A Photometric Assay for Blood Coagulation Factor XIII

Summary

An assay for a direct photometric determination of F XIII in untreated and undiluted plasma was developed. In a one-step procedure F XIII is activated by thrombin and Ca²⁺ and crosslinks glycine-ethylester to a specific glutamine containing peptide substrate. The released ammonia is incorporated into α-keto-glutarate by glutamate dehydrogenase, and the NADH consumption of this reaction is measured photometrically at 340 nm. NADH-consumption is directly proportional to the F XIII activity. Fibrin polymerization and the corresponding turbidity is avoided by the use of a fibrin aggregation inhibitor.

The procedure is rapid and simple and enables to measure within the range of 0 to 150% F XIII. It can be performed with automated analyzers as well as with common photometric equipment.

The normal range of F XIII activity in 167 healthy donors was determined to be 70 to 140%.

• References

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