Methodologie Basis for the Radioimmunoassay of Endogenous LH-Like Activity in Human Prostatic Tissue^[*]

The aim of this study was to develop a technique for the radioimmunoiogical determination of the activity of LH-like substances in the human prostate. The material comprised 19 specimens of prostatic tissue obtained during transbladder extirpation in patients with benign prostatic hyperplasia. Tissues of human testes and human sceletal muscle were used as controls. The method adopted for LH extraction from the membrane fraction of human prostatic tissue appeared to be sufficiently specific, accurate and sensitive for routine laboratory investigations. The concentrations of the LH-like immunoreactivity in human testicular tissue was found to be 57, 46 and 70 mU per g of the membrane fraction while those of the prostatic gland tissues ranged from 34 to 155 mU per g of the membrane fraction. However such LH-like substance was not found in human sceletal muscle tissue. It seems that the LH-type activity is an indirect proof for the existence of LH receptors in the human prostate.

Der Zweck dieser Arbeit war die Entwicklung einer Technik der radioimmunologischen Bestimmung der Aktivität LH-ähnlicher Substanzen in der menschlichen Prostata. Das Material wurde von Kranken mit benigner Prostatahypertrophie während der Exstirpation durch die Blase entnommen und bestand aus 19 Probestücken des Prostatagewebes. Proben menschlichen Hodengewebes und menschlichen Skelettmuskelgewebes wurden als Kontrolle verwendet. Die gewählte Methode für die LH-Extraktion aus der Membranfraktion des menschlichen Prostatagewebes hat sich als genügend spezifisch, genau und empfindlich für die Routine-Laboruntersuchungen erwiesen. Die Konzentrationen der LH-ähnlichen Immunoreaktivität des menschlichen Hodengewebes betrugen 57, 46 und 70 mU pro g der Membranfraktion, während die Konzentrationen des Prostatadrüsengewebes von 34 bis 155 mU pro g der Membranfraktion schwankten. Solche LH-ähnliche Substanzen wurden jedoch im menschlichen Skelettmuskelgewebe nicht gefunden. Es ist wahrscheinlich, daß eine dem LH-Typus ähnliche Aktivität ein indirekter Beweis für das Bestehen von LH-Rezeptoren in der menschlichen Prostata ist.

^* This work was supported by the Government Program PR-6

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