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Thromb Haemost 1989; 61(01): 015-019
DOI: 10.1055/s-0038-1646519
Original Article
Schattauer GmbH Stuttgart

ARIC[*]Hemostasis Study - I. Development of a Blood Collection and Processing System Suitable for Multicenter Hemostatic Studies

A C Papp
1   The Vascular Disease Research Center, University of Texas Health Science at Houston, Houston, Texas, USA
2   The Division of Hematology/Oncology, University of Texas Health Science at Houston, Houston, Texas, USA
,
H Hatzakis
1   The Vascular Disease Research Center, University of Texas Health Science at Houston, Houston, Texas, USA
2   The Division of Hematology/Oncology, University of Texas Health Science at Houston, Houston, Texas, USA
,
A Bracey
3   The Departments of Medicine and Pathology, University of Texas Health Science at Houston, Houston, Texas, USA
,
K K Wu
1   The Vascular Disease Research Center, University of Texas Health Science at Houston, Houston, Texas, USA
2   The Division of Hematology/Oncology, University of Texas Health Science at Houston, Houston, Texas, USA
3   The Departments of Medicine and Pathology, University of Texas Health Science at Houston, Houston, Texas, USA
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Publikationsverlauf

Received 28. Juli 1988

Accepted after revision 21. Oktober 1988

Publikationsdatum:
24. Juli 2018 (online)

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Summary

In order to carry out a multicenter study aimed at understanding the association of hemostatic factors with atherosclerotic vascular disorders for the Atherosclerosis Risk In Communities (ARIC) Study, we compared a blood collection and processing system developed in our laboratory with the state-of-the-art- procedures. The salient features of our system included the use of a new phlebotomy set for venipuncture, the use of Millipore filters for removing platelet residues in the plasma and the use of a mixture of anticoagulants and antiplatelet agents for inhibiting the in vitro activation of platelets, coagulation and fibrinolytic system. The results derived from systematic evaluations indicate that this newly developed system yields the lowest values of plasma ßTG, PF 4 and FPA when compared with the reported values. The technique also gave reliable values of representative hemostatic measurements such as fibrinogen, factor VII, factor VIII, von Willebrand factor, antithrombin-III, protein C, tissue- type plasminogen activator, and serum thromboxane B2. Further experiments revealed that the samples withstood temporary storage at −70° C and overnight “shipping” manipulations without significant changes in the hemostatic values. We conclude that the described blood collection and processing system may be a valuable asset for conducting multicenter cooperative clinical trials and epidemiologic studies involving blood collection by multiple field centers or clinics.

Key words

Blood collecting system - Blood processing system - Multicenter study

* ARIC is the acronym for Atherosclerosis Risk In Communities. It is a prospective epidemiologic study involving 4 diversified U. S. Communities Central laboratories and Coordinating Center. A major component of the ARIC study is to determine the association between hemostatic factors and atherosclerosis and its clinical sequelae. This paper is the first of a series of paper pertaining to hemostasis studies.