Gearing AJ. Soluble forms of E-selectin, ICAM-1 and VCAM-1 are present in the supernatants of cytokine activated endothelial cells. Biochem Biophys Res Comm 1992; 187: 584-589
  • 21 Giddings JC, Shall L. Enhanced release of von Willebrand factor by human endothelial cells in culture in the presence of phorbol myristate acetate and interleukin-1. ThrombRes 1987; 47: 259-267
  • 22 Kaul A, Blake DR, Pearson JD. Vascular endothelium, cytokines and the pathogenesis of inflammatory synovitis. Annals Rheum Dis 1991; 50: 828-832
  • 23 Blann AD. von Willebrand factor as a marker of injury to the endothelium in inflammatory vascular disease. J Rheum 1993; 20: 1469-1471
  • 24 Anonymous Editorial. Atherosclerosis goes to the wall. Lancet 1992; 339: 647-648
  • 25 Li H, Cybulsky MI, Gimbrone MA, Libby P. An atherogenic diet rapidly induces VCAM-1, a cytokine regulatable mononuclear leukocyte adhesion molecule, in rabbit aortic endothelium. Arteriosclerosis Thromb 1993; 13: 197-204
  • 26 Marlin SD, Staunton DE, Springer TA, Stratowa C, Sommergruber W, Merluzzi VJ. A soluble form of intercellular adhesion molecule-1 inhibits rhinovirus infection. Nature 1990; 344: 70-72
  • 27 Becker JC, Dummer R, Hartmann AA, Burg G, Schmidt RE. Shedding of ICAM-1 from human melanoma cell lines induced by IFN-gamma and tumour necrosis factoralpha. Functional consequences on cell mediated cytotoxicity. J Immunol 1991; 147: 4398-4401
  • 28 Couffinhal T, Duplaa C, Labat L. et al Tumour necrosis factor-alpha stimulates ICAM-1 expression in human vascular smooth muscle cells. Arteriosclerosis Thromb 1993; 13: 407-414