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Thromb Haemost 1991; 66(03): 315-320
DOI: 10.1055/s-0038-1646414
Review Article
Schattauer GmbH Stuttgart

Molecular Cloning, Characterization and Expression of cDNA for Rabbit Brain Tissue Factor

Aruna Pawashe
1   The Cardiovascular Division, Department of Medicine, Yale University School of Medicine, New Haven, CT, USA
,
Michael Ezekowitz
1   The Cardiovascular Division, Department of Medicine, Yale University School of Medicine, New Haven, CT, USA
,
Tsung-Chung Lin
2   The Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT, USA
,
Renata Horton
2   The Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT, USA
,
Ronald Bach
3   The Division of Thrombosis Research, Department of Medicine, Mount Sinai Medical School, City University of New York, New York, NY, USA
,
William Konigsberg
2   The Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT, USA
› Author Affiliations
Further Information

Publication History

Received 08 January 1991

Accepted 14 March 1991

Publication Date:
25 July 2018 (online)

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Summary

Tissue factor (TF) is a membrane anchored glycoprotein that initiates blood coagulation by forming a complex with circulating factor VII or VIIa. TF has been identified in atherosclerotic plaques and may possibly trigger thrombosis after spontaneous plaque rupture as seen in acute myocardial infarction or angioplasty. We have previously developed an atherosclerotic rabbit model for study of the acute and chronic outcomes following angioplasty. As a first step in developing inhibitors of TF, we have isolated and characterized a rabbit cDNA coding for the mature TF. The sequence comparison of rabbit TF cDNA with those of human and mouse TFs show considerable similarity at both the nucleotide and amino acid levels. The TF cDNA when expressed in E. coli demonstrates a procoagulant activity comparable to that of native rabbit brain TF. The TF activity can be blocked by a polyclonal antibody against rabbit TF.

 

