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Thromb Haemost 1987; 58(04): 960-963
DOI: 10.1055/s-0038-1646036
Original Article
Schattauer GmbH Stuttgart

Selective Staining of Platelet Glycoproteins Using Two-Dimensional O’Farrell Gel Electrophoresis and Avidin-Biotin-Conjugated Lectins

Beate Kehrel
1   The Institute for Arteriosclerosis Research at the University of Münster, University of Berne, Berne, Switzerland
,
Renate Kokott
1   The Institute for Arteriosclerosis Research at the University of Münster, University of Berne, Berne, Switzerland
,
Leopold Balleisen
2   The Department of Internal Medicine, University of Münster, Münster, Federal Republic of Germany
,
Werner Stenzinger
2   The Department of Internal Medicine, University of Münster, Münster, Federal Republic of Germany
,
Kenneth J Clemetson
3   The Theodor Kocher Institute, University of Berne, Berne, Switzerland
,
Jürgen van de Loo
2   The Department of Internal Medicine, University of Münster, Münster, Federal Republic of Germany
› Author Affiliations
Further Information

Publication History

Received 23 October 1987

Accepted after revision 30 June 1987

Publication Date:
29 June 2018 (online)

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Summary

A time-saving and sensitive high resolution method lor the analysis and identification of platelet glycoproteins using non-radioactive compounds has been developed. Platelet proteins (50 μg) of normal subjects were separated by isoelectric focusing and SDS-PAGE. Proteins were either stained with silver or electroblotted onto nitrocellulose. Nitrocellulose blots were treated with the following biotinylated lectins : Abrus precatorius lectin, concanavalin A, Lens culinaris lectin, or wheat germ agglutinin. Staining was achieved by avidin-biotin-coupled peroxidase using 4-chloro-l-naphthol as the substrate. A rapid overview of platelet glycoproteins may be obtained by applying all the lectins to a single blot.

Keywords

Platelet glycoproteins - Two-dimensional O’Farrell gel electrophoresis - Western blot - Avidin-biotin - Lectins
 

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