Download PDF
Thromb Haemost 1993; 70(01): 087-093
DOI: 10.1055/s-0038-1646166
State-of-the-Art Lecture
Plateley Receptors
Schattauer GmbH Stuttgart

Platelet Integrins[*]

Mark H Ginsberg
Committee on Vascular Biology, The Scripps Research Institute, La Jolla, CA, USA
,
Du Xiaoping
Committee on Vascular Biology, The Scripps Research Institute, La Jolla, CA, USA
,
Timothy E O'Toole
Committee on Vascular Biology, The Scripps Research Institute, La Jolla, CA, USA
,
Joseph C Loftus
Committee on Vascular Biology, The Scripps Research Institute, La Jolla, CA, USA
,
Edward F Plow
Committee on Vascular Biology, The Scripps Research Institute, La Jolla, CA, USA
› Author Affiliations
Further Information

Publication History

Publication Date:
03 July 2018 (online)

  PDF Download  Permissions and Reprints

* Publication # 7945-CVB from the Scripps Research Institute


 

  • References

  • 1 Ginsberg MH, Pierschbacher MD, Ruoslahti E, Marguerie GA, Plow EF. Inhibition of fibronectin binding to platelets by proteolytic fragments and synthetic peptides which support fibroblast adhesion. J Biol Chem 1985; 260: 3931-3936
    PubMedGoogle Scholar
  • 2 Pytela RP, Pierschbacher MD, Ginsberg MH, Plow EF, Ruoslahti E. Platelet membrane glycoprotein Ilb/IIIa: Member of a family of Arg-Gly-Asp-specific adhesion receptors. Science 1986; 231: 1559-1562
    CrossrefPubMedGoogle Scholar
  • 3 Plow EF, Loftus J, Levin E, Fair D, Dixon D, Forsyth J, Ginsberg MH. Immunologic relationship between platelet membrane glycoprotein GPIIb/IIIa and cell surface molecules expressed by a variety of cells. Proc Natl Acad Sci USA 1986; 83: 6002-6006
    CrossrefPubMedGoogle Scholar
  • 4 Ginsberg MH, Loftus JC, Ryckwaert J-J, Pierschbacher MD, Pytela R, Ruoslahti E, Plow EF. Immunochemical and amino-terminal sequence comparison of two cytoadhesins indicates that they contain similar or identical beta subunits and distinct alpha subunits. J Biol Chem 1987; 262: 5437-5440
    PubMedGoogle Scholar
  • 5 Hynes RO. Integrins: A family of cell surface receptors. Cell 1987; 48: 549-554
    CrossrefPubMedGoogle Scholar
  • 6 O'Toole TE, Loftus JC, Plow EF, Glass A, Harper JR, Ginsberg MH. Efficient surface expression of platelet GPIIb-IIIa requires both subunits. Blood 1989; 74: 14-18
    PubMedGoogle Scholar
  • 7 Hynes RO. Integrins: Versatility, modulation, and signalling in cell adhesion. Cell 1992; 69: 11-25
    CrossrefPubMedGoogle Scholar
  • 8 Ginsberg MH, Loftus JC, Plow EF. Cytoadhesins, integrins, and platelets. Thromb Haemostas 1988; 59: 1-6
    PubMedGoogle Scholar
  • 9 van Kuppevelt TH, Languino LR, Gailit JO, Suzuki S, Ruoslahti E. An alternative cytoplasmic domain of the integrin beta 3 subunit. Proc Natl Acad Sci USA 1989; 86: 5415-5418
    CrossrefPubMedGoogle Scholar
  • 10 Altruda F, Cervella P, Tarone G, Botta C, Balzac F, Stefanuto G, Silengo L. A human integrin beta 1 subunit with a unique cytoplasmic domain generated by alternative mRNA processing. Gene 1990; 95: 261-266
    CrossrefPubMedGoogle Scholar
  • 11 Languino LR, Ruoslahti E. An alternative form of the integrin beta 1 subunit with a variant cytoplasmic domain. J Biol Chem 1992; 267: 7116-7120
