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DOI: 10.1055/s-0038-1647919
Platelet Adhesion, Release and Aggregation in Flowing Blood: Effects of Surface Properties and Platelet Function.[*]
Publication History
Received:
06 August 1975
accepted:
06 August 1975
Publication Date:
02 July 2018 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/197601/lookinside/thumbnails/10-1055-s-0038-1647919-1.jpg)
Summary
Platelet adhesion to natural and artificial surfaces and adhesion-induced aggregation were investigated in vitro using an annular perfusion chamber. The surfaces were exposed to anticoagulated blood under identical flow conditions (~ arterial shear rates). The initial attachment of platelets (contact) appeared less surface specific than spreading and release. Fibrillar collagen was the most powerful inducer of platelet degranulation whereas elastin, microfibrils and epon were virtually inactive. Fibrillar collagen caused release also in the absence of spreading. Surface coverage with platelets did not exceed 25 % unless spreading occurred. Perfusion with platelet-free plasma or platelet-poor blood did not remove adhering platelets. However, platelets were translocated from mural thrombi to the surface by such perfusion. In addition, platelets which detached from mural thrombi adhered more readily to elastin or microfibrils than platelets from the circulating blood. The initial attachment of platelets to subendothelium was inhibited in von Willebrand’s disease, the Bernard-Soulier syndrome and at high concentrations of dipyridamole; spreading was inhibited in storage pool disease of rats, at low temperature (20° C), with EDTA (3 mM) and Prostaglandin E1 (1 μM); and adhesion-induced aggregation was inhibited in thrombasthenia, storage pool disease and after ingestion of sulfinpyrazone or Aspirin.
It is concluded that the initial attachment (contact) of platelets, spreading and surface-induced release of platelet constituents are at least partially independent phenomena, the latter two being highly surface specific. At flow conditions which cause the disappearance of platelet thrombi, platelet adhesion appears as an irreversible process.
* Presented at the Vth Congress of the International Society on Thrombosis and Haemostasis, Paris, July, 1975.
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Reference
- 1 Baumgartner H. R. 1972; Platelet interaction with vascular structures. Thrombosis et Diathesis Haemorrhagica Supplementum 51: 161.
- 2 Baumgartner H. R. 1973; The role of blood flow in platelet adhésion, fibrin déposition and formation of mural thrombi. Microvascular Research 5: 167.
- 3 Baumgartner H. R. 1974; a The subendothelial surface and thrombosis. Thrombosis et Diathesis Haemorrhagica Supplementum 59: 91.
- 4 Baumgartner H. R. 1974; b Morphometric quantitation of adherence of platelets to an artificial surface and components of connective tissue. Thrombosis et Diathesis Haemorrhagica Supplementum 60: 39.
- 5 Baumgartner H. R, Haudenschild C. 1972; Adhesion of platelets to subendothelium. Annals of the New York Academy of Sciences 201: 22.
- 6 Baumgartner H. R, Stemerman M. B, Spaet T. H. 1971; Adhesion of blood platelets to subendothelial surface: distinct from adhesion to collagen. Experientia 27: 283.
- 7 Baumgartner H. R, Tranzer J. P, Studer A. 1967; An electron microscopic study of platelet thrombus formation in the rabbit with particular regard to 5 – hydroxy tryptamine release. Thrombosis et Diathesis Haemorrhagica 18: 592.
- 8 Brass L. F, Bensusan H. B. 1974; Role of collagen quaternary structure in platelet-collagen interaction. Journal of Clinical Investigation 54: 1480.
- 9 Breddin K. 1964; Zur Messung der Thrombozytenadhäsivität. Thrombosis et Diathesis Haemorrhagica 12: 269.
- 10 Cazenave J.-P, Packham M. A, Mustard J. F. 1973; Adherence of platelets to a collagen-coated surface: development of a quantitative method. Journal of Laboratory and Clinical Medicine 82: 978.
- 11 Friedman L. I, Leonard E. F. 1971; Platelet adhesion to artificial surfaces: consequences of flow, exposure time, blood condition, and surface nature. Federation Proceedings 30: 1641.
- 12 Goldsmith H. L. 1972. The flow of model particles and blood cells and its relation to thrombo-genesis. In: Spaet T. H. (ed.), Progress in Hemostasis and Thrombosis, Volume 1.. Grune and Stratton; New York: 97.
- 13 Hellem A. J. 1960 The adhesiveness of human blood platelets in vitro.. Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum 51.
