Thromb Haemost 1999; 81(04): 618-624
DOI: 10.1055/s-0037-1614535
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Schattauer GmbH

Phospholipase A2 Enzymes Regulate αIIbβ3-mediated, but not FcγRII Receptor-mediated, pp125FAK Phosphorylation in Platelets

Beatrice Haimovich
1   From the Department of Surgery, Robert Wood Johnson Medical School, Piscataway, NJ, Indianapolis, Indiana, USA
,
Ping Ji
,
Ernest Ginalis
,
Ruth Kramer
2   Lilly Research Laboratories, Indianapolis, Indiana, USA
,
Ralph Greco
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Publikationsverlauf

Publikationsdatum:
09. Dezember 2017 (online)

Summary

The αIIbβ3 integrin and FcγRII receptors mediate, respectively, platelet adhesion and spreading on fibrinogen and immunoglobulin (IgG) coated surfaces. Platelet adhesion to fibrinogen resulted in a partial conversion of the faster to the slower migrating (phosphorylated) form of Ca+2-sensitive cytosolic phospholipase A2 (cPLA2) but failed to trigger arachidonic acid (AA) release. Full mobility shift of cPLA2 and a massive release of AA release were stimulated by platelet adhesion to IgG or addition of thrombin to the fibrinogen adherent platelets. IgG and thrombin induced AA production were blocked by methyl arachidonyl fluorophosphonate (MAFP), an irreversible inhibitor of cPLA2 and the Ca+2-independent phospholipase A2 (iPLA2). In contrast, bromoenol lactone (BEL), a specific inhibitor of iPLA2 had no effect on the release of AA. MAFP and BEL prevented pp125FAK phosphorylation and platelet spreading on fibrinogen having no effect on pp125FAK phosphorylation or platelet spreading on immobilized IgG. We conclude that αIIbβ3-mediated pp125FAK phosphorylation and platelet spreading on fibrinogen are regulated by PLA2 enzymes.

Abbreviations: cPLA2, cytosolic calcium-dependent phospholipase A2; iPLA2, calcium-independent phospholipase A2; BSA, bovine serum albumin; mAB, monoclonal antibody; MAFP, methyl arachidonyl fluorophosphonate; pp125FAK, focal adhesion kinase; BEL, bromoenol lactone; AA, arachidonic acid.