    PubMedGoogle Scholar
  • 12 Bray PF, Leung CS, Shuman MA. Human platelets and megakaryocytes contain alternately spliced glycoprotein IIb mRNAs. J Biol Chem 1990; 265: 9587-9590
    PubMedGoogle Scholar
  • 13 Hogervorst F, Kuikman I, van Kessel AG, Sonnenberg A. Molecular cloning of the human alpha 6 integrin subunit. Alternative splicing of alpha 6 mRNA and chromosomal localization of the alpha 6 and beta 4 genes. Eur J Biochem 1991; 199: 425-433
    CrossrefPubMedGoogle Scholar
  • 14 Solowska J, Guan J-L, Marcantonio EE, Trevithick JE, Buck CA, Hynes RO. Expression of normal and mutant avian integrin subunits in rodent cells. J Cell Biol 1989; 109: 853-861
    CrossrefPubMedGoogle Scholar
  • 15 O'Toole TE, Mandelman D, Forsyth J, Shattil SJ, Plow EF, Ginsberg MH. Modulation of the affinity of integrin αIIbβ3 (GPIIb-IIIa) by the cytoplasmic domain of αIIb . Science 1991; 254: 845-847
    CrossrefPubMedGoogle Scholar
  • 16 Kolodziej MA, Vilaire G, Rifat S, Poncz M, Bennett JS. Effect of deletion of glycoprotein IIb Exon 28 on the expression of the platelet glycoprotein Ilb/IIIa complex. Blood 1992; 78: 2344-2353
    PubMedGoogle Scholar
  • 17 Nurden AT, Caen JP. An abnormal platelet glycoprotein pattern in three cases of Glanzmann's thrombasthenia. Br J Haematol 1974; 28: 253-260
    CrossrefPubMedGoogle Scholar
  • 18 Jennings LK, Phillips DR. Purification of glycoproteins IIb and III from human platelet plasma membranes and characterization of a calcium-dependent glycoprotein Ilb-IIIa complex. J Biol Chem 1982; 257: 10458-10466
    PubMedGoogle Scholar
  • 19 Fitzgerald LA, Steiner B, Rall Jr. SC, Lo SS, Phillips DR. Protein sequence of endothelial glycoprotein IIIa derived from a cDNA clone. Identity with platelet glycoprotein IIIa and similarity to “integrin”. J Biol Chem 1987; 262: 3936-3939
    PubMedGoogle Scholar
  • 20 Loftus JC, Plow EF, Frelinger III AL, D'Souza SE, Dixon D, Lacy J, Sorge J, Ginsberg MH. Molecular cloning and chemical synthesis of a region of platelet GPIIb involved in adhesive function. Proc Natl Acad Sci USA 1987; 840: 7114-7118
    PubMedGoogle Scholar
  • 21 Poncz M, Eisman R, Heidenreich R, Silver SM, Vilaire G, Surrey S, Schwartz E, Bennett JS. Structure of the platelet membrane glycoprotein IIb. Homology to the alpha subunits of the vitronectin and fibronectin membrane receptors. J Biol Chem 1987; 262: 8476-8482
    PubMedGoogle Scholar
  • 22 Rosa J-P, Bray PF, Gayet O, Johnston GI, Cook RG, Jackson KW, Shuman MA, McEver RP. Cloning of glycoprotein IIIa cDNA from human erythroleukemia cells and localization of the gene to chromosome 17. Blood 1988; 72: 593-600
    PubMedGoogle Scholar
  • 23 Gailit J, Ruoslahti E. Regulation of the fibronectin receptor affinity by divalent cations. J Biol Chem 1988; 263: 12927-12932
    PubMedGoogle Scholar
  • 24 Staatz WD, Rajpara SM, Wayner EA, Carter WG, Santoro SA. The membrane glycoprotein Ia-IIa (VLA-2) complex mediates the Mg++-dependent adhesion of platelets to collagen. J Cell Biol 1989; 108: 1917-1924
    CrossrefPubMedGoogle Scholar
  • 25 Marlin SD, Springer TA. Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1). Cell 1987; 51: 813-819