- 14 Hovig T. 1963; Aggregation of rabbit blood platelets produced in vitro by saline “extract” of tendons. Thrombosis et Diathesis Haemorrhagica 9: 248.
- 15 Jenkins C. S. P, Packham M. A, Guccione M. A, Mustard J. F. 1973; Modification of platelet adherence to protein-coated surfaces. Journal of Laboratory and Clinical Medicine 81: 280.
- 16 Leonard E. F, Friedman L. I. 1970; Thrombogenesis on artificial surfaces: a flow reactor problem. Chemical Engineering Progress Symposium Series 66: 59.
- 17 Leonard E. F, Grabowski E. F, Turitto V. T. 1972; The role of convection and diffusion on platelet adhesion and aggregation. Annals of the New York Academy of Sciences 201: 329.
- 18 Majno G, Palade G. E. 1961; Studies on inflammation. I. The effect of histamine and serotonin on vascular permeability. Journal of Biophysical and Biochemical Cytology 11: 571.
- 19 Muggli R, Baumgartner H. R. 1975; Collagen coated gelatine tubes for the investigation of platelet adhesion and subsequent platelet aggregation under controlled flow conditions. Thrombosis et Diathesis Haemorrhagica 34: 333.
- 20 Mustard J. F, Kinlough-Rathbone R. L, Packham M. A. 1974; Recent status of research in the pathogenesis of thrombosis. Thrombosis et Diathesis Haemorrhagica Supplementum 59: 157.
- 21 Pletscher A, Da Prada M, Berneis K. H, Tranzer J. P. 1971; New aspects on the storage of 5-hydroxytryptamine in blood platelets. Experientia 27: 993.
- 22 Ross R, Bornstein P. 1969; The elastic fiber. I. The separation and partial characterization of its macromolecular components. Journal of Cell Biology 40: 366.
- 23 Stemerman M. B, Baumgartner H. R, Spaet T. H. 1971; The subendothelial microfibril and platelet adhesion. Laboratory Investigation 24: 170.
- 24 Tranzer J. P, Baumgartner H. R. 1967; Filling gaps in the vascular endothelium with blood platelets. Nature 216: 1126.
- 25 Tranzer J. P, Da Prada M, Pletscher A. 1966; Ultrastructural localization of 5-hydroxy-tryptamine in blood platelets. Nature 212: 1574.
- 26 Tschopp T. B, Baumgartner H. R. 1975; Delayed adhesion to subendothelium and defective aggregation of platelets from rats with storage pool disease (SPD). Thrombosis et Diathesis Haemorrhagica 34: 680.
- 27 Tschopp T. B, Weiss H. J, Baumgartner H. R. 1974; Decreased adhesion of platelets to subendothelium in von Willebrand’s disease. Journal of Laboratory and Clinical Medicine 83: 296.
- 28 Tschopp T. B, Weiss H. J, Baumgartner H. R. 1975; Interaction of thrombasthenic platelets with subendothelium: normal adhesion, absent aggregation. Experientia 31: 113.
- 29 Turttto V. T. 1975; Mass transfer in annuli under conditions of laminar flow. Chemical Engineering Science 30: 503.
- 30 Turitto V. T, Baumgartner H. R. 1974; a Effect of physical factors on platelet adherence to subendothelium. Thrombosis et Diathesis Haemorrhagica Supplementum 60: 17.
- 31 Turitto V. T, Baumgartner H. R. 1974; b Effect of temperature on platelet interaction with subendothelium exposed to flowing blood. Haemostasis 3: 224.
- 32 Turitto V. T, Baumgartner H. R. 1975; Platelet interaction with subendothelium in a perfusion system: physical role of red blood cells. Microvascular Research 9: 335.
- 33 Weiss H. J, Tschopp T. B, Baumgartner H. R, Sussman I. I, Johnson M. M, Egan J. J. 1974; Decreased adhesion of giant (Bernard-Soulier) platelets to subendothelium. Further implications on the role of the von Willebrand factor in hemostasis. American Journal of Medicine 57: 920.
- 34 Weiss H. J, Tschopp T. B, Baumgartner H. R. 1975; Impaired interaction (adhesion-aggregation) of platelets with the subendothelium in storage pool disease and after aspirin ingestion-a comparison with von Willebrand’s disease. New England Journal of Medicine 293: 619.
- 35 Zucker M. B, Borrelli J. 1962; Platelet clumping produced by connective tissue suspensions and by collagen. Proceedings of the Society of Experimental Biology and Medicine 109: 779.