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Thromb Haemost 2003; 89(06): 1043-1051
DOI: 10.1055/s-0037-1613406
Wound Healing and Inflammation/Infection
Schattauer GmbH

Intercellular adhesion molecule-1 (ICAM-1) expression is upregulated by thrombin in human monocytes and THP-1 cells in vitro and in pregnant subjects in vivo

Peter Clark
3   Department of Transfusion Medicine, Ninewells Hospital, Dundee, Scotland, UK
,
Fiona Jordan
1   University Department of Obstetrics and Gynaecology, Royal Infirmary, Glasgow, Scotland, UK
,
Charles Pearson
2   Department of Haematology, Royal Infirmary, Glasgow, Scotland, UK
,
Isobel D.Walker
2   Department of Haematology, Royal Infirmary, Glasgow, Scotland, UK
,
Naveed Sattar
1   University Department of Obstetrics and Gynaecology, Royal Infirmary, Glasgow, Scotland, UK
,
Joanne Ellison
1   University Department of Obstetrics and Gynaecology, Royal Infirmary, Glasgow, Scotland, UK
,
Ian A.Greer
1   University Department of Obstetrics and Gynaecology, Royal Infirmary, Glasgow, Scotland, UK
› Author AffiliationsFinancial support: The research was funded, in part, by a grant from The Laurence Scott Trust. The sponsors of the study had no role in the study design, data collection, analysis or writing of the report.
Further Information

Publication History

Received 09 September 2002

Accepted after revision 24 March 2003

Publication Date:
08 December 2017 (online)

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Summary

Monocytes play a pivotal role in both the inflammatory and coagulation responses, which may be mediated through a variety of adhesion molecules on the cell surface, including intercellular adhesion molecule-1 (ICAM-1). Monocytes also possess thrombin receptors. In the current study, we have demonstrated that thrombin can upregulate ICAM-1 mRNA and induce ICAM-1 expression on the monocyte in vitro and that, in vivo, higher monocyte ICAM-1 expression is observed in pregnancy (which is characterised by a physiological increase in thrombin generation). In pregnant subjects, a positive correlation between monocyte ICAM-1 expression and a number of markers of vascular/thrombotic disease (including blood group, acquired activated protein C resistance and non-fasting plasma triglyceride levels) was observed. We also observed a significant relationship between monocyte ICAM-1 expression and soluble plasma ICAM-1 levels, which would be consistent with a contribution of monocytic ICAM-1 to the levels of free ICAM-1 observed in plasma during pregnancy.

Consistent with a role in fibrinogen binding, our preliminary in vivo results suggest that monocyte ICAM-1 expression may be a useful marker of the thrombotic/inflammatory response, although further work is required to assess the relationship of monocyte ICAM-1 expression in thrombotic disorders.

Keywords

Monocyte - ICAM-1 - thrombin - pregnancy
 

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