    CrossrefPubMedGoogle Scholar
  • 26 Bennett JS, Vilaire G. Exposure of platelet fibrinogen receptors by ADP and epinephrine. J Clin Invest 1979; 64: 1393-1401
    CrossrefPubMedGoogle Scholar
  • 27 Phillips DR, Baughan AK. Fibrinogen binding to human platelet plasma membranes. Identification of two steps requiring divalent cations. J Biol Chem 1983; 258: 10240-10246
    PubMedGoogle Scholar
  • 28 Steiner B, Cousot D, Trzeciak A, Gillessen D, Hadvary P. Ca2+-dependent binding of a synthetic Arg-Gly-Asp (RGD) peptide to a single site on the purified platelet glycoprotein Ilb-IIIa complex. J Biol Chem 1989; 264: 13102-13108
    PubMedGoogle Scholar
  • 29 Ginsberg MH, Lightsey A, Kunicki TJ, Kaufman A, Marguerie GA, Plow EF. Divalent cation regulation of the surface orientation of platelet membrane glycoprotein IIb: Correlation with fibrinogen binding function and definition of a novel variant of Glanzmann's thrombasthenia. J Clin Invest 1986; 78: 1103-1111
    CrossrefPubMedGoogle Scholar
  • 30 Frelinger III AL, Lam SC-T, Plow EF, Smith MA, Loftus JC, Ginsberg MH. Occupancy of an adhesive glycoprotein receptor modulates expression of an antigenic site involved in cell adhesion. J Biol Chem 1988; 263: 12397-12402
    PubMedGoogle Scholar
  • 31 Ginsberg MH, Frelinger III AL, Lam SC-T, Forsyth J, McMillan R, Plow EF, Shattil SJ. Analysis of platelet aggregation disorders based on flow cytometric analysis of membrane glycoprotein Ilb-IIIa with conformation-specific monoclonal antibodies. Blood 1990; 76: 2017-2023
    PubMedGoogle Scholar
  • 32 Loftus JC, O'Toole TE, Plow EF, Glass A, Frelinger III AL, Ginsberg MH. A β3 integrin mutation abolishes ligand binding and alters divalent cation-dependent conformation. Science 1990; 249: 915-918
    CrossrefPubMedGoogle Scholar
  • 33 D'Souza SE, Ginsberg MH, Burke TA, Lam SC-T, Plow EF. Localization of an Arg-Gly-Asp recognition site within an integrin adhesion receptor. Science 1988; 242: 91-93
    CrossrefPubMedGoogle Scholar
  • 34 MacKrell AJ, Blumberg B, Haynes SR, Fessler JH. The lethal myospheroid gene of Drosophila encodes a membrane protein homologous to vertebrate integrin beta subunits. Proc Natl Acad Sci USA 1988; 85: 2633-2637
    CrossrefPubMedGoogle Scholar
  • 35 Kretsinger RH. Structure and evolution of calcium-modulated proteins. CRC Crit Rev Biochem 1980; 8: 119-174
    PubMedGoogle Scholar
  • 36 Ruoslahti E, Pierschbacher MD. Arg-Gly-Asp: A versatile cell recognition signal. Cell 1986; 44: 517-518
    CrossrefPubMedGoogle Scholar
  • 37 Kloczewiak M, Timmons S, Lukas TJ, Hawiger J. Platelet receptor recognition site on human fibrinogen. Synthesis and structure-function relationship of peptides corresponding to the carboxy-terminal segment of the gamma chain. Biochemistry 1984; 23: 1767-1774
    CrossrefPubMedGoogle Scholar
  • 38 Wayner EA, Garcia-Pardo A, Humphries MJ, McDonald JA, Carter WG. Identification and characterization of the T lymphocyte adhesion receptor for an alternative cell attachment domain (CS-1) in plasma fibronectin. J Cell Biol 1989; 109: 1321-1330
    CrossrefPubMedGoogle Scholar
