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Thromb Haemost 2003; 90(01): 140-146
DOI: 10.1055/s-0037-1613610
Cellular Proteolysis and Oncology
Schattauer GmbH

Immunohistochemical localization of tissue factor pathway inhibitor-2 in human tumor tissue

Marek Z. Wojtukiewicz
1   Departments of Oncology and Pathomorphology, Medical Academy, Bialystok, Poland
,
Ewa Sierko
1   Departments of Oncology and Pathomorphology, Medical Academy, Bialystok, Poland
,
Lech Zimnoch
2   Departments of Pathomorphology, Medical Academy, Bialystok, Poland
,
Leszek Kozlowski
1   Departments of Oncology and Pathomorphology, Medical Academy, Bialystok, Poland
,
Walter Kisiel
3   Department of Pathology, University of New Mexico, School of Medicine, Albuquerque, New Mexico, USA
› Author AffiliationsFinancial support: This work was supported in part by research grant 6 P05A 096 21 from the Polish Committee of Scientific Research (KBN) to M.Z.W., and in part by research grant HL-64119 from the National Institutes of Health (to W.K.).
Further Information

Publication History

Received 19 September 2002

Accepted after revision 14 March 2003

Publication Date:
07 December 2017 (online)

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Summary

The progression of neoplasms is frequently associated with thromboembolic complications. Coagulation proteins, particularly tissue factor (TF), have been shown to play a role in tumor growth and metastatic dissemination. TFPI is the principal inhibitor of TF-dependent pathway of blood coagulation, but previous studies failed to detect antigenic TFPI in cancer tissue. Recently, a second inhibitor of tissue factor dependent pathway, TFPI-2 (also known as placental protein 5 [PP5] or matrix-associated serine protease inhibitor [MSPI]), has been described. Information on the presence of TFPI-2 within the malignant tumor tissue still remains obscure, and thus the aim of this study was to evaluate the expression of TFPI-2 in loco in several different neoplasms. TFPI-2 expression was demonstrated by immu-nohistochemical procedures in neoplastic cells of laryngeal, breast, gastric, colon, pancreatic, renal and endometrial cancer, as well as glial neoplasms. The intensity of staining was not uniform, with higher intensity in more differentiated tumors. G3 breast, gastric, endometrial and colon cancer cells revealed populations of cells that were either TFPI-2 positive or negative. Gastric and renal cancer tissue exhibited the presence of TFPI-2 in tumor infiltrating macrophages. TFPI-2 was also observed in normal tissue of the breast, stomach, colon and pancreas. These data demonstrate that the expression of TFPI-2 diminishes with an increasing degree of malignancy, which may suggest a role for TFPI-2 in the maintenance of tumor stability and inhibition of the growth of neoplasms.

Keywords

Tissue factor pathway inhibitor-2 - tumor tissue - immunohisto-chemistry
 

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