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Thromb Haemost 1978; 39(02): 360-365
DOI: 10.1055/s-0038-1646696
Original Article
Schattauer GmbH Stuttgart

Platelet Metal Levels in Normal Subjects Determined by Atomic Absorption Spectrophotometry

Richmond J Baker
The Division of Nuclear Medicine, Institute of Medical and Veterinary Science, Frome Road, Adelaide S.A. 5000, Australia
,
John J McNeil
The Division of Nuclear Medicine, Institute of Medical and Veterinary Science, Frome Road, Adelaide S.A. 5000, Australia
,
Harry Lander
The Division of Nuclear Medicine, Institute of Medical and Veterinary Science, Frome Road, Adelaide S.A. 5000, Australia
› Author Affiliations
Further Information

Publication History

Received 16 March 1977

Accepted 20 October 1977

Publication Date:
12 July 2018 (online)

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Summary

A method has been developed for the estimation of iron, copper, zinc, calcium and magnesium in human platelets using atomic absorption spectrophotometry. Fe and Cu are either absent or present in very low concentration approaching the limit of detection: Zn, Ca and Mg are readily detectable. EDTA is preferable to ACD(A) or heparin as the anticoagulant for collection in that the platelet yield is reliable: however, slightly low results for Ca and Mg are obtained. Corrections for residual plasma and contaminating red cells are essential. Plasma and platelet metal levels in 33 normal subjects are reported together with the findings in four studies undertaken on platelets obtained from subjects with polycythemia vera.

 

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