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Thromb Haemost 1990; 64(02): 211-215
DOI: 10.1055/s-0038-1647287
Original Article
Schattauer GmbH Stuttgart

The Association of Platelet and Red Cell Count with Platelet Impedance Changes in Whole Blood and Light-Scattering Changes in Platelet Rich Plasma: Evidence from the Caerphilly Collaborative Heart Disease Study

Dan S Sharp
1   The Medical Research Council Epidemiology Unit, Cardiff, United Kingdom
,
Andrew D Beswick
1   The Medical Research Council Epidemiology Unit, Cardiff, United Kingdom
,
John R O'Brien
2   Central Laboratory, Saint Mary’s Hospital, Portsmouth, United Kingdom
,
Serge Renaud
3   The INSERM Unit 63, Bron, France
,
John W G Yarnell
1   The Medical Research Council Epidemiology Unit, Cardiff, United Kingdom
,
Peter C Elwood
1   The Medical Research Council Epidemiology Unit, Cardiff, United Kingdom
› Author Affiliations
Further Information

Publication History

Received 06 September 1989

Accepted after revision 07 May 1990

Publication Date:
25 July 2018 (online)

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Summary

This epidemiological study was undertaken to explore possible relationships among various haematological indices, prevalent ischaemic heart disease and platelet “function” as measured by two rather different methods. ADP-induced platelet impedance changes in whole blood were strongly associated with prevalent ischaemic heart disease in a general population of 49-66 year men at increased risk. Adenosine diphosphate (ADP) induced platelet aggregation in platelet rich plasma (PRP) at a constant platelet count and also the whole blood platelet count and red cell (RBC) count were strongly and independently related to ADP-induced platelet impedance changes. Both platelet count and platelet aggregation in PRP assessed by changes in optical density were directly related to increasing platelet “sensitivity” as measured by impedance changes in whole blood but RBC count was inversely related. Positive independent relationships between platelet impedance changes and plasma viscosity and fibrinogen were markedly attenuated when platelet count was taken into account, but this finding does not discount a role for these factors in platelet aggregation. No relationship was noted between white blood cell (WBC) count and platelet impedance changes; however, a significant inverse relationship was noted with platelet aggregation in PRP. These findings indicate that laboratory-based experimental findings can be observed in population based studies, and that these haematological factors may be important indicators of ischaemic disease in the population.

 

  • References

  • 1 Born G VR. Aggregation of blood platelets by adenosine disphos-phate and its reversal. Nature 1962; 194: 927-929
    PubMedGoogle Scholar
  • 2 O’Brien JR. Platelet aggregation: II. Some results from a new method of study. J Clin Pathol 1962; 15: 452-455
    CrossrefPubMedGoogle Scholar
  • 3 Gear A RL. Rapid reactions of platelets studied by a quenched-flow approach: aggregation kinetics. J Lab Clin Med 1982; 100: 866-886
    PubMedGoogle Scholar
  • 4 Frojmovic MM, Milton JG, Gear A RL. Platelet aggregation measured in vitro by microscopic and electronic particle counting. Methods Enzymol 1989; 169: 134-149
    PubMedGoogle Scholar
  • 5 Cardinal DC, Flower RJ. The electronic aggregometer: a novel device for assessing platelet behavior in blood. J Pharmacol Methods 1980; 3: 135-158
    CrossrefPubMedGoogle Scholar
  • 6 Gresele P, Zoja C, Deckmyn H, Amout J, Vermylen J, Verstraete M. Dipyridamole inhibits platelet aggregation in whole blood. Thromb Haemostas 1983; 50: 852-856
    PubMedGoogle Scholar
  • 7 Ingerman-Wojenski CM, Silver MJ. A quick method for screening platelet dysfunctions using the whole blood lumi-aggregometer. Thromb Haemostas 1984; 51: 154-156
    PubMedGoogle Scholar
  • 8 Abbate R, Favilla S, Boddi M, Costanzo G, Prisco D. Factors influencing platelet aggregation in whole blood. Am J Clin Pathol 1986; 86: 91-96
    CrossrefPubMedGoogle Scholar
  • 9 Harrison M JG, Emmons PR, Mitchell J RA. The effect of white cells on platelet aggregation. Thromb Diath Haemorr 1966; 16: 105-121
    PubMedGoogle Scholar
  • 10 Machi J, Sigel B, Ramos JR, Justin JR, Feinberg H, Le Breton GC, Robertson AL. Role of red cells in preventing the growth of platelet aggregation. Thromb Res 1984; 36: 53-66
    CrossrefPubMedGoogle Scholar
  • 11 Harrison M JG, Pollock SS, Weisblatt E. Haematocrit and platelet aggregation. Lancet 1984; 2: 991-992
    PubMedGoogle Scholar
  • 12 Gordge MP, Dodd NJ, Rylance PB, Weston MJ. An assessment of whole blood impedance aggregometry using blood from normal subjects and haemodialysis patients. Thromb Res 1984; 36: 17-27
    CrossrefPubMedGoogle Scholar
  • 13 Elwood PC, Beswick AD, Sharp DS, Yamell J WG, Rogers S, Renaud S. Whole blood impedance platelet aggregation and ischemic heart disease: The Caerphilly Collaborative Heart Disease Study. Arteriosclerosis (in press)
    Google Scholar
  • 14 Saniabadi AR, Lowe G DO, Barbenel JC, Forbes CD. Haematocrit, bleeding time, and platelet aggregation. Lancet 1984; 1: 1409-1410
    PubMedGoogle Scholar
  • 15 Saniabadi AR, Lowe G DO, Barbenel JC, Forbes CD. A comparison of spontaneous platelet aggregation in whole blood with platelet rich plasma: additional evidence for the role of ADP. Thromb Haemostas 1984; 51: 115-118
    PubMedGoogle Scholar
  • 16 Caerphilly and Speedwell Collaborative Group. Caerphilly and Speedwell collaborative heart disease studies. J Epidemiol Community Health 1984; 38: 259-262
    CrossrefPubMedGoogle Scholar
  • 17 Renaud S, Dumont E, Godsey F, Suplisson A, Thevenon C. Platelet functions in relation to dietary fats in farmers from two regions of France. Thromb Haemostas 1979; 40: 518-531
    PubMedGoogle Scholar
  • 18 SAS Institute Inc. SAS/STATTM User’s Guide, Release 6.03. SAS Institute Inc; Cary, NC: 1988: 641-666
    Google Scholar
  • 19 Leigh JP. Assessing the importance of an independent variable in multiple regression: is step wise unwise. J Clin Epidemiol 1988; 41: 669-677
    CrossrefPubMedGoogle Scholar
  • 20 Giles C. The platelet count and mean platelet volume. Br J Haematol 1981; 48: 31-37
    PubMedGoogle Scholar
  • 21 Bessman JD, Williams LC, Gilmer PR. Mean platelet volume: The inverse relation between plateled size and count in normal subjects, and an artifact of other particles. Am J Clin Pathol 1981; 76: 189-193
    PubMedGoogle Scholar
  • 22 Levin J, Bessman JD. The inverse relation between platelet volume and platelet number: Abnormalities in hematologic disease and evidence that platelet size does not correlate with platelet age. J Lab Clin Med 1983; 101: 295-307
    PubMedGoogle Scholar
  • 23 Bessman JD, Gilmer PR, Gardner FH. Use of mean platelet volume improves detection of platelet disorders. Blood Cells 1985; 11: 127-135
    PubMedGoogle Scholar
  • 24 Holme S, Simmonds M, Ballek R, Murphy S. Comparative measurements of platelet size by Coulter Counter, microscopy of blood smears, and light-transmission studies: Relationship between platelet size and shape. J Lab Clin Med 1981; 97: 610-622
    PubMedGoogle Scholar
  • 25 Holme S, Murphy S. Influence of platelet count and size on aggregation studies. J Lab Clin Med 1981; 97: 623-630
    PubMedGoogle Scholar
  • 26 Frojmovic MM. Comparative studies of turbidometrically measured rates of platelet aggregation require adjustment of the platelet suspension according to the mean relative size and optical efficiency of the platelets used. Blood 1989; 74: 2302
    PubMedGoogle Scholar
  • 27 Thompson CB, Jakubowski JA, Quinn PG, Deykin D, Valeri CR. Platelet size as a determinant of platelet function. J Lab Clin Med 1983; 101: 205-213
    PubMedGoogle Scholar
  • 28 Wong T, Pedvis L, Frojmovic M. Platelet size affects both micro- and macro-aggregation: contributions of platelet number, volume fraction and cell surface. Thromb Haemostas 1989; 62: 733-741
    PubMedGoogle Scholar
  • 29 Machi J, Sigel B, Ramos JR, Justin JR, Feinberg H, LeBreton GC, Robertson AL. Role of red cells in preventing the growth of platelet aggregation. Thromb Res 1984; 36: 53-66
    CrossrefPubMedGoogle Scholar