Thromb Haemost 1991; 65(04): 421-424
DOI: 10.1055/s-0038-1648164
Original Article
Schattauer GmbH Stuttgart

Superoxide Dismutase Cooperates with Prostacyclin to Inhibit Platelet Aggregation: a Comparative Study in Washed Platelets and Platelet Rich Plasma

Daniela Salvemini
The William Harvey Research Institute, St. Bartholomew’s Hospital Medical College, London, United Kingdom
,
Gilberto de Nucci
The William Harvey Research Institute, St. Bartholomew’s Hospital Medical College, London, United Kingdom
,
John R Vane
The William Harvey Research Institute, St. Bartholomew’s Hospital Medical College, London, United Kingdom
› Author Affiliations
Further Information

Publication History

Received: 23 April 1990

Accepted after revision 27 November 1990

Publication Date:
02 July 2018 (online)

Summary

The role of superoxide anions (O2 ) in human platelet aggregation in Krebs’ buffer or plasma was investigated. In indome thacin (10 μM)-treated washed platelets superoxide dismutase (SOD; 60 U/ml) or ferricytochrome c (FCC; 70 μM) inhibited platelet aggregation by thrombin but not that by collagen or ADP. In addition, in indomethacin (10 μM)-treated washed platelets, SOD significantly potentiated the anti-aggregatory activity of prostacyclin (PGI2) or iloprost when thrombin but not collagen was used as the aggregating agent. In platelet rich plasma, SOD (60 U/ml) did not inhibit platelet aggregation nor did it potentiate the anti-aggregatory activity of iloprost when ADP, collagen or thrombin were used as aggregating agents. Thus, O2 participate in the aggregatory activity of thrombin but not collagen or ADP and PGI2 or iloprost, by reducing the sensitivity of platelets to thrombin, co-operate with SOD to inhibit thrombin-induced platelet aggregation

The interpretation of the use of SOD in experiments involving endothelium-derived relaxing factor (NO) is discussed

 
  • References

  • 1 Marcus AJ. Pathways of oxygen utilization by stimulated platelets and leukocytes. Semin Haematol 1979; 16: 188-195
  • 2 Joseph M, Capron A, Tsicopoulos A, Ameisen JC, Martinot JB, Tonnel AB. Platelet activation by IgE and aspirin. Agents Actions 1987; 21: 169-177
  • 3 Cesbron JY, Capron A, Vargaftig BB, Lagarde M, Pincemail T, Braquet P, Taelman H, Joseph M. Platelets mediate the action of diethylcarbamazine on microfilariae. Nature 1987; 325: 533-536
  • 4 Henry RL. Leukocytes and thrombosis. Thromb Diath Haemorrh 1965; 13: 35-46
  • 5 McCord JM, Salin ML. Free radicals and inflammation: studies on superoxide-mediated NBT reduction by leukocytes. In: Erythrocytes Structure and Function Brewer GJ. (ed). Alan R. Liss, Inc.,; New York: 1975. pp 731-752
  • 6 Gryglewski RJ, Palmer RMJ, Moncada S. Superoxide anion is involved in the breakdown of endothelium-derived vascular relaxing factor. Nature 1986; 320: 454-456
  • 7 Rubanyi GM, Vanhoutte PM. Superoxide anions and hyperoxia inactivate endothelium-derived relaxing factor. Am J Physiol 1986; 250: H215-221
  • 8 Radomski MW, Palmer RMJ, Moncada S. Endogenous nitric oxide inhibits human platelet adhesion to vascular endothelium. Lancet 1987; ii: 1057-1058
  • 9 Sneddon JM, Vane JR. Endothelium-derived relaxing factor reduces platelet adhesion to bovine endothelial cells. Proc Natl Acad Sci USA 1988; 85: 2800-2804
  • 10 Azuma H, Ishikawa M, Sekizaki S. Endothelium-dependent inhibition of platelet aggregation. Br J Pharmacol 1986; 88: 411-415
  • 11 Radomski MW, Palmer RMJ, Moncada S. Comparative pharmacology of endothelium-derived relaxing factor, nitric oxide and prostacyclin in platelets. Br J Pharmacol 1987; 92: 181-187
  • 12 Salvemini D, de Nucci G, Sneddon JM, Vane JR. Superoxide anions enhance platelet adhesion and aggregation. Br J Pharmacol 1989; 97: 1145-1150
  • 13 Handin RI, Karabin R, Boxer GJ. Enhancement of platelet function by superoxide anion. J Clin Invest 1976; 59: 959-965
  • 14 Radomski MW, Moncada S. An improved method for washing human platelets with prostacyclin. Thromb Res 1983; 30: 383-389
  • 15 Born GVR, Cross MJ. The aggregation of blood platelets. J Physiol 1963; 168: 178-195
  • 16 McCord JM, Fridovich L. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem 1969 244. 6049-6055
  • 17 Frei B, Stocker R, Ames BN. Anti-oxidant defences and lipid peroxidation in human blood plasma. Proc Natl Acad Sci USA 1988; 85: 9748-9752
  • 18 Krinsky NI, Sladdin D, Levine PH, Taub IA, Simic MG. Modification of platelet function by radical species produced during irradiation of oxygenated water. Thromb Haemostas 1981; 45: 116-120
  • 19 Matsubara T, Ziff M. Superoxide anion release by human endothelial cells: synergism between a phorbolester and a calcium ionophore. J Cell Physiol 1986; 127: 207-210
  • 20 Cockcroft S. Phosphoinositides and neutrophil activation. In: Phosphoinositides and Receptor Mechanisms Putney Jr JW. (ed). Alan R. Liss, Inc.; New York: 1986. pp 287-310
  • 21 Martin W, Villani GM, Jothianandan D, Furchgott RF. Selective blockade of endothelium-dependent and glyceryl trinitrate-induced relaxation by hemoglobin and by methylene blue in the rabbit aorta. J Pharmacol Exp Ther 1985; 232: 708-716