  • References

  • 1 Bach R. Initiation of coagulation by tissue factor. CRC Crit Rev Biochem 1988; 23: 339-68
    PubMedGoogle Scholar
  • 2 Nemerson Y. Tissue factor and hemostasis. Blood 1988; 71: 1-8
    PubMedGoogle Scholar
  • 3 Drake TA, Morrissey JM, Edgington TS. Selective cellular expression of tissue factor in human tissues. Implications for disorders of hemostasis and thrombosis. Am J Pathol 1989; 134: 1087-97
    PubMedGoogle Scholar
  • 4 Wilcox JN, Smith KM, Schwartz S, Gordon D. Localization of tissue factor in the normal vessel wall and in the atherosclerotic plaque. Proc Natl Acad Sci USA 1989; 86: 2839-43
    CrossrefPubMedGoogle Scholar
  • 5 Sarembock IJ, LaVeau PJ, Sigal SL, Timms I, Sussman J, Haudenschild C, Ezekowitz MD. Influence of inflation pressure and balloon size on the development of intimal hyperplasia after balloon angioplasty. A study in the atherosclerotic rabbit. Circulation 1989; 80: 1029-40
    CrossrefPubMedGoogle Scholar
  • 6 LaVeau PJ, Sarembock IJ, Sigal SJ, Yang TL, Ezekowitz MD. Vascular reactivity after balloon angioplasty in an atherosclerotic rabbit. Circulation 1990; 82: 1790-801
    CrossrefPubMedGoogle Scholar
  • 7 Steele PM, Chesebro JH, Stanson AW, Holmes Jr DR, Dewanjee MK, Badimon L, Fuster V. Balloon angioplasty. Natural history of the patho-physiological response to injury in a pig model. Circ Res 1985; 57: 105-12
    CrossrefPubMedGoogle Scholar
  • 8 Wilentz JR, Sanborn TA, Haudenschild CC, Valeri CR, Ryan TJ, Faxon DP. Platelet accumulation in experimental angioplasty: time course and relation to vascular injury. Circulation 1987; 75: 636-42
    CrossrefPubMedGoogle Scholar
  • 9 Gruentzig A. Results from coronary angioplasty and implications for future. Am Heart J 1982; 103: 779-83
    CrossrefPubMedGoogle Scholar
  • 10 Holmes DR, Vlietstra RE, Smith HC, Vetrovec GW, Kent KM, Cowley MJ, Faxon DP, Gruentzig AR, Kelsey SF, Detre KM, Van Raden MJ, Mock MB. Restenosis after percutaneous transluminal coronary angioplasty (PTCA): A report from PTCA registry of the National Heart, Lung and Blood Institute. Am J Cardiol 1984; 53: 77c-81c
    CrossrefPubMedGoogle Scholar
  • 11 Spicer EK, Horton R, Bloem L, Bach R, Williams KR, Guha A, Kraus J, Lin T-C, Nemerson Y, Konigsberg WK. Isolation of cDNA clones coding for human tissue factor: Primary structure of the protein and cDNA. Proc Natl Acad Sci USA 1987; 84: 5148-52
    CrossrefPubMedGoogle Scholar
  • 12 Hartzell S, Ryder K, Lanhan A, Lau LF, Nathans D. A growth factor-responsive gene of murine BALB/c 3T3 cells encodes a protein homologous to human tissue factor. Mol Cell Biol 1989; 9: 2567-73
    CrossrefPubMedGoogle Scholar
  • 13 Saiki RK, Walsh PS, Levenson CH, Erlich HA. Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes. Proc Natl Acad Sci USA 1989; 86: 6230-4
    CrossrefPubMedGoogle Scholar
  • 14 Maniatis T, Fritzch EF, Sambrook J. Molecular Cloning, a Laboratory Manual. Cold Spring Harbor Laboratory: 1989
    Google Scholar
  • 15 Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA. 1977; 74: 5463-7
    CrossrefPubMedGoogle Scholar
  • 16 Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned gene. Mol Biol 1986; 189: 113-30
    PubMedGoogle Scholar
  • 17 Rao LVM, Hoang AD. Purification and characterization of rabbit tissue factor. Thromb Res 1989; 56: 109-18
    CrossrefPubMedGoogle Scholar
  • 18 Bradford M. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-54
    CrossrefPubMedGoogle Scholar
  • 19 Bloem LJ, Chen L, Konigsberg WH, Bach R. Serum stimulation of quiescent human fibroblasts induces the synthesis of tissue factor mRNA followed by the appearance of tissue factor antigen and procoagulant activity. J Cell Physiol 1989; 139: 418-23
    CrossrefPubMedGoogle Scholar
  • 20 Carson SD. Cadmium causes vesicle leakage under conditions which favour reconstitution of tissue factor-vesicle complexes. J Membrane Biol 1983; 75: 123-7
    CrossrefPubMedGoogle Scholar
  • 21 Carson SD, Konigsberg WH. Cadmium increases tissue factor (coagulation factor III) activity by facilitating its reassociation with lipids. Science 1980; 208: 307-9
    CrossrefPubMedGoogle Scholar
  • 22 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 1970; 227: 680-5
    CrossrefPubMedGoogle Scholar
  • 23 Sim GK, Yague J, Nelson J, Marrack P, Palmer E, Augustin A, Kappler J. Primary structure of human T-cell receptor α-chain. Nature 1984; 312: 771-5
    CrossrefPubMedGoogle Scholar
  • 24 Fisher KL, Gorman CM, Vehar GA, O'Brien DP, Lawn RM. Cloning and expression of human tissue factor. Thromb Res 1987; 48: 89-99
    CrossrefPubMedGoogle Scholar
  • 25 Morrissey JM, Fakhrai H, Edgington TS. Molecular cloning of the cDNA for tissue factor, the cellular receptor for the initiation of the coagulation protease cascade. Cell 1987; 50: 129-35
    CrossrefPubMedGoogle Scholar
  • 26 Scarpati EM, Wen D, Broze Jr GJ, Miletich JP, Flandermeyer RR, Siegel NR, Sadler JE. Human tissue factor: cDNA sequence and chromosome localization of the gene. Biochemistry 1987; 26: 5234-8
    CrossrefPubMedGoogle Scholar
  • 27 Janson TL, Stormorken H, Prydz H. Species specificity of tissue thromboplastin. Haemostasis 1984; 14: 440-4
    PubMedGoogle Scholar
  • 28 Bach R, Konigsberg WH, Nemerson Y. Human tissue factor contains thioester-linked palmitate and stearate on the cytoplasmic half-cystine. Biochemistry 1988; 27: 4227-31
    CrossrefPubMedGoogle Scholar
  • 29 Mcllhinney RAJ. The fats of life: the importance and function of protein acylation. Trends Biochem Sci 1990; 15: 387-91
    CrossrefPubMedGoogle Scholar
  • 30 Paborsky LR, Tate KM, Harris RJ, Yansura DG, Band L, MaCray G, Gorman CM, O'Brien DP, Chang JY, Swartz JR, Fung VP, Thomas JN, Vehar GA. Purification of recombinant human tissue factor. Biochemistry 1989; 28: 8072-7
    CrossrefPubMedGoogle Scholar
  • 31 Dalbey RE. Positively charged residues are important determinants of membrane protein topology. Trends Biochem Sci 1990; 15: 253-7
    CrossrefPubMedGoogle Scholar
  • 32 Bazan JF. Structural design and molecular evolution of a cytokine receptor superfamily. Proc Natl Acad Sci USA 1990; 87: 6934-8
    CrossrefPubMedGoogle Scholar
  • 33 Carlsen E, Flatmark A, Prydz H. Cytokine-induced procoagulant activity in monocytes and endothelial cells. Further enhancement by cyclosporine. Transplantation 1988; 46: 575-80
    CrossrefPubMedGoogle Scholar
  • 34 Goualt-Hellmann M, Chardon E, Sultan E, Josso F. The procoagulant factor of leukaemeic promyelocytes: demonstration of immunological cross-reactivity with human brain tissue factor. Br J Haematol 1975; 30: 151-8
    CrossrefPubMedGoogle Scholar