Download PDF
Thromb Haemost 1999; 81(02): 313-314
DOI: 10.1055/s-0037-1614463
Letters to the Editor
Schattauer GmbH

Multiplex PCR for One-step Determination of the G20210A Variation and the Factor V Leiden Mutation by Denaturing Gradient Gel Electrophoresis (DGGE)

Sylvie Patri
1   From the Laboratoire de Génétique Cellulaire et Moléculaire, UPRES EA 2622, CHU de Poitiers, France
,
Sandra Salmeron
1   From the Laboratoire de Génétique Cellulaire et Moléculaire, UPRES EA 2622, CHU de Poitiers, France
,
Mickaël Caillon
1   From the Laboratoire de Génétique Cellulaire et Moléculaire, UPRES EA 2622, CHU de Poitiers, France
,
Alain Kitzis
1   From the Laboratoire de Génétique Cellulaire et Moléculaire, UPRES EA 2622, CHU de Poitiers, France
,
Jean-Claude Chomel
› Author Affiliations
Further Information

Publication History

Received13 July 1998

Accepted after revision06 November 1998

Publication Date:
08 December 2017 (online)

Also available at
    Permissions and Reprints

 

 

  • References

  • 1 Bertina RM, Koeleman BP, Koster T, Rosendaal FR, Dirven RJ, De Ronde H, Van der Velden PA, Reitsma PH. Mutation in blood coagulation factor V associated with resistance to activated protein C. Nature 1994; 369: 64-7.
    CrossrefPubMedSearch in Google Scholar
  • 2 Poort SR, Rosendaal FR, Reitsma PH, Bertina RM. A common genetic variation in the 3’-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis. Blood 1996; 88: 3698-703.
    PubMedSearch in Google Scholar
  • 3 Zöller B, Hillarp A, Berntorp E, Dahlbäck B. Activated protein C resistance due to a common factor V gene mutation is a major risk factor for venous thrombosis. Annual Rev Med 1997; 48: 45-58.
    CrossrefPubMedSearch in Google Scholar
  • 4 Rosendaal FR, Koster T, Vandenbroucke JP, Reitsma PH. High risk of thrombosis in patients homozygous for factor V Leiden (activated protein C resistance). Blood 1995; 85: 1504-8.
    PubMedSearch in Google Scholar
  • 5 Hézard N, Cornillet-Lefebvre P, Gillot L, Potron G, Nguyen P. Multiplex ASA PCR for a simultaneous determination of factor V Leiden gene, G→A 20210 prothrombin gene and C→T 677 MTHFR gene mutations. Thromb Haemost 1998; 79: 1054-5.
    Thieme ConnectPubMedSearch in Google Scholar
  • 6 Gomez E, Van Der Poel SCPAM, Jansen JH, Löwenberg B. Rapid simultaneous screening of factor V Leiden and G20210A prothrombin variant by multiplex polymerase chain reaction on whole blood. Blood 1998; 91: 2208-11.
    PubMedSearch in Google Scholar
  • 7 Ripoll L, Paulin D, Thomas S, Drouet LO. Multiplex PCR-mediated site-directed mutagenesis for one-step determination of factor V Leiden and G20210A transition of the prothrombin gene. Thromb Haemost 1997; 90: 960-1.
    Thieme ConnectPubMedSearch in Google Scholar
  • 8 Bowen DJ, Bowley S, John M, Collins PW. Factor V Leiden (G1691A), the prothrombin 3’-untranslated region variant (G20210A) and thermolabile methylenetetrahydrofolate reductase (C677T): A single genetic test genotypes all three loci-determination of frequancies in the S. Wales population of the UK. Thromb Haemost 1998; 79: 949-54.
    Thieme ConnectPubMedSearch in Google Scholar
  • 9 Patri S, Chomel JC, Salmeron S, Boinot C, Laneelle D, Sultan Y, Kitzis A. Detection of the 20210 A/G variation in the prothrombin gene by Denaturing Gradient Gel Electrophoresis (DGGE). Thromb Haemost 1998; 79: 1055-6.
    Thieme ConnectPubMedSearch in Google Scholar