Thromb Haemost 1991; 66(03): 315-320
DOI: 10.1055/s-0038-1646414
Review Article
Schattauer GmbH Stuttgart

Molecular Cloning, Characterization and Expression of cDNA for Rabbit Brain Tissue Factor

Aruna Pawashe
1  The Cardiovascular Division, Department of Medicine, Yale University School of Medicine, New Haven, CT, USA
,
Michael Ezekowitz
1  The Cardiovascular Division, Department of Medicine, Yale University School of Medicine, New Haven, CT, USA
,
Tsung-Chung Lin
2  The Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT, USA
,
Renata Horton
2  The Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT, USA
,
Ronald Bach
3  The Division of Thrombosis Research, Department of Medicine, Mount Sinai Medical School, City University of New York, New York, NY, USA
,
William Konigsberg
2  The Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT, USA
› Author Affiliations
Further Information

Publication History

Received 08 January 1991

Accepted 14 March 1991

Publication Date:
25 July 2018 (online)

Summary

Tissue factor (TF) is a membrane anchored glycoprotein that initiates blood coagulation by forming a complex with circulating factor VII or VIIa. TF has been identified in atherosclerotic plaques and may possibly trigger thrombosis after spontaneous plaque rupture as seen in acute myocardial infarction or angioplasty. We have previously developed an atherosclerotic rabbit model for study of the acute and chronic outcomes following angioplasty. As a first step in developing inhibitors of TF, we have isolated and characterized a rabbit cDNA coding for the mature TF. The sequence comparison of rabbit TF cDNA with those of human and mouse TFs show considerable similarity at both the nucleotide and amino acid levels. The TF cDNA when expressed in E. coli demonstrates a procoagulant activity comparable to that of native rabbit brain TF. The TF activity can be blocked by a polyclonal antibody against rabbit TF.