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Thromb Haemost 2003; 89(04): 660-665
DOI: 10.1055/s-0037-1613572
Blood Coagulation, Fibrinolysis and Cellular Haemostasis
Schattauer GmbH

Measurement of tissue factor messenger RNA levels in leukocytes from patients in hypercoagulable state caused by several underlying diseases

Tomohiro Sase
1   Second Department of Internal Medicine, Mie University School of Medicine, Tsu
,
Hideo Wada
2   Department of Laboratory Medicine, Mie University School of Medicine, Tsu
,
Junji Nishioka
3   Clinical Laboratory, Mie University Hospital, Tsu
,
Yasunori Abe
3   Clinical Laboratory, Mie University Hospital, Tsu
,
Esteban C. Gabazza
4   Third Department of Internal Medicine, Mie University School of Medicine, Tsu
,
Hiroshi Shiku
1   Second Department of Internal Medicine, Mie University School of Medicine, Tsu
,
Koji Suzuki
1   Second Department of Internal Medicine, Mie University School of Medicine, Tsu
,
Sin Nakamura
1   Second Department of Internal Medicine, Mie University School of Medicine, Tsu
,
Tsutomu Nobori
2   Department of Laboratory Medicine, Mie University School of Medicine, Tsu
› Author Affiliations
Further Information

Publication History

Received 12 September 2002

Accepted after revision 12 January 2003

Publication Date:
07 December 2017 (online)

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Summary

In a preliminary study, we have demonstrated that tissue factor (TF) is immunohistochemically stained in monocytes from patients with disseminated intravascular coagulation (DIC) but not from healthy volunteers, and that leukocytes from DIC patients induce enhanced activated factor X (FXa) generation in the presence of a mixture of FVIIa, FX and Ca2+. Then, TF mRNA levels in leukocytes were measured to evaluate the role of TF in the pathophysiology of various diseases. TF mRNA levels in leukocytes were low in healthy volunteers but they were significantly increased in various diseases, especially in patients with infectious diseases, solid cancer, anemd hematopoietic tumors. TF mRNA levels in leukocytes were significantly higher in patients with high levels of C reactive protein (CRP) than in those with low CRP. TF mRNA levels were significantly higher in patients with DIC than in those without DIC. TF mRNA levels were well correlated with TF antigens in plasma and leukocytes. These findings suggest that the expression of TF mRNA in leukocytes is increased in various diseases and that this may play an important role in hypercoagulability or DIC.

Keywords

Tissue factor - messenger RNA - DIC - real time PCR - CRP
 

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