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Thromb Haemost 1990; 64(02): 319-325
DOI: 10.1055/s-0038-1647309
Original Article
Schattauer GmbH Stuttgart

Identification of Dense Granule Specific Membrane Proteins in Bovine Platelets that Are Absent in the Chediak-Higashi Syndrome

Kenneth Meyers
The Department of Veterinary and Comparative Anatomy Pharmacology and Physiology Washington State University, Pullman, WA, USA
,
Carrie Seachord
The Department of Veterinary and Comparative Anatomy Pharmacology and Physiology Washington State University, Pullman, WA, USA
› Author Affiliations
Further Information

Publication History

Received 08 September 1989

Accepted after revision02 May 1990

Publication Date:
25 July 2018 (online)

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Summary

Platelets from cattle with the Chediak-Higashi syndrome (CHS) have a platelet dense granule deficiency. One hypothesis for the platelet dense granule deficiency is that the granule is simply not formed in CHS megakaryocytes (MK). Alternative hypotheses include that the granule is assembled in CHS MK but a functional amino-nucleotide-cation storage complex cannot be formed or that the dense granule or its precursor fuses with other granules. This study was undertaken to determine if membrane proteins specific for platelet dense granules can be identified in membranes of other granules in CHS platelets. Platelets were disrupted; a mixed-granule fraction and alpha-granule enriched, mitochondrial-enriched, and dense granule-enriched subfractions were obtained. Membrane proteins in these intact granules were radiolabeled and the granule underwent hypotonic lysis. Membrane proteins were extracted from granule “ghosts”, separated, and then visualized by autoradiography. Three major proteins were identified in platelet dense granule membrane subfractions. Two of these proteins could be identified in membrane extracts from the mixed-granule fraction from normal platelets. They could neither be identified in extracts from the mixed granule fraction of CHS platelets nor in membranes from alpha granule-enriched and mitochondrial-enriched subfractions. The absence of dense granule membrane proteins in membranes of other organelles within CHS platelets suggests that fusion of dense granules or its precursor with other granules cannot account for the platelet dense granule deficiency in CHS platelets.

 

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