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Thromb Haemost 2000; 84(06): 1045-1051
DOI: 10.1055/s-0037-1614169
Review Article
Schattauer GmbH

Monitoring Hypocoagulant Conditions in Rat Plasma: Factors Determining the Endogenous Thrombin Potential of Tissue Factor-Activated Plasma

Cécile M. A. Nieuwenhuys
1   From the Department of Human Biology, The Netherlands
,
Marion A. H. Feijge
2   The Department of Biochemistry, University of Maastricht, The Netherlands
,
Suzette Béguin
2   The Department of Biochemistry, University of Maastricht, The Netherlands
,
Johan W. M. Heemskerk
1   From the Department of Human Biology, The Netherlands
2   The Department of Biochemistry, University of Maastricht, The Netherlands
› InstitutsangabenWe thank R. F. G. Offermans and R. van Oerle for analytical assistance. We thank Drs. H. C. Hemker, G. Hornstra and G. Tans for valuable comments and discussions. We acknowledge Dr. D. Rijkers for preparation of MVA-pNA. This study was supported by Hoffmann-La Roche (Basel, Switzerland).
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Publikationsverlauf

Received 24. Dezember 1999

Accepted after resubmission 29. Juni 2000

Publikationsdatum:
13. Dezember 2017 (online)

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Summary

Automated human plasma, continuous monitoring of the formation and inactivation of thrombin during the coagulation process provides an adequate way to detect hypo- and hypercoagulant conditions. Here, we describe an analogous procedure to determine the endogenous thrombin potential (ETP), i. e. the free thrombin concentration-time integral, of coagulating rat plasma. When activated with tissue factor, the ETP of plasma from Wistar rats was comparable to the ETP of human plasma, in spite of a relatively short half-life time of free thrombin in rat plasma. The ETP was highly sensitive to heparin as well as to administration of vitamin K antagonist or feeding of the animals with a vitamin K-deficient diet. In plasma that was activated under sub-optimal conditions (reduced levels of tissue factor or vitamin K-dependent coagulation factors), the ETP increased with the rate of thrombin formation in the first minutes of the coagulation process. Since both parameters are dependent of the prothrombin concentration, it appears that this level plays an important role in determining both the initial and total activity of the coagulation system. Thus, automated measurement of free thrombin during the coagulation process of rat plasma allows a detailed analysis of hypocoagulability in this animal model.

Key words

Endogenous thrombin potential - hypocoagulability - rat - tissue factor - vitamin K
 

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