Thromb Haemost 2002; 88(05): 768-772
DOI: 10.1055/s-0037-1613300
Review Article
Schattauer GmbH

The In Vitro Analysis of the Coagulation Mechanism of Activated Factor VII Using Thrombelastogram

Chiharu Kawaguchi
1   Division of Neonatal Intensive Care, Nara Medical University Hospital, Kashihara, Nara, Japan
,
Yukihiro Takahashi
1   Division of Neonatal Intensive Care, Nara Medical University Hospital, Kashihara, Nara, Japan
,
Yae Hanesaka
1   Division of Neonatal Intensive Care, Nara Medical University Hospital, Kashihara, Nara, Japan
,
Akira Yoshioka
1   Division of Neonatal Intensive Care, Nara Medical University Hospital, Kashihara, Nara, Japan
› Author Affiliations
Further Information

Publication History

Received 04 March 2002

Accepted after resubmission 02 July 2002

Publication Date:
08 December 2017 (online)

Zoom Image

Summary

We investigated the effects of addition of recombinant activated coagulation factor VII (rFVIIa) to coagulation factor-deficient plasma and whole blood, using thrombelastograms (TEGs). The addition of rFVIIa to factor II-or X-deficient plasma did not correct hemostatic parameters, whereas it produced partial responses in factor V-, VIII-or IX-deficient plasma and good responses in factor VII-, XI-or XII-deficient plasma. Furthermore, the addition of rFVIIa and platelets (30-100 X 103/µl) to platelet-poor plasma produced marked corrections, producing TEGs similar to those of platelet-rich plasma. These results indicate that factors II and X are essential for the hemostatic effects of rFVIIa, and that factors V and VIII promote these effects. We believe that TEGs are, at present, one of the most useful tools for evaluating in vitro hemostatic effects of rFVIIa.