 
  • References

  • 1 Lauffenburger DA, Horwitz AF. Cell migration: A physically integrated molecular process. Cell 1996; 84: 359-69.
  • 2 Dedhar S, Hannigan GE. Integrin cytoplasmic interactions and bidirectional transmembrane signaling. Current opinion in cell biology 1996; 8: 657-69.
  • 3 Burridge K, Chrzanowska-Wodnicka M. Focal adhesions, contractility, and signaling. Annu Rev Cell Biol 1996; 12: 463-519.
  • 4 Giancotti FG. Integrin signaling: specificity and control of cell survival and cell cycle progression. Current opinion in cell biology 1997; 9: 691-700.
  • 5 Coller B. Platelets in cardiovascular thrombosis and thrombolysis. In The heart and cardiovascular system edited by Fozzard HA. New York: Raven Press Ltd; 1992. p 219-73.
  • 6 Phillips DR, Charo IF, Scarborough RM. GP IIb-IIIa: The responsive integrin. Cell 1991; 65: 359-62.
  • 7 Nachmias SP, Golla R. Vinculin in relation to stress fibers in spread platelets. Cell Motil Cytoskeleton 1991; 20: 190-202.
  • 8 Haimovich B, Lipfert L, Brugge JS, Shattil SJ. Tyrosine phosphorylation and cytoskeletal reorganization in platelets are triggered by interaction of integrin receptors with their immobilized ligands. JBC 1993; 268: 15868-77.
  • 9 Haimovich B, Regan C, DiFazio L, Ginalis E, Ji P, Purohit U, Rowley RB, Bolen J, Greco R. The FcγRII receptor triggers pp125FAK phosphorylation in platelets. JBC 1996; 271: 16332-7.
  • 10 Leslie CC. Properties and regulation of cytosolic phospholipase A2 . JBC 1997; 272: 16709-12.
  • 11 Kramer RM, Roberts EF, Um SL, Borsch-Haubold AG, Watson SP, Fisher MJ, Jakubowski JA. p38 mitogen-activated protein kinase phosphorylates cytosolic phospholipase A2 (cPLA2) in thrombin-stimulated platelets. JBC 1996; 271: 27723-9.
  • 12 Chun JS, Jacobson BS. Spreading of HeLa cells on a collagen substratum requires a second messenger formed by the lipoxygenase metabolism of arachidonic acid released by collagen receptor clustering. Molec Biol Cell 1992; 3: 481-92.
  • 13 Clark EA, Hynes RO. Ras activation is necessary for integrin-mediated activation of extracellular signal-regulated kinase 2 and cytosolic phospholipase A2 but not for cytoskeletal organization. JBC 1996; 271: 14814-8.
  • 14 Mounier C, Faili A, Vargaftig B Bon Cassian, Hatmi M. Secretory phospholipase A2 is not required for arachidonic acid liberation during platelet activation. Eur J Biochem 1993; 216: 169-75.
  • 15 Huang Z, Payette P, Abdullah K, Cromlish WA, Kennedy BP. Functional identification of the active-site nucleophile of the human 85-kDa cytosolic phospholipase A2 . Biochemistry 1996; 35: 3712-21.
  • 16 Kramer RM, Roberts EF, Manetta JV, Hyslop PA, Jakubowski JA. Thrombin-induced phosphorylation and activation of Ca+2 -sensitive cytosolic phospholipase A2 in human platelets. JBC 1993; 268: 26796-804.
  • 17 Lin LL, Wartmann M, Lin AY, Knopf JL, Seth A, Davis RJ. cPLA2 is phosphorylated by MAP kinase. Cell 1993; 72: 269-78.
  • 18 Kramer RM, Roberts EF, Hyslop PA, Utterback BG, Hui KY, Jakubowski JA. Differential activation of cytosolic phospholipase A2 (cPLA2) by thrombin and thrombin receptor agonist peptide in human platelets. JBC 1995; 270: 14816-23.
  • 19 Borsch-Haubold AG, Kramer RM, Watson SP. Cytosolic phospholipase A2 is phosphorylated in collagen- and thrombin-stimulated human platelets independent of protein kinase C and mitogen-activated protein kinase. JBC 1995; 270: 25885-92.
  • 20 Kramer RM, Roberts EF, Strifler BA, Johnstone EM. Thrombin induces activation of p38 MAP kinase in human platelets. JBC 1995; 270: 27395-8.
  • 21 Lio YC, Reynolds LJ, Balsinde J, Dennis EA. Irreversible inhibition of Ca+2 -independent phospholipase A2 by methyl arachidonyl fluorophosphate. BBA 1996; 1302: 55-60.
  • 22 Balsinde J, Dennis EA. Distinct roles in signal transduction for each of the phospholipase A2 enzymes present in P388D1 macrophages. JBC 1997; 271: 6758-65.
  • 23 Teslenko V, Rogers M, Lefkowith JB. Macrophage arachidonate release via both the cytosolic Ca+2 -dependent and -independent phospholipases is necessary for cell spreading. BBA 1997; 1344: 189-99.
  • 24 Balboa MA, Blasinde J, Jones SS, Dennis EA. Identity between the Ca+2- independent phospholipase A2 enzymes from P388D1 macrophages and Chinese hamster ovary cells. JBC 1997; 272: 8576-80.
  • 25 Shattil SJ, Haimovich B, Cunningham M, Lipfert L, Parsons JT, Ginsberg MH, Brugge JS. Tyrosine phosphorylation of pp125FAK in platelets requires coordinated signaling through integrin and agonist receptors. JBC 1994; 269: 14738-45.
  • 26 Yanaga F, Asselin J, Schieven GL, Watson SP. Phenylarsine oxide inhibits tyrosine phosphorylation of phospholipase Cγ2 in human platelets and phospholipase Cγ2 in NIH-3T3 fibroblasts. Federation of European Biochemical Societies 1995; 368: 377-80.
  • 27 Liao F, Shin HS, Rhee SG. Cross-linking of FcγRIIIA on natural killer cells results in tyrosine phosphorylation of PLCγ1 and PLCγ2. Journal of Immunology 1993; 150: 2668-74.
  • 28 Siess W, Siegel FL, Lapetina EG. Arachidonic acid stimulates the formation of 1,2-diacylglycerol and phosphatidic acid in human platelets. JBC 1983; 258: 11236-42.
  • 29 Haimovich B, Kaneshiki N, Ji P. Protein kinase C regulates tyrosine phosphorylation of pp125FAK triggered in platelets adherent to fibrinogen. Blood 1996; 87: 152-61.
  • 30 Hwang SC, Jhon D-Y, Bae YS, Kim JH, Rhee SG. Activation of phospholipase C-γ by the concerted action of tau proteins and arachidonic acid. JBC 1996; 271: 18342-9.
  • 31 Liscovitch M, Cantley LC. Lipid second messengers. Cell 1994; 7: 329-34.
  • 32 Aris JP, Basta PV, Holmes WD, Ballas LM, Moomaw C, Rankl NB, Blobel G, Loomis CR, Burns DJ. Molecular and biochemical characterization of a recombinant human PKC-δ family member. BBA 1993; 1174: 171-81.
  • 33 Khan WA, Blobe G, Halpern A, Taylor W, Wetsel WC, Burns D, Lómmis C, Hannun YA. Selective regulation of protein kinase C isoenzymes by oleic acid in human platelets. JBC 1993; 268: 5063-8.
  • 34 Ackermann EJ, Kempner ES, Dennis EA. Ca+2 -independent cytosolic phospholipase A2 from macrophage like P388D1 cells. JBC 1994; 269: 9227-33.
  • 35 Hazen SL, Gross RW. Human myocardial cytosolic Ca+2 -independent phospholipase A2 is modulated by ATP. Concordant ATP-induced alterations in enzyme kinetics and mechanism-based inhibition. Biochem J 1991; 280: 581-7.
  • 36 Hirashima Y, Farooqui AA, Mills JS, Horrocks LA. Identification and purification of calcium-independent phospholipase A2 from bovine brain cytosol. Journal of Neurochemistry 1992; 59: 708-14.
  • 37 Portilla D, Dai G. Purification of a novel calcium-independent phospholipase A2 from rabbit kidney. JBC 1996; 271: 15451-7.
  • 38 Tang J, Kriz RW, Wolfman N, Shaffer M, Seehra M, Seehra J, Jones SS. A novel cytosolic calcium-independent phospholipase A2 contains eight ankyrin motifs. J Cell Biol 1997; 272: 8567-75.
  • 39 Seufferlein T, Rozengurt E. Lysophosphatidic acid stimulates tyrosine phosphorylation of focal adhesion kinase, Paxillin, and p130. JBC 1994; 269: 9345-51.
  • 40 Smyth SS, Hillery CA and Parise LV. Fibrinogen binding to purified platelet glycoprotein IIb-IIIa (integrin alpha IIb beta 3) is modulated by lipids. JBC 1992; 267: 15568-77.
  • 41 Ridley AJ, Hall A. The small GTP-binding protein rho regulates the assembly of focal adhesion and actin stress fibers in response to growth factors. Cell 1992; 70: 389-99.
  • 42 Peppelenbosch MP, Qiu R-G, de Vries-Smits AMM, Tertoolen LGJ, de Laat SW, McCormick F, Hall A, Symons MH, Bos JL. Rac mediates growth factor-induced arachidonic acid release. Cell 1998; 81: 849-56.
  • 43 Ridley AJ, Paterson HF, Johnston CL, Diekmann D, Hall A. The small GTP-binding protein rac regulates growth factor-induced membrane ruffling. Cell 1992; 70: 401-10.
  • 44 Nobes CD, Hall A. Rho, Rac, and Cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 1995; 81: 53-62.
  • 45 Hildebrand JD, Taylor JM, and Parsons JT. An SH3 domain-containing GT-Pase-activating protein for Rho and Cdc42 associates with focal adhesion.