  • 39 Staatz WD, Fok KF, Zutter MM, Adams SP, Rodriguez BA, Santoro SA. Identification of a tetrapeptide recognition sequence for the alpha-2 beta-1 integrin in collagen. J Biol Chem 1991; 266 (12) 7363-7367
    PubMedGoogle Scholar
  • 40 Ruggeri ZM, Houghten RA, Russell SR, Zimmerman TS. Inhibition of platelet function with synthetic peptides designed to be high-affinity antagonists of fibrinogen binding to platelets. Proc Natl Acad Sci USA 1986; 83: 5708-5712
    CrossrefPubMedGoogle Scholar
  • 41 Takada Y, Ylanne J, Mandelman D, Puzon W, Ginsberg MH. A point mutation of integrin β3 subunit blocks binding of α5β1 to fibronectin and invasin but not recruitment to adhesion plaques. J Cell Biol 1992; 119: 913-921
    CrossrefPubMedGoogle Scholar
  • 42 Smith JW, Cheresh DA. The Arg-Gly-Asp binding domain of the vitronectin receptor. Photoaffinity cross-linking implicates amino acid residues 61-203 of the β subunit. J Biol Chem 1988; 263: 18726-18731
    PubMedGoogle Scholar
  • 43 Andrieux A, Rabiet MJ, Chapel A, Concord E, Marguerie G. A highly conserved sequence of the Arg-Gly-Asp-binding domain of the integrin beta 3 subunit is sensitive to stimulation. J Biol Chem 1991; 266: 14202-14207
    PubMedGoogle Scholar
  • 44 Bajt ML, Loftus JC, Gawaz MP, Ginsberg MH. Characterization of a gain of function mutation of integrin αIIbβ3 (platelet GPIIb-IIIa). J Biol Chem 1992; 267: 22211-22216
    PubMedGoogle Scholar
  • 45 Lam SC-T, Plow EF, Smith MA, Andrieux A, Ryckwaert J-J, Marguerie GA, Ginsberg MH. Evidence that Arginyl-Glycyl-Aspartate peptides and fibrinogen gamma chain peptides share a common binding site on platelets. J Biol Chem 1987; 262: 947-950
    PubMedGoogle Scholar
  • 46 Lam SC-T, Plow EF, D'Souza SE, Cheresh DA, Frelinger III AL, Ginsberg MH. Isolation and characterization of a platelet membrane protein related to the vitronectin receptor. J Biol Chem 1989; 264: 3742-3749
    PubMedGoogle Scholar
  • 47 Cheresh DA, Berliner SA, Vicente V, Ruggeri ZM. Recognition of distinct adhesive sites on fibrinogen by related integrins on platelets and endothelial cells. Cell 1989; 58: 945-953
    CrossrefPubMedGoogle Scholar
  • 48 D'Souza SE, Ginsberg MH, Burke TA, Plow EF. The ligand binding site of the platelet integrin receptor GPIIb-IIIa is proximal to the second calcium binding domain of its alpha subunit. J Biol Chem 1990; 265: 3440-3446
    PubMedGoogle Scholar
  • 49 D'Souza SE, Ginsberg MH, Matsueda GR, Plow EF. A discrete sequence in a platelet integrin is involved in ligand recognition. Nature 1991; 350: 66-68
    CrossrefPubMedGoogle Scholar
  • 50 Taylor DB, Gartner TK. A peptide corresponding to GPIIbα 300-312, a presumptive fibrinogen gamma-chain binding site on the platelet integrin GPIIb/IIIa, inhibits the adhesion of platelets to at least four adhesive ligands. J Biol Chem 1992; 267: 11729-11733
    PubMedGoogle Scholar
  • 51 Charo IF, Nannizzi L, Phillips DR, Hsu MA, Scarborough RM. Inhibition of fibrinogen binding to GPIIb-IIIa by a GP IIIa peptide. J Biol Chem 1991; 266: 1414-1421
    PubMedGoogle Scholar
  • 52 Bajt ML, Ginsberg MH, Frelinger III AL, Berndt MC, Loftus JC. A spontaneous mutation of integrin αIIbβ3 (platelet GPIIb-IIIa) helps define a ligand binding site. J Biol Chem 1992; 267: 3789-3794
    PubMedGoogle Scholar
  • 53 Lanza F, Stierle A, Fournier D, Morales M, Andre G, Nurden AT, Cazenave J. A new variant of Glanzmann's thrombasthenia (Strasbourg I). J Clin Invest 1992; 89: 1995-2004
    CrossrefPubMedGoogle Scholar
  • 54 Calvete JJ, Arias J, Alvarez MV, Lopez MM, Henschen A, Gonzalez-Rodriguez J. Further studies on the topography of the N-terminal region of human platelet glycoprotein IIIa. Localization of monoclonal antibody epitopes and the putative fibrinogen-binding sites. Biochem J 1991; 274: 457-463
    CrossrefPubMedGoogle Scholar
  • 55 Gartner TK, Loudon R, Taylor DB. The peptides APLHK, EHIPA and GAPL are hydropathically equivalent peptide mimics of a fibrinogen binding domain of glycoprotein Ilb/IIIa. Biochem Biophys Res Commun 1991; 180: 1446-1452
    CrossrefPubMedGoogle Scholar
  • 56 Calvete JJ, Arias J, Alvarez MV, Lopez MM, Henschen A, Gonzalez-Rodriguez J. Further studies on the topography of human platelet glycoprotein IIb. Biochem J 1991; 273: 767-775
    CrossrefPubMedGoogle Scholar
  • 57 Sims PJ, Ginsberg MH, Plow EF, Shattil SJ. Effect of platelet activation on the conformation of the plasma membrane glycoprotein IIb-IIIa complex. J Biol Chem 1991; 266: 7345-7352
    PubMedGoogle Scholar
  • 58 O'Toole TE, Loftus JC, Du X, Glass AA, Ruggeri ZM, Shattil SJ, Plow EF, Ginsberg MH. Affinity modulation of the αIIbβ3 integrin (platelet GPIIb-IIIa) is an intrinsic property of the receptor. Cell Regul 1990; 1: 883-893
    CrossrefPubMedGoogle Scholar
  • 59 Peerschke EI, Zucker MB, Grant RA, Egan JJ, Johnson MM. Correlation between fibrinogen binding to human platelets and platelet aggregability. Blood 1980; 55: 841-847
    PubMedGoogle Scholar
  • 60 Dustin ML, Springer TA. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Nature 1989; 341: 619-624
    CrossrefPubMedGoogle Scholar
  • 61 Fujimoto T, Ohara S, Hawiger J. Thrombin-induced exposure and prostacyclin inhibition of the receptor for factor VIII/von Willebrand factor on human platelets. J Clin Invest 1982; 69: 1212-1222
    CrossrefPubMedGoogle Scholar
  • 62 Shattil SJ, Brass LF. Induction of the fibrinogen receptor on human platelets by intracellular mediators. J Biol Chem 1987; 262: 992-1000
    PubMedGoogle Scholar
  • 63 Shattil SJ, Cunningham M, Wiedmer T, Zhao J, Sims PJ, Brass LF. Regulation of glycoprotein Ilb-IIIa receptor function studied with platelets permeabilized by the pore-forming complement proteins C5b-9. J Biol Chem 1992; 267: 18424-18431
    PubMedGoogle Scholar
  • 64 Hillery CA, Smyth SS, Parise LV. Phosphorylation of human platelet glycoprotein IIIa (GPIIIa). J Biol Chem 1991; 266: 14663-14669
    PubMedGoogle Scholar
  • 65 Nemoto Y, Namba T, Teru-uchi T, Ushikubi F, Morii N. A rho gene product in human blood platelets. J Biol Chem 1992; 267: 20916-20920
    PubMedGoogle Scholar
  • 66 Morii N, Teru-uchi T, Tominaga T, Kumagai N, Kozaki S, Ushikubi F, Narumiya S. A rho gene product in human blood platelets. II. Effects of ADP-ribosylation by botulinum C3 ADP-ribosyltransferase on platelet aggregation. J Biol Chem 1992; 267: 20921-20926
    PubMedGoogle Scholar
  • 67 Ridley AJ, Hall A. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell 1992; 70: 389-399
    CrossrefPubMedGoogle Scholar
  • 68 Marcantonio EE, Hynes RO. Antibodies to the conserved cytoplasmic domain of the integrin β1 subunit react with proteins in vertebrates, invertebrates, and fungi. J Cell Biol 1988; 106: 1765-1772
    CrossrefPubMedGoogle Scholar
  • 69 Takada Y, Murphy E, Pil P, Chen C, Ginsberg MH, Hemler ME. Molecular cloning and expression of the cDNA for alpha3 subunit of human alpha3beta1 (VLA-3), an integrin receptor for fibronectin, laminin and collagen. J Cell Biol 1991; 115: 257-266
    CrossrefPubMedGoogle Scholar
  • 70 Hemler ME. VLA proteins in the integrin family: Structures, functions, and their role on leukocytes. Annu Rev Immunol 1990; 8: 365-400
    CrossrefPubMedGoogle Scholar
  • 71 Tamura RN, Cooper HM, Collo G, Quaranta V. Cell type-specific integrin variants with alternative alpha chain cytoplasmic domains. Proc Natl Acad Sci USA 1991; 88: 10183-10187
    CrossrefPubMedGoogle Scholar
  • 72 Chen YP, Djaffar I, Pidard D, Steiner B, Cieutat AM, Caen JP, Rosa JP. Ser-752 - Pro mutation in the cytoplasmic domain of integrin β3 subunit and defective activation of platelet integrin αIIbβ3 (glycoprotein Ilb-IIIa) in a variant of Glanzmann thrombasthenia. Proc Natl Acad Sci USA 1992; 89: 10169-10173
    CrossrefPubMedGoogle Scholar
  • 73 Brass LF, Manning DR, Williams AG, Woolkalis MJ, Poncz M. Receptor and G protein-mediated responses to thrombin in HEL cells. J Biol Chem 1991; 266: 958-965
    PubMedGoogle Scholar
  • 74 Larson RS, Hibbs ML, Springer TA. The leukocyte integrin LFA-1 reconstituted by cDNA transfection in a nonhematopoietic cell line is functionally active and not transiently regulated. Cell Regul 1990; 1: 359-367
    CrossrefPubMedGoogle Scholar
  • 75 Wayner EA, Carter WG, Piotrowicz RS, Kunicki TJ. The function of multiple extracellular matrix receptors in mediating cell adhesion to extracellular matrix: Preparation of monoclonal antibodies to the fibronectin receptor that specifically inhibit cell adhesion to fibronectin and react with platelet glycoproteins Ic-IIa. J Cell Biol 1988; 107: 1881-1891
    CrossrefPubMedGoogle Scholar
  • 76 Ill CR, Engvall E, Ruoslahti E. Adhesion of platelets to laminin in the absence of activation. J Cell Biol 1985; 99: 2140-2145
    PubMedGoogle Scholar
  • 77 Shimizu Y, VanSeventer GA, Horgan KJ, Shaw S. Regulated expression and binding of three VLA (β1) integrin receptors on T cells. Nature 1990; 345: 250-253
    CrossrefPubMedGoogle Scholar
  • 78 Shaw LM, Messier JM, Mercurio AM. The activation dependent adhesion of macrophages to laminin involves cytoskeletal anchoring and phosphorylation of the alpha 6 beta 1 integrin. J Cell Biol 1990; 110: 2167-2174
    CrossrefPubMedGoogle Scholar
  • 79 Du X, Plow EF, Frelinger III AL, O'Toole TE, Loftus JC, Ginsberg MH. Ligands “activate” integrin αIIbβ3 (platelet GPIIb-IIIa). Cell 1991; 65: 409-416
    CrossrefPubMedGoogle Scholar
  • 80 Smyth S, Hillery CA, Parise LV. Fibrinogen binding to purified platelet glycoprotein Ilb-IIIa (Integrin αIIbβ3) is modulated by lipids. J Biol Chem 1992; 267: 15568-15577
    PubMedGoogle Scholar
  • 81 Frelinger III AL, Du X, Plow EF, Ginsberg MH. Monoclonal antibodies to ligand-occupied conformers of integrin αIIbβ3 (Glycoprotein Ilb-IIIa) alters receptor affinity, specificity, and function. J Biol Chem 1991; 266: 17106-17111
    PubMedGoogle Scholar
  • 82 Neugebauer KM, Reichardt LF. Cell-surface regulation of Bl integrin activity on developing retinal neurons. Nature 1991; 350: 68-71
    CrossrefPubMedGoogle Scholar
  • 83 Kovach NL, Carlos TM, Yee E, Harlan JM. A monoclonal antibody to β1 integrin (CD29) stimulates VLA-dependent adherence of leukocytes to human umbilical vein endothelial cells and matrix components. J Cell Biol 1992; 116: 499-509
    CrossrefPubMedGoogle Scholar
  • 84 van de Wiel-van Kemenade E, van Kooyk Y, de Boer AJ, Huijbens RJF, Weder P, van de Kasteele W, Melief CJM, Figdor CG. Adhesion of T and B lymphocytes to extracellular matrix and endothelial cells can be regulated through the beta subunit of VLA. J Cell Biol 1992; 117: 461-470
    CrossrefPubMedGoogle Scholar
  • 85 Faull RJ, Kovach NL, Harlan JM, Ginsberg MH. Affinity modulation of integrin α5β1: regulation of the functional response by soluble fibronectin. J Cell Biol. 1993 (in press)
    PubMedGoogle Scholar
  • 86 Coller BS. Interaction of normal, thrombasthenic, and Bernard-Soulier platelets with immobilized fibrinogen: Defective platelet-fibrinogen interaction in thrombasthenia. Blood 1980; 55: 169-178
    PubMedGoogle Scholar
  • 87 Bodary SC, Napier MA, McLean JW. Expression of recombinant platelet glycoprotein IIbIIIa results in a functional fibrinogen-binding complex. J Biol Chem 1989; 264: 18859-18862
    PubMedGoogle Scholar
  • 88 Kieffer N, Fitzgerald LA, Wolf D, Cheresh DA, Phillips DR. Adhesive properties of the β3 integrins: comparison of GPIIb-IIIa and the vitronectin receptor individually expressed in human melanoma cells. J Cell Biol 1991; 113: 451-461
    CrossrefPubMedGoogle Scholar
  • 89 Beer JH, Springer KT, Coller BS. Immobilized Arg-Gly-Asp (RGD) peptides of varying lengths as structural probes of the platelet glycoprotein IIb/IIIa receptor. Blood 1992; 79: 117-128
    PubMedGoogle Scholar
  • 90 Savage B, Marzec UM, Chao BH, Harker LA, Maraganore JM, Ruggeri ZM. Binding of the snake venom-derived proteins applaggin and echistatin to the arginine-glycine-aspartic acid recognition site(s) on platelet glycoprotein IIb.IIIa complex inhibits receptor function. J Biol Chem 1990; 265: 11766-11772
    PubMedGoogle Scholar
  • 91 Adler M, Lazarus RA, Dennis MS, Wagner G. Solution structure of Kistrin, a potent platelet aggregation inhibitor and GP IIb-IIIa antagonist. Science 1991; 253: 445-448
    CrossrefPubMedGoogle Scholar
  • 92 Zamarron C, Ginsberg MH, Plow EF. Monoclonal antibodies specific for a conformationally altered state of fibrinogen. Thromb Haemostas 1990; 64: 41-46
    PubMedGoogle Scholar
  • 93 Zamarron C, Ginsberg MH, Plow EF. A receptor-induced binding site in fibrinogen elicited by its interaction with platelet membrane glycoprotein Ilb-IIIa. J Biol Chem 1991; 266: 16193-16199
    PubMedGoogle Scholar
  • 94 Gawaz MP, Loftus JC, Bajt ML, Frojmovic MM, Plow EF, Ginsberg MH. Ligand bridging mediates integrin αIIbβ3 (Platelet GPIIb-IIIa) dependent homotypic and heterotypic cell-cell interactions. J Clin Invest 1991; 88: 1128-1134
    CrossrefPubMedGoogle Scholar
  • 95 Conforti G, Zanetti A, Pasquali-Ronchetti I, Quaglino Jr. D, Neyroz P, Dejana E. Modulation of vitronectin receptor binding by membrane lipid composition. J Biol Chem 1990; 265: 4011-4019
    PubMedGoogle Scholar
  • 96 Hermanowski-Vosatka A, Van Strijp JAG, Swiggard WJ, Wright SD. Integrin modulating factor-1: a lipid that alters the function of leukocyte integrins. Cell 1992; 68: 341-352
    CrossrefPubMedGoogle Scholar
  • 97 Coller BS, Seligsohn U, West SM, Scudder LE, Norton KJ. Platelet fibrinogen and vitronectin in Glanzmann thrombasthenia: evidence consistent with specific roles for glycoprotein Ilb/IIIa and alpha v beta 3 integrins in platelet protein trafficking. Blood 1991; 78: 2603-2610
    PubMedGoogle Scholar
  • 98 Kunicki TJ, Nugent DJ, Staats SJ, Orchekowski RP, Wayner EA, Carter WG. The human fibroblast class II extracellular matrix receptor mediates platelet adhesion to collagen and is identical to the platelet glycoprotein Ia-IIa complex. J Biol Chem 1988; 263: 4516-4519
    PubMedGoogle Scholar
  • 99 Sonnenberg A, Modderman PW, Hogervorst F. Laminin receptor on platelets is the integrin VLA-6. Nature 1988; 336: 487-489
    CrossrefPubMedGoogle Scholar
  • 100 Piotrowicz RS, Orchekowski RP, Nugent DJ, Yamada KY, Kunicki TJ. Glycoprotein Ic-IIa functions as an activation-independent fibronectin receptor on human platelets. J Cell Biol 1988; 106: 1359-1364
    CrossrefPubMedGoogle Scholar
  • 101 Lipfert L, Haimovich B, Schaller MD, Cobb BS, Parsons JT, Brugge JS. Integrin-dependent phosphorylation and activation of the protein tyrosine kinase ppl25FAK in platelets. J Cell Biol 1992; 119: 905-912
    CrossrefPubMedGoogle Scholar
  • 102 Guan JL, Trevithick JE, Hynes RO. Fibronectin/integrin interaction induces tyrosine phosphorylation of a 120-kDa protein. Cell Regulation 1991; 2: 951-964
    CrossrefPubMedGoogle Scholar
  • 103 Bartfield NS, Kirchofer D, Geltosky JE, Ruoslahti E, Plow EF, Languino LR. Platelets express a unique β1 integrin subunit. Mol Biol Cell 1992; (Suppl. 03) 130a (abstr).
    PubMedGoogle Scholar
  • 104 Calvete JJ, Henschen A, Gonzalez-Rodriguez J. Complete localization of the intrachain disulphide bonds and the N-glycosylation points in the alpha-subunit of human platelet glycoprotein IIb. Biochem J 1989; 261: 561-568
    CrossrefPubMedGoogle Scholar
  • 105 Calvete JJ, Henschen A, Gonzalez-Rodriguez J. Assignment of disulphide bonds in human platelet GPIIIa. A disulphide pattern for the beta-subunits of the integrin family. Biochemical Journal 1991; 274: 63-71
    CrossrefPubMedGoogle Scholar
  • 106 Ginsberg MH, O'Toole TE, Loftus JC, Plow EF. Ligand binding to integrins: Dynamic regulation and common mechanisms. Cold Spring Harbor Symp Quant Biol. 1993 (in press)
    PubMedGoogle Scholar