Thromb Haemost 1989; 61(03): 397-401
DOI: 10.1055/s-0038-1646603
Original Article
Schattauer GmbH Stuttgart

The Action of a Synthetic Pentasaccharide on Thrombin Generation in Whole Plasma

S Béguin
The Department of Biochemistry University of Limburg, Maastricht, The Netherlands
,
J Choay
*   Institut Choay, Paris, France
,
H C Hemker
The Department of Biochemistry University of Limburg, Maastricht, The Netherlands
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Received 22. November 1988

Accepted after revision 22. Februar 1989

Publikationsdatum:
24. Juli 2018 (online)

Summary

We investigated the effect on thrombin generation in plasma of the pentasaccharide that represent the AT II/binding site in heparin. This compound has no effect on the breakdown of thrombin in plasma. It dose-dependently inhibits the formation of thrombin in both the intrinsic and the extrinsic pathway. If coagulation is triggered by the complete prothrombinase complex (phospholipid – factor Va – factor Xa) under conditions in which the large majority of factor Xa is bound to the complex, the inhibition of prothrombinase activity is only minor. If no factor Va is present or if the prothrombinase activity is triggered by adding complete tenase (PL-FVIIIa-FIXa) or incomplete tenase (PLFIXa) to the plasma the inhibition by pentasaccharide is of the same magnitude as that in the intrinsic or extrinsic system.

We conclude that the pentasaccharide inhibits blood coagulation by katalysing the inactivation of free factor Xa. In contrast to classical heparin it does inhibit the peak of thrombin formation in platelet rich plasma, probably because it is less subject to inactivation by heparin binding proteins from platelets than classical heparin is.

 
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