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Thromb Haemost 1999; 82(03): 1153-1159
DOI: 10.1055/s-0037-1614345
Letters to the Editor
Schattauer GmbH

Collagen or Collagen-related Peptide Cause [Ca2+]i Elevation and Increased Tyrosine Phosphorylation in Human Megakaryocytes

J. C. Mountford   *
1   Department of Pharmacology, University of Oxford, Oxford
,
S. K. Melford
1   Department of Pharmacology, University of Oxford, Oxford
,
C. M. Bunce
2   Department of Medicine, University of Birmingham, Q. E. Medical Centre, Birmingham
,
J. Gibbins
3   School of Animal and Microbial Sciences, University of Reading, Whiteknights, Reading, UK
,
S. P. Watson
1   Department of Pharmacology, University of Oxford, Oxford
› Author Affiliations
Further Information

Publication History

Received 08 July 1998

Accepted 04 May 1999

Publication Date:
09 December 2017 (online)

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Summary

Since megakaryocytes are the cellular precursors of platelets we have investigated whether they share responses to platelet agonists, in particular collagen. Although previous studies have reported responses to thrombin in non-human megakaryocytes, through studies of single cell calcium responses and protein tyrosine-phosphorylation we demonstrate for the first time that both isolated human megakaryocytes and CD41/61-positive megakaryocytes derived in culture from CD34+ cells share responses to the platelet agonists collagen, collagen-related peptide and thrombin. The responses to either collagen or CRP were seen only in the most mature megakaryocytes and not in mega-karyocyte-like cell lines, suggesting that the response to collagen is a characteristic developed late during megakaryocyte differentiation. These primary cells offer the opportunity to use many molecular and cellular techniques to study and manipulate signalling events in response to platelet receptor agonists, which cannot be performed in the small, anucleate platelet itself.

* Current address: Dr. J. C. Mountford, Department of Medicine, University of Birmingham, Q. E. Medical Centre, Birmingham, B15 2TH, UK.


 

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