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Thromb Haemost 1990; 63(01): 072-075
DOI: 10.1055/s-0038-1645689
Original Article
Schattauer GmbH Stuttgart

Different Tissue Plasminogen Activator Release in the Arm and Leg during Venous Occlusion Is Equalized after DDAVP Infusion

Dušan Keber
The Trnovo Hospital of Internal Medicine, University Clinical Center, Ljubljana, Yugoslavia, The Netherlands
,
Mojca Stegnar
The Trnovo Hospital of Internal Medicine, University Clinical Center, Ljubljana, Yugoslavia, The Netherlands
,
Cornelis Kluft
*   The Gaubius Institute, Leiden, The Netherlands
› Author Affiliations
Further Information

Publication History

Received 25 August 1989

Accepted after revision 12 October 1989

Publication Date:
02 July 2018 (online)

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Summary

The mechanism of tissue plasminogen activator (t-PA) release during arm and leg venous occlusions and DDAVP (1 desamino- 8-D-arginine vasopressin) infusion was studied in 10 healthy males. The following determinations were carried out on venous blood: t-PA antigen (ELISA), t-PA activity, and t-PA inhibitor (PAI) activity (amidolytic assays). Before DDAVP, there was a 270% t-PA antigen increase in the arm at the end of occlusion as opposed to only a 40% increase in the leg. After DDAVP, t-PA antigen at the end of arm and leg occlusion reached an equal level which was significantly higher than in the arm before DDAVP. The study produced no evidence of PAI release during venous occlusion of a limb. It is concluded that DDAVP is able to elicit t-PA release from arm as well as from leg vessels. The poor fibrinolytic response of leg vessels to venous occlusion is not due to a high PAT release or t-PA stores depletion in leg vessels, but rather to low basal t-PA release in leg vessels.

Keywords

Tissue plasminogen activator - Venous occlusion - DDAVP - Mechanism of release
 

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