Download PDF
Thromb Haemost 1988; 60(02): 314-318
DOI: 10.1055/s-0038-1647052
Original Article
Schattauer GmbH Stuttgart

Dose-Response Aggregometry in Maternal/Neonatal Platelets

A M A Gader
*   The Department of Physiology, College of Medicine & King Khaled University Hospital, Riyadh, Saudi Arabia
,
H Bahakim
**   The Department of Paediatrics, College of Medicine & King Khaled University Hospital, Riyadh, Saudi Arabia
,
F A Jabbar
***   The Department of Obstetrics & Gynaecology, College of Medicine & King Khaled University Hospital, Riyadh, Saudi Arabia
,
A L Lambourne
****   The Department of Community Medicine, College of Medicine & King Khaled University Hospital, Riyadh, Saudi Arabia
,
T H Gaafar
1   The Maternity Hospital, Riyadh, Saudi Arabia
,
Y B Edrees
1   The Maternity Hospital, Riyadh, Saudi Arabia
› Author Affiliations
Further Information

Publication History

Received 11 September 1987

Accepted after revision 01 July 1988

Publication Date:
23 July 2018 (online)

Also available at
  PDF Download  Permissions and Reprints

Summary

The aggregation of platelets collected from maternal/neonatal pairs (n = 240) at the time of childbirth, was studied in response to multiple doses of ADP, collagen, arachidonic acid and ristocetin. Similar responses were obtained from healthy nonpregnant adult controls for comparison. The lag phase, slope of the aggregation curves as well as maximum aggregation (MA%) were recorded and analysed. Neonatal and adult platelets exhibited more enhanced responses to decreasing doses of ADP, arachidonic acid and ristocetin, than maternal platelets. These enhanced responses were exhibited more consistantly in the slopes of the aggregation curves than in MA%. Although neonatal platelets have shown longer lag phase in their responses to collagen, the rate of the aggregation reaction was significantly faster than maternal platelets, with no differences in MA%. These results contradict many previous reports suggesting impaired aggregation responses of neonatal platelets to these agonist. The possible reasons for these contradictions were discussed.

Keywords

Blood platelets - Aggregation - Dose response - Neonates
 

  • References

  • 1 Mull MM, Hathaway WE. Altered platelet function in newborn. Pediat Res 1970; 4: 229-237
    CrossrefPubMedGoogle Scholar
  • 2 Corby DG, Zuck TF. Newborn platelet dysfunction: a storage pool and release defect. Thromb Haemostas 1976; 36: 200-207
    PubMedGoogle Scholar
  • 3 Stuart MJ. The neonatal platelet: Evaluation of platelet malonyldial- dehyde formation as an indicator of prostaglandin synthesis. Br J Haematol 1979; 39: 83-90
    PubMedGoogle Scholar
  • 4 Corby DG, Shulman I. The effect of antenatal drug administration on aggregation of newborn infants. Pediatr Pharmacol Ther 1979; 79: 307-313
    PubMedGoogle Scholar
  • 5 Andrews NP, Broughton PipkinF, Heptinstall S. Blood platelet behaviour in mothers and neonates. Thromb Res 1985; 53: 428-432
    PubMedGoogle Scholar
  • 6 Ts'ao C, Green D, Schultz K. Function and ultrastructure of platelets of neonates: Enhanced ristocetin aggregation of neonatal platelets. Br J Haematol 1976; 32: 225-233
    CrossrefPubMedGoogle Scholar
  • 7 Jones CR, McCabe R, Hamilton CA, Reid JL. Maternal and fetal platelet responses and adrenoceptor binding characteristics. Thromb Haemostas 1985; 53: 95-98
    PubMedGoogle Scholar
  • 8 Bleyer WA, Brechenridge RJ. Adverse blood reactions in the newborn. II prenatal aspirin and newborn hemostasis. JAMA 1970; 213: 2049
    CrossrefPubMedGoogle Scholar
  • 9 Thompson SG, Vickers MV. Methods in dose response platelet aggregometry. Thromb Haemostas 1985; 53: 216-218
    PubMedGoogle Scholar
  • 10 Gader AM A, Bahakim H, Malaika S, Jabbar FA. Haemostatic parameters in various ethnic groups in Saudi Arabia. Thromb Haemostas 1987; 58: 558 (Abstr.)
    PubMedGoogle Scholar
  • 11 Eichelberger JW. Kinetic (slope) measurement of platelet aggregation. Bio Data Corporation; Hatboro, PA 19040: 1984
    Google Scholar
  • 12 O'Brien JR, Heywood JB, Heady JA. The quantitation of platelet aggregation induced by four compounds: a study in relation to myocardial infarction. Thromb Diath Haemorrh 1966; 16: 752-767
    PubMedGoogle Scholar
  • 13 Yamazaki H, Takahaski T, Sano T. Hyperaggregability of platelets in thromboembolic disorders. Thromb Diath Haemorrh 1975; 34: 94-105
    PubMedGoogle Scholar
  • 14 Ts'ao C, Peterson KG, Gulluzzo TS. Opposite changes in aggregability of platelets stored in plasma and in blood. Am J Clin Pathol 1979; 71: 379-383
    CrossrefPubMedGoogle Scholar
  • 15 Olsen JD, Brockway WJ, Fass DN, Magnuson MA, Bowie EJW. Evaluation of ristocetin-Willebrand factor assay and ristocetin induced platelet aggregation. Am J Clin Pathol 1975; 63: 210
    CrossrefPubMedGoogle Scholar
  • 16 Berrettini M, Buoncristiani U, Parise P, Ballatori E, Nenci G. Polyacrilonytril versus cuprophan membranes for hemodialysis: evaluation of efficacy and bicompatibility by platelet aggregation studies. Int J Artificial Organs 1981; 218-222
    PubMedGoogle Scholar
  • 17 Ahlsten G, Ewald U, Tuvemo T. Arachidonic acid-induced aggregation of platelets from human cord blood compared with platelets from adults. Biol Neonate 1985; 47: 199-204
    CrossrefPubMedGoogle Scholar
  • 18 Kinlough-Rathborne RL, Gazenava JP, Packham MA, Mustard JF. Effect of inhibitors of the arachidonic pathway on the release of granule contents from rabbit platelets adherent to collagen. Lab Invest 1980; 42: 28-34
    PubMedGoogle Scholar
  • 19 Corby DG, O'Barr TP. Decreased alpha-adrenergic receptors in newborn platelets: cause of abnormal response to epinephrine. Dev Pharmacol Ther 1981; 2: 215-225
    CrossrefPubMedGoogle Scholar
  • 20 Landolfi R, De CristofaroR, De CarolisS, Ciabattoni G, Bizzi B. Placental-derived PGI2 in the transient hyporeactivity of newborn platelets. Thromb Haemostas 1987; 58: 402 (Abstr)
    PubMedGoogle